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GB/T 15399-2018: Determination of sulfur amino acids in feeds -- Ion exchange chromatography
Status: Valid GB/T 15399: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 15399-2018 | English | 199 |
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Determination of sulfur amino acids in feeds -- Ion exchange chromatography
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GB/T 15399-2018
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| GB/T 15399-1994 | English | 199 |
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Determination of sulfur amino acids in feedstuffs. Ion exchange chromatography
| Obsolete |
GB/T 15399-1994
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PDF similar to GB/T 15399-2018
Basic data | Standard ID | GB/T 15399-2018 (GB/T15399-2018) | | Description (Translated English) | Determination of sulfur amino acids in feeds -- Ion exchange chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 10,160 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Older Standard (superseded by this standard) | GB/T 15399-1994 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 15399-2018: Determination of sulfur amino acids in feeds -- Ion exchange chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of sulfur amino acids in feeds - Ion exchange chromatography
ICS 65.120
B46
National Standards of People's Republic of China
Replace GB/T 15399-1994
Determination of sulfur-containing amino acids in feed
Ion exchange chromatography
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces GB/T 15399-1994 "Ion exchange chromatography method for determination of sulfur-containing amino acids in feed".
Compared with GB/T 15399-1994, this standard is subject to the following technical changes as follows.
--- The applicable objects in the range increase the concentrate replenishment and increase the limit of quantitation of the method (see Chapter 1);
--- Revised the expression of the principle (see Chapter 3, Chapter 3 of the.1994 edition);
--- Added phenol to the peroxyacid oxidizing solution (see 4.5,.1994 edition 4.1);
--- Give warning when partial sodium sulfite is used as a terminator (see 7.1.2);
--- The determination of the resolution of the methionine sulfone and the size of the peak area of the sample (see 7.2);
--- Modified the calculation formula in the test data processing (see Chapter 8);
--- Added chromatogram of the amino acid mixing standard solution (see Appendix A).
This standard is proposed and managed by the National Feed Industry Standardization Technical Committee (SAC/TC76).
This standard was drafted. Institute of Agricultural Quality Standards and Testing Technology, Chinese Academy of Agricultural Sciences [National Feed Quality Supervision and Inspection Center
(Beijing)], Tongwei Co., Ltd.
The main drafters of this standard. Zhao Genlong, Jia Wei, Li Lan, Song Jun, Zhao Yan, Zhang Fengqi.
The standard version of the standard is replaced by this standard.
---GB/T 15399-1994.
Determination of sulfur-containing amino acids in feed
Ion exchange chromatography
1 Scope
This standard specifies the ion exchange chromatography method for the determination of sulfur-containing amino acids (methionine, cystine and cysteine) in feed.
This standard applies to the determination of sulfur-containing amino acids in feed ingredients, compound feeds, concentrated feeds and concentrate supplements.
The limit of quantification of cystine (containing cysteine) and methionine was 0.01%.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
Determination of water-soluble chloride in GB/T 6439 feed
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 14699.1 Feed sampling
Preparation of GB/T 20195 animal feed samples
JJG1064 Amino Acid Analyzer
3 Principle
The sulfur-containing amino acids in the feed are hydrolyzed by performic acid and hydrolyzed to form sulfoalanine and methionine sulfone by ion exchange chromatography.
Separation by method, post-column derivatization of ninhydrin, spectrophotometric detection, external standard method for quantification.
4 reagents or materials
The reagents used were of analytical grade unless otherwise stated.
4.1 Water. It should meet the requirements of Class I water use in GB/T 6682.
4.2 Hydrochloric acid. excellent grade pure.
4.3 Formic acid. The concentration is 88%.
4.4 Sodium hydroxide. excellent grade.
4.5 Formic acid solution I. Mix hydrogen peroxide with formic acid =1, and add phenol in a ratio of 5 mg per ml, and let it sit at room temperature.
Set for 1 h, cool in an ice water bath for 30 min. Available now.
4.6 Performic acid solution II. Silver nitrate was added in a ratio of 3 mg per ml to the formic acid solution I (4.5).
4.7 Formic acid solution III. Add appropriate amount of silver nitrate to the formic acid solution I (4.5), the amount of addition per ml is calculated according to formula (1).
mR ≥1.454×m×CN (1)
In the formula.
mR---the amount of silver nitrate per milliliter of percarboxylic acid, the unit is milligrams (mg);
CN---Sodium chloride content in the sample, %;
m --- sample mass in milligrams (mg).
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