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GB/T 17817-2024: Determination of vitamin A in feeds - High performance liquid chromatography
Status: Valid GB/T 17817: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 17817-2024 | English | 439 |
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Determination of vitamin A in feeds - High performance liquid chromatography
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GB/T 17817-2024
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| GB/T 17817-2010 | English | 209 |
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Determination of vitamin A in feeds -- High-performance liquid chromatography
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GB/T 17817-2010
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| GB/T 17817-1999 | English | 279 |
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Determination of vitamin A in feeds--High-pressure liquid chromatography
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GB/T 17817-1999
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PDF similar to GB/T 17817-2024
Basic data | Standard ID | GB/T 17817-2024 (GB/T17817-2024) | | Description (Translated English) | Determination of vitamin A in feeds - High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 22,215 | | Date of Issue | 2024-11-28 | | Date of Implementation | 2025-06-01 | | Older Standard (superseded by this standard) | GB/T 17817-2010 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 17817-2024: Determination of vitamin A in feeds - High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of vitamin A in feeds - High performance liquid chromatography
Determination of Vitamin A in Feed by High Performance Liquid Chromatography
Chromatography
ICS 65.120
CCS B 46
National Standard of the People's Republic of China
Replace GB/T 17817-2010
Released on 2024-11-28
2025-06-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface ... Ⅲ
1 Scope ... 1
2 Normative references ... 1
3 Terms and Definitions ... 1
4 The first method of saponification extraction ... 1
4.1 Principle 1
4.2 Reagents or Materials 1
4.3 Instruments and Equipment 2
4.4 Samples 2
4.5 Test steps 3
4.6 Experimental data processing 6
4.7 Precision 7
5 Second method direct extraction method ... 7
5.1 Principle 7
5.2 Reagents or Materials 7
5.3 Instruments and Equipment 7
5.4 Samples 8
5.5 Test steps 8
5.6 Experimental data processing 8
5.7 Precision 9
Appendix A (Normative) Mass concentration calibration method for standard stock solutions of vitamin A and vitamin A acetate ... 10
Appendix B (Informative) Switching Flowchart of Online Solid Phase Extraction Valve ... 11
Appendix C (Informative) Liquid Chromatogram of Vitamin A Standard Solution ... 12
Appendix D (Informative) Chromatogram of Vitamin A Acetate Standard Solution ... 14
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
This document replaces GB/T 17817-2010 "Determination of Vitamin A in Feed - High Performance Liquid Chromatography" and is compatible with GB/T 17817-
Compared with.2010, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
The scope of application has been changed, concentrate supplements and compound premixed feeds have been added, and the quantitative
Limited (see Chapter 1, Chapter 1 of the.2010 edition);
a)
Added the standard series of vitamin A solutions in the “First Method of Saponification Extraction” (see 4.2.19); b)
Change the sample preparation method of compound feed, concentrate supplement and concentrated feed in the "First Method Saponification Extraction Method" to
Pass through a 1 mm sieve (see 4.4, 3.5 of the.2010 edition);
c)
Change the extractant of "First Method Saponification Extraction Method" to "petroleum ether" (boiling range 30 ℃ ~ 60 ℃) (see 4.5.1.2.1,
3.6.1.2 of the.2010 edition);
d)
Added off-line solid phase extraction method (see 4.5.1.2.2) and on-line solid phase extraction method (see 4.5.1.2.3); e)
Deleted normal phase chromatography conditions (see 3.6.2.1.1 of the.2010 edition); f)
Added liquid chromatography reference conditions (see 4.5.2.2 and 4.5.2.3); g)
Added qualitative detection method, and added multi-point calibration for quantitative detection (see 4.5.2.5, 4.5.2.6); h)
The experimental data processing in the “First Method Saponification Extraction Method” has been changed (see 4.6, 3.6.2.3.1 of the.2010 edition); i)
Added vitamin A standard series solution in the “Second Method Direct Extraction Method” (see 5.2.5); j)
The extraction temperature of the “second method direct extraction method” was changed from 65°C to room temperature (see 5.5.1, 4.6.1 of the.2010 edition); k)
Changed the experimental data processing in "Method 2 Direct Extraction Method" (see 5.6, 4.6.2.3 of the.2010 edition); l)
Added the method for calibrating the mass concentration of vitamin A and vitamin A acetate standard stock solution (see Appendix A); m)
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC 76).
This document was drafted by. Institute of Agricultural Quality Standards and Testing Technology, Chinese Academy of Agricultural Sciences, Shandong Provincial Livestock Product Quality and Safety Center,
DSM Vitamins (Shanghai) Co., Ltd., Sichuan Well Testing Technology Co., Ltd., China Animal Husbandry Industry Co., Ltd., Guangzhou Aibao
Agribiotech Ltd.
The main drafters of this document are. Zhao Xiaoyang, Song Rong, Yu Zhegao, Zhang Fengping, Liu Xiangyang, Zhu Gaoqun, Liu Zhiying, Guo Hongshuang, Wang Zhongyan,
Zhang Wei, Xie Li, Li Libei, Zhang Hui, Song Yan, Feng Xiuyan, and Cao Lin.
The previous versions of this document and the documents it replaces are as follows.
First published in.1999 as GB/T 17817-1999, first revised in.2010; -
This is the second revision. -
Determination of Vitamin A in Feed by High Performance Liquid Chromatography
Chromatography
1 Scope
This document describes a method for the determination of vitamin A in feeds by HPLC.
The "first method of saponification extraction" in this document is applicable to compound feed, concentrate supplement, concentrated feed, compound premix feed, vitamin
Determination of vitamin A in vitamin premix feed; "Method 2 direct extraction method" is applicable to compound premix feed, vitamin premix feed
Determination of vitamin A acetate in feed.
The first limit of quantification in this document is 1 000 IU/kg, and the second limit of quantification is 20 000 IU/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies.
in this document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4.Method 1.Saponification Extraction
4.1 Principle
The sample is saponified with an alkaline solution, purified by liquid-liquid extraction or solid-phase extraction, separated and determined by high performance liquid chromatography, and quantified by the external standard method.
4.2 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
4.2.1 Water. GB/T 6682, Grade 1.
4.2.2 Anhydrous ethanol. chromatographic grade.
4.2.3 Petroleum ether (boiling range 30 ℃ ~ 60 ℃).
4.2.4 Methanol. chromatographic grade.
4.2.5 Acetonitrile. chromatographic grade.
4.2.6 Isopropanol. chromatographic grade.
4.2.7 95% ethanol.
4.2.8 L﹘ascorbic acid.
4.2.9 Butylated hydroxytoluene (BHT).
4.2.10 Anhydrous sodium sulfate.
4.2.11 Potassium hydroxide solution (500 g/L). Weigh 500 g potassium hydroxide, dissolve it in water, cool it down, make up to 1 L with water, and mix well.
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