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GB/T 17999.4-2008: SPF chicken -- Microbiological surveillance -- Part 4: Serum plate agglutination test for SPF chicken
Status: Valid GB/T 17999.4: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 17999.4-2008 | English | 149 |
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SPF chicken -- Microbiological surveillance -- Part 4: Serum plate agglutination test for SPF chicken
| Valid |
GB/T 17999.4-2008
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| GB/T 17999.4-1999 | English | 199 |
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2 days [Need to translate]
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SPF chicken--Agar gel precipitation test
| Obsolete |
GB/T 17999.4-1999
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PDF similar to GB/T 17999.4-2008
Basic data | Standard ID | GB/T 17999.4-2008 (GB/T17999.4-2008) | | Description (Translated English) | SPF chicken -- Microbiological surveillance -- Part 4: Serum plate agglutination test for SPF chicken | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 5,599 | | Date of Issue | 2008-12-31 | | Date of Implementation | 2009-05-01 | | Older Standard (superseded by this standard) | GB/T 17999.3-1999 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.27 (Total No.140) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the chicken serum plate agglutination test technical requirements. This section applies to the SPF chicken serum antibody detection of pathogenic microorganisms in the following: Salmonella pullorum (Salmonella pullorum), Mycoplasma gallisepticum (Mycoplasma gallisepticum), Mycoplasma synoviae (Mycoplasma synoviae), Haemophilus paragallinarum (Haemophilus paragauinarum). | GB/T 17999.4-2008: SPF chicken -- Microbiological surveillance -- Part 4: Serum plate agglutination test for SPF chicken
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
SPF chicken Microbiological surveillance Part 4. Serum plate agglutination test for SPF chicken
ICS 11.220
B41
National Standards of People's Republic of China
Replacing GB/T 17999.3-1999
SPF chickens microbiological monitoring
Part 4. SPF chicken serum plate agglutination test
Posted 2008-12-31
2009-05-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
GB/T 17999 "SPF chickens microbiological monitoring" is divided into 10 parts.
--- The first part 1. SPF chicken microbiological monitoring General;
Part --- Article 2. SPF chicken hemagglutination inhibition test;
Part --- Section 3. SPF chicken serum neutralization test;
Part --- Chapter 4. SPF chicken serum plate agglutination test;
Part --- Article 5. SPF chicken agar diffusion test;
--- Part 6. SPF chickens ELISA;
Part --- Article 7. SPF chicken embryo susceptibility testing;
Part --- Article 8. SPF chicken Salmonella testing;
--- Part 9. SPF chickens tube agglutination test;
Part --- Article 10. SPF chickens indirect immunofluorescence assay.
This section GB/T Part of 417,999.
The partial revision of the reference to the NY/T 553-2002 "avian mycoplasma disease diagnostic technology" and NY/T 536-2002 "fowl typhoid and pullorum
Relevant provisions of the diagnostic techniques "in.
This Part replaces GB/T 17999.3-1999 "SPF chicken serum plate agglutination test."
This section compared with the GB/T 17999.3-1999 The main changes are as follows.
--- Increased saline as a negative control.
This section proposed by the People's Republic of China Ministry of Agriculture.
This part of the National Animal Epidemic Prevention Standardization Technical Committee (SAC/TC181) centralized.
This section is drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China Animal Health and Epidemiology Center, Jinan Spa law
Adams Poultry Limited.
The main drafters of this section. song even East, Jiang Qian, Han Lingxia, satellite Shao, Zhu fruit, single Zhong Fang, Liu Jiasen Secretary Chand, Guodong Chun, Yu Haibo,
Mengqing Wen.
This part of the standard replaces the previous editions are.
--- GB/T 17999.3-1999.
SPF chickens microbiological monitoring
Part 4. SPF chicken serum plate agglutination test
1 Scope
This section GB/T 17999 specifies the technical requirements for chicken serum plate agglutination test.
Principle 2
Serum plate agglutination test is a bacterial antigen and the corresponding antibody binding, in the right amount of electrolyte participation, after a period of time appear
Visible agglutination, often using a known standard liquid bacterial antigens corresponding detection antibody agglutination.
3 Reagents and materials
3.1 Reagents
3.1.1 agglutination antigen.
3.1.2 negative sera, sera.
3.1.3 was tested serum.
3.2 Material
Glass pipette (TD10μL ~ 200μL) and tips.
Procedure 4
10min ~ 20min ago 4.1 Remove from the refrigerator antigen negative standard serum, the positive standard serum and serum were seized, to reach
Room temperature.
4.2 Mix well to draw a drop of diagnostic antigen (0.025mL ~ 0.05mL) with a pipette, vertical drops on the glass, then quickly
Next to dropping the same amount of antigen in the serum of the subject drop (0.025mL ~ 0.05mL).
4.3 with a toothpick serum mixed with the antigen coated sheet into 1cm ~ 2cm in diameter, constantly shaking the glass, 2min Vipassana
Police found.
4.4 The test shall be carried out at 20 ℃ ~ 25 ℃ conditions.
4.5 each batch of test serum should be set positive and negative serum and saline control.
5 results found
50% occurred within 2min (++) above as positive agglutination. No agglutination within 2min (-) were negative, between two of the above
Room as suspicious, reviewed the test, such as still suspicious, judged as positive. Agglutination interpretation criteria in Table 1.
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