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GB/T 17999.3-2008 English PDF

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GB/T 17999.3-2008: SPF chicken -- Microbiological surveillance -- Part 3: Serum neutralization test for SPF chicken
Status: Valid

GB/T 17999.3: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GB/T 17999.3-2008English229 Add to Cart 3 days [Need to translate] SPF chicken -- Microbiological surveillance -- Part 3: Serum neutralization test for SPF chicken Valid GB/T 17999.3-2008
GB/T 17999.3-1999English199 Add to Cart 2 days [Need to translate] SPF chicken--Serum plate agglutination test Obsolete GB/T 17999.3-1999

PDF similar to GB/T 17999.3-2008


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Basic data

Standard ID GB/T 17999.3-2008 (GB/T17999.3-2008)
Description (Translated English) SPF chicken -- Microbiological surveillance -- Part 3: Serum neutralization test for SPF chicken
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 10,135
Date of Issue 2008-12-31
Date of Implementation 2009-05-01
Older Standard (superseded by this standard) GB/T 17999.2-1999
Regulation (derived from) National Standard Approval Announcement 2008 No.27 (Total No.140)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the technical requirements of the serum neutralization test. This section applies to the following SPF chickens and virus detection antibody: avian encephalomyelitis virus (Avian Encephalomyelitis Virus), infectious bronchitis virus (Infectious Bronchitis Virus), ILTV (Infectious Laryngotracheitis Virus), infectious bursal disease virus (Infectious Bursal Disease Virus).

GB/T 17999.3-2008: SPF chicken -- Microbiological surveillance -- Part 3: Serum neutralization test for SPF chicken


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
SPF chicken Microbiological surveillance Part 3. Serum neutralization test for SPF chicken ICS 11.220 B41 National Standards of People's Republic of China Replacing GB/T 17999.2-1999 SPF chickens microbiological monitoring Part 3. SPF chicken serum neutralization test Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 17999 "SPF chickens microbiological monitoring" is divided into 10 parts. --- The first part 1. SPF chicken microbiological monitoring General; Part --- Article 2. SPF chicken hemagglutination inhibition test; Part --- Section 3. SPF chicken serum neutralization test; Part --- Chapter 4. SPF chicken serum plate agglutination test; Part --- Article 5. SPF chicken agar diffusion test; --- Part 6. SPF chickens ELISA; Part --- Article 7. SPF chicken embryo susceptibility testing; Part --- Article 8. SPF chicken Salmonella testing; --- Part 9. SPF chickens tube agglutination test; Part --- Article 10. SPF chickens indirect immunofluorescence assay. This section GB/T 17999 Part 3. The partial revision of the reference to the OIE "terrestrial animals (mammals, birds and bees) diagnostic tests and vaccines Handbook" (fifth edition) the relevant Provisions. This Part replaces GB/T 17999.2-1999 "SPF chicken serum neutralization test." This section compared with the GB/T 17999.2-1999 The main changes are as follows. --- Increasing the normative Appendix A "reagent preparation," informative Appendix B "virus inoculation," as well as information appendix C "chick pick Species characteristic lesions after virus "; --- Part in the test content, increasing dilutions of the virus; --- Redefine different viruses lethal chick time. Appendix A to this part of the normative appendix, Appendix B, Appendix C is informative appendix. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Animal Epidemic Prevention Standardization Technical Committee (SAC/TC181) centralized. This section is drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China Animal Health and Epidemiology Center, Jinan Spa law Adams Poultry Limited. The main drafters of this section. song even East Liujia Sen, Han Lingxia, satellite Shao, Zhu fruit, single Zhong Fang, Jiang Qian, Secretary Chand, Yu Haibo, Mengqing Wen. This part of the standard replaces the previous editions are. --- GB/T 17999.2-1999. SPF chickens microbiological monitoring Part 3. SPF chicken serum neutralization test

1 Scope

This section GB/T 17999 specifies the technical requirements and test serum. This section applies to the following SPF chickens virus neutralizing antibody detection. AEV (AvianEncephalomyelitis Virus), infectious bronchitis virus (InfectiousBronchitisVirus), infectious laryngotracheitis virus (InfectiousLaryngo- tracheitisVirus), infectious bursal disease virus (InfectiousBursalDiseaseVirus). Principle 2 Virus neutralization test means an antibody binding with the corresponding biological activity, the loss of their biological activity and response. And Anti Should not only highly specific, and has a strict relation between the amount of. And therefore can be used in tests to identify the virus, it can also be used to quantify the relative antibodies Testing.

3 Reagents and Equipment

3.1 Reagents 3.1.1 Virus. VanRoekel avian strain (CVCCAV35), infectious bronchitis virus H52 (CVCC AV1513), infectious bursal disease virus strain A80 (CVCVAV2321), infectious laryngotracheitis virus A96 (CVCCAV200). 3.1.2 virus dilutions. tryptone phosphate broth (see Appendix A). 3.1.3 penicillin/streptomycin (double-antibody) solution (see Appendix A). 3.1.4 3% iodine solution. 3.1.5 SPF embryo. 3.2 Equipment 3.2.1 Egg drilling (hole punch). 3.2.2 6 needles. 3.2.3 7 needles. 3.2.4 mixer. 3.2.5 Eggtester. Procedure 4 4.1 Test preparation 4.1.1 serum sample Subject serum and positive control sera 56 ℃, 30min ~ 45min inactivated complement, stored at -20 ℃ condition standby. 4.1.2 SPF Peidan ready According embryo embryo viability check, select vessels clear, the embryo is healthy embryo swing law, each test item is required Peidan 24. 4.1.3 diluted virus 4.1.3.1 take sterile tubes 4 ~ 9, was added to each tube dilution 4mL, penicillin/streptomycin solution 0.5mL. 4.1.3.2 The first one added to the virus solution 0.5mL, for successive 10-fold dilution. Dilution of various viruses in Table 1.

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