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GB/T 17778-2025: Determination of D-biotin in feed additive premix
Status: Valid GB/T 17778: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 17778-2025 | English | 189 |
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Determination of D-biotin in feed additive premix
| Valid |
GB/T 17778-2025
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| GB/T 17778-2005 | English | 155 |
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Determination of d-biotin in premix
| Valid |
GB/T 17778-2005
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| GB/T 17778-1999 | English | 199 |
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Determination of d-biotin in compound premix
| Obsolete |
GB/T 17778-1999
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PDF similar to GB/T 17778-2025
Basic data | Standard ID | GB/T 17778-2025 (GB/T17778-2025) | | Description (Translated English) | Determination of D-biotin in feed additive premix | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 9,948 | | Date of Issue | 2025-06-30 | | Date of Implementation | 2026-01-01 | | Older Standard (superseded by this standard) | GB/T 17778-2005 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 17778-2025: Determination of D-biotin in feed additive premix---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT17778-2025
ICS 65.120
CCSB46
National Standard of the People's Republic of China
Replaces GB/T 17778-2005
Determination of D-biotin in premixed feed
Released on June 30, 2025
Implementation on January 1, 2026
State Administration for Market Regulation
The National Standardization Administration issued
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document replaces GB/T 17778-2005 "Determination of d-biotin in premixed feeds". Compared with GB/T 17778-2005,
In addition to structural adjustments and editorial changes, the main technical changes are as follows.
a) The scope of application has been changed, and the detection limit and quantification limit have been added (see Chapter 1, Chapter 1 of the.2005 edition);
b) The shelf life of standard solutions and standard curves have been added to the HPLC method (see 4.2.6, 4.2.7, and 4.2.8);
c) The principle, standard stock solution concentration, constant volume and calculation formula of HPLC method have been changed (see 4.1, 4.2.6, 4.5.1,
4.6, 4.1, 4.2.3.1, 4.5.1, and 4.6 of the.2005 edition);
d) The shelf life of standard solutions has been added to the spectrophotometric method (see 5.2.5 and 5.2.6);
e) The solvent, extraction conditions, reaction system and measurement wavelength have been changed in the spectrophotometric method (see 5.2, 5.5.1, 5.5.2,.2005 edition).
3.2, 3.5.1, 3.5.2).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76).
This document was drafted by. Guangzhou Huibiao Testing Technology Center and Zhejiang NHU Co., Ltd.
The main drafters of this document are. Hao Yanjuan, Luo Qun, Yang Jinshu, Wang Zhimin, Shi Dongming, Long Zhixi, Yang Yucheng, Luo Zifeng, Shen Darong,
Chen Xiaoqiang, Pan Huanyu, Zhang Yiming, Quan Huali, and Sui Yanmei.
The previous versions of this document and the documents it replaces are as follows.
--- First published as GB/T 17778-1999 in.1999 and first revised in.2005;
---This is the second revision.
Determination of D-biotin in premixed feed
1 Scope
This document describes the HPLC and spectrophotometric determination of D-biotin in vitamin premixes and compound premixes.
method.
This document is applicable to the determination of D-biotin in vitamin premix feeds and compound premix feeds.
The detection limit of the HPLC method in this document is 1 mg/kg, and the quantification limit is 3 mg/kg; the detection limit of the spectrophotometric method is
1.5 mg/kg, limit of quantification is 5 mg/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and Definitions
There are no terms or definitions that require definition in this document.
4 High-performance liquid chromatography
4.1 Principle
The D-biotin in the sample was extracted with water and determined by high performance liquid chromatography using the external standard method.
4.2 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
4.2.1 Water. GB/T 6682, Grade 1.
4.2.2 Acetonitrile. chromatographic grade.
4.2.3 Diethylenetriaminepentaacetic acid (DTPA).
4.2.4 5 mol/L sodium hydroxide solution. Weigh 20 g of sodium hydroxide, add appropriate amount of water to dissolve, cool to room temperature, and dilute to 100 mL.
4.2.5 0.05% trifluoroacetic acid solution. Measure 0.5 mL of trifluoroacetic acid, dilute to 1000 mL with water, and adjust with sodium hydroxide solution (4.2.4).
Adjust pH to 2.5.
4.2.6 Standard stock solution (0.2 mg/mL). Weigh 10 mg of D-biotin (CAS No. 58-85-5, purity not less than 98%) standard
(accurate to 0.01 mg) in a 50 mL volumetric flask, add water, ultrasonically dissolve, dilute to volume with water, and mix well. Store at 2°C~8°C.
3 months.
4.2.7 Standard intermediate solution (20 μg/mL). Accurately pipette 5 mL of D-biotin standard stock solution (4.2.6) into a 50 mL volumetric flask.
Dilute to volume with water. Store at 2°C~8°C. Valid for 2 months.
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