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GB/T 17999.2-2008 English PDF

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GB/T 17999.2-2008: SPF chicken -- Microbiological surveillance -- Part 2: Hemagglutination inhibition test for SPF chicken
Status: Valid

GB/T 17999.2: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GB/T 17999.2-2008English209 Add to Cart 3 days [Need to translate] SPF chicken -- Microbiological surveillance -- Part 2: Hemagglutination inhibition test for SPF chicken Valid GB/T 17999.2-2008
GB/T 17999.2-1999English239 Add to Cart 2 days [Need to translate] SPF chicken--Serum neutralization test Obsolete GB/T 17999.2-1999

PDF similar to GB/T 17999.2-2008


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Basic data

Standard ID GB/T 17999.2-2008 (GB/T17999.2-2008)
Description (Translated English) SPF chicken -- Microbiological surveillance -- Part 2: Hemagglutination inhibition test for SPF chicken
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 9,954
Date of Issue 2008-12-31
Date of Implementation 2009-05-01
Older Standard (superseded by this standard) GB/T 17999.1-1999
Regulation (derived from) National Standard Approval Announcement 2008 No.27 (Total No.140)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the technical requirements of hemagglutination inhibition tests. This section applies to the following SPF chickens pathogenic microorganisms hemagglutination inhibition antibody test: the avian flu virus (Avian InfluenzaVirus), infectious bronchitis virus (Infectious Bronchitis Virus), NDV (Newcastle Disease Virus), avian adenovirus �� group (egg drop syndrome virus) (Avian Adenovirus Group ��), mycoplasma gallisepticum (Mycoplasma gallisepticum).

GB/T 17999.2-2008: SPF chicken -- Microbiological surveillance -- Part 2: Hemagglutination inhibition test for SPF chicken


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
. SPF chicken Microbiological surveillance Part 2. Hemagglutination inhibition test for SPF chicken ICS 11.220 B41 National Standards of People's Republic of China Replacing GB/T 17999.1-1999 SPF chickens microbiological monitoring Part 2. SPF chicken hemagglutination inhibition test Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 17999 "SPF chickens microbiological monitoring" is divided into 10 parts. --- The first part 1. SPF chicken microbiological monitoring General; Part --- Article 2. SPF chicken hemagglutination inhibition test; Part --- Section 3. SPF chicken serum neutralization test; Part --- Chapter 4. SPF chicken serum plate agglutination test; Part --- Article 5. SPF chicken agar diffusion test; --- Part 6. SPF chickens ELISA; Part --- Article 7. SPF chicken embryo susceptibility testing; Part --- Article 8. SPF chicken Salmonella testing; --- Part 9. SPF chickens tube agglutination test; Part --- Article 10. SPF chickens indirect immunofluorescence assay. This section GB/T 17999 Part 2. The partial revision of the reference to GB/T 18936-2003 "highly pathogenic avian influenza diagnostic techniques", SN/T 1109-2002 "micro Newcastle The amount of hemagglutination inhibition test procedures ", SN/T 1182.2-2004" bird flu micro hemagglutination inhibition test " NY/T 551-2002 "egg drop syndrome diagnostic techniques", OIE "terrestrial animals (mammals, birds and bees) diagnostic tests and vaccines Handbook "relevant regulations (fifth edition) of. This Part replaces GB/T 17999.1-1999 "SPF chicken hemagglutination inhibition test." This section compared with the GB/T 17999.1-1999 The main changes are as follows. --- Added "1% chicken red blood cell suspension prepared" Normative Appendix A "Preparation of Reagents" and informative Appendix B; --- Increase the results of the V-shaped aperture plate hemagglutination test of determination; --- Avian influenza virus, Newcastle disease virus, infectious bronchitis virus, avian adenovirus Ⅲ group (EDS) Mycoplasma gallisepticum and synoviae Mycoplasma hemagglutination inhibition antibody criteria were modified. Appendix A of this section is normative appendix, Appendix B is an informative annex. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Animal Epidemic Prevention Standardization Technical Committee (SAC/TC181) centralized. This section is drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China Animal Health and Epidemiology Center, Jinan Spa law Adams Poultry Limited. The main drafters of this section. song even East Liujia Sen, Han Lingxia, satellite Shao, Zhu fruit, single Zhong Fang, Jiang Qian, Secretary Chand, Yu Haibo, Mengqing Wen. This part of the standard replaces the previous editions are. --- GB/T 17999.1-1999. SPF chickens microbiological monitoring Part 2. SPF chicken hemagglutination inhibition test

1 Scope

This section GB/T 17999 specifies the hemagglutination inhibition test technical requirements. This section applies to the following SPF chickens pathogenic microorganisms hemagglutination inhibition antibody test. avian influenza virus (AvianInfluenza Virus), infectious bronchitis virus (InfectiousBronchitisVirus), Newcastle disease virus (NewcastleDiseaseVirus), poultry Principle 2 Many animal pathogens agglutinate erythrocytes capability that RBC agglutination can be inhibited by specific antibodies, thus A known pathogen detection corresponding hemagglutination inhibition antibody.

3 Reagents and Equipment

3.1 Reagents 3.1.1 hemagglutinin antigen. 3.1.2 negative and positive sera. 3.1.3 was tested serum. 3.1.4 chicken erythrocytes. 3.1.5 0.85% sodium chloride (NaCl) (see Appendix A). 3.1.6 A's solution (see Appendix A). 3.2 Equipment 3.2.1 96-well microtiter plate hemagglutination (U or V shaped hole shaped hole). 3.2.2 Micropipettes (range is 25μL, 50μL, 100μL) or adjustable micropipette (range covering 25μL, 50μL, 100μL) And tips. 3.2.3 water bath. 3.2.4 graduated centrifuge tube and centrifuge. Procedure 4 4.1 Preparation of erythrocyte suspension and antigen HA titer determination 4.1.1 1% chicken red blood cell suspension prepared See Appendix B. 4.1.2 hemagglutinin antigen titer determination 4.1.2.1 96-well microtiter plate hemagglutination first hole - the 12th hole of normal saline 25μL, for a total of 2 rows. Each row of the first hole to join Antigen 25μL, with a micropipette, from the first hole - the 11th hole against the dilution of the original series. the upcoming first hole of antigen and saline With a pipette repeatedly pipetting 3 times, aspiration 25μL moved to the second hole, and then repeated three times after pipetting, aspiration 25μL moved to the third hole, according to these Push until the 11th hole. Withdrawing from the first 11 holes of 25μL discarded, and the hole antigen final dilution 1.2 11 (i.e. 11log2). Article 12 As a control bore hole.

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