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GB/T 17999.6-2008: SPF chicken -- Microbiological surveillance -- Part 6: Enzyme-linked immunosorbent assay for SPF chicken
Status: Valid GB/T 17999.6: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 17999.6-2008 | English | 169 |
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SPF chicken -- Microbiological surveillance -- Part 6: Enzyme-linked immunosorbent assay for SPF chicken
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GB/T 17999.6-2008
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| GB/T 17999.6-1999 | English | 199 |
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SPF chicken--Embryo susceptibility test
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GB/T 17999.6-1999
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PDF similar to GB/T 17999.6-2008
Basic data | Standard ID | GB/T 17999.6-2008 (GB/T17999.6-2008) | | Description (Translated English) | SPF chicken -- Microbiological surveillance -- Part 6: Enzyme-linked immunosorbent assay for SPF chicken | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 7,732 | | Date of Issue | 2008-12-31 | | Date of Implementation | 2009-05-01 | | Older Standard (superseded by this standard) | GB/T 17999.5-1999 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.27 (Total No.140) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the technical requirements of the enzyme-linked immunosorbent assay. Indirect ELISA This section applies to the SPF chicken serum antibody detection of these pathogenic microorganisms: Haemophilus paragallinarum (Haemophilus), Mycoplasma gallisepticum (Mycoplasma gallisepticum), Mycoplasma synoviae (Mycoplasma synoviae), bird flu virus (Avian Influeza Virus), NDV (Newcastle Disease Virus), infectious bronchitis virus (Infectious Bronchitis Virus), infectious law 's. | GB/T 17999.6-2008: SPF chicken -- Microbiological surveillance -- Part 6: Enzyme-linked immunosorbent assay for SPF chicken
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SPF chicken Microbiological surveillance Part 6. Enzyme-linked immunosorbent assay for SPF chicken
ICS 11.220
B41
National Standards of People's Republic of China
Replacing GB/T 17999.5-1999
SPF chickens microbiological monitoring
Part 6. SPF chickens ELISA
Posted 2008-12-31
2009-05-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
GB/T 17999 "SPF chickens microbiological monitoring" is divided into 10 parts.
--- The first part 1. SPF chicken microbiological monitoring General;
Part --- Article 2. SPF chicken hemagglutination inhibition test;
Part --- Section 3. SPF chicken serum neutralization test;
Part --- Chapter 4. SPF chicken serum plate agglutination test;
Part --- Article 5. SPF chicken agar diffusion test;
--- Part 6. SPF chickens ELISA;
Part --- Article 7. SPF chicken embryo susceptibility testing;
Part --- Article 8. SPF chicken Salmonella testing;
--- Part 9. SPF chickens tube agglutination test;
Part --- Article 10. SPF chickens indirect immunofluorescence assay.
This section GB/T 17999 Part 6.
The partial revision of the reference to GB/T 18936-2003 "highly pathogenic avian influenza diagnostic techniques", GB/T 19167-2003 "infectious bursal
Disease Diagnosis ", NY/T 538-2002" infectious coryza diagnostic techniques ", OIE" terrestrial animals (mammals, birds and bees) diagnosis
Manual of Tests and Vaccines "the relevant provisions (fifth edition) of.
This Part replaces GB/T 17999.5-1999 "SPF chickens ELISA."
This section compared with the GB/T 17999.5-1999 The main changes are as follows.
--- Increased indirect enzyme-linked immunosorbent assay;
--- Added Appendix A "reagent preparation."
This section of Appendix A normative appendix.
This section proposed by the People's Republic of China Ministry of Agriculture.
This part of the National Animal Epidemic Prevention Standardization Technical Committee (SAC/TC181) centralized.
This section is drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China Animal Health and Epidemiology Center, Jinan Spa law
Adams Poultry Limited.
The main drafters of this section. song even East, Jiang Qian, Han Lingxia, satellite Shao, Zhu fruit, single Zhong Fang, Liu Jiasen Secretary Chand, Guodong Chun, Yu Haibo,
Mengqing Wen.
This part of the standard replaces the previous editions are.
--- GB/T 17999.5-1999.
SPF chickens microbiological monitoring
Part 6. SPF chickens ELISA
1 Scope
This section GB/T 17999 specifies the technical requirements ELISA test.
Indirect ELISA This section applies to SPF chicken serum antibody detection of the following pathogens. Haemophilus paragallinarum
Avian influenza virus (AvianInfluezaVirus), Newcastle disease virus (NewcastleDiseaseVirus), infectious bronchitis virus
(InfectiousBronchitisVirus), infectious bursal disease virus (InfectiousBursalDiseaseVirus), reticuloendothelial hyperplasia
Virus (ReticuloendotheliosisVirus), chicken infectious anemia virus (ChickenInfectiousAnaemiaVirus), avian reovirus
Virus (viral arthritis) (AvianReovirus), avian (AvianEncephalomyelitisVirus), infectious laryngotracheitis gas
Tube stomatitis virus (InfectiousLaryngotracheitisVirus), avian adenovirus Ⅲ group (EDS) (AvianAdenovirusGroupⅢ), shower
Ba-cell leukemia virus (LymphoidLeukosisVirus).
This section double-antibody sandwich enzyme-linked immunosorbent assay suitable for SPF chickens lymphoblastic leukemia virus P27 antigen detection.
Principle 2
Indirect enzyme-linked immunosorbent assay using known microbial antigens to detect unknown antibody, double antibody sandwich enzyme-linked immunosorbent assay has been adopted
Known unknown antigen antibody detection.
3 indirect ELISA
3.1 Reagents
3.1.1 The envelope antigen negative and positive control sera, goat anti-chicken LgG HRP, saved and used according to instructions.
3.1.2 Appendix coating liquid diluent, substrate solution, stop solution preparation method A.
3.2 Equipment
Microtiter plates, pipettes, 37 ℃ incubator, microplate reader.
3.3 Procedure
3.3.1 antigen-coated. the bird flu virus, infectious bronchitis virus, infectious bursal disease virus, infectious laryngotracheitis virus, new
City disease virus, Haemophilus paragallinarum, avian adenovirus Ⅲ group (EDS), Mycoplasma gallisepticum, Mycoplasma synoviae, Avian encephalomyelitis virus, lymphocytic
Leukemia virus, reticuloendothelial hyperplasia virus, avian reovirus (viral arthritis), chicken infectious anemia virus antigen coating buffer dilute
Diluted to working concentration, was added to each well microtiter plates, each well 100μL, at 4 ℃ refrigerator overnight.
3.3.2 washing. rejection net hole antigen solution, with the washing liquid to fill the holes, place 5min, then dumped a net, so to repeat three times.
3.3.3 was tested serum plus. The dilution will be subject to 100 dilution of serum added to each well 100μL, each operation are set to negative control
Two holes, the positive control wells, blank comparison wells each, were added to the same negative serum dilution of positive serum and dilutions of each 100μL,
Plus different serum samples should change the tip, 37 ℃ incubator role 30min.
3.3.4 Washing. with 3.3.2.
3.3.5 plus goat anti-chicken IgG-HRP. The dilution of the enzyme-labeled antibody diluted to working concentration added to each well 100μL, 37 ℃ incubator
The role of 30min.
3.3.6 Washing. with 3.3.2.
3.3.7 color. the addition of solution 100μL, dark at room temperature for about 5min reaction (negative control wells start to produce a color when).
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