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GB/T 14643.5-2009: Examination of bacteria and algae in industrial circulating cooling water -- Part 5: Examination of sulfate-reducing bacteria -- MPN test
Status: Valid GB/T 14643.5: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 14643.5-2009 | English | 279 |
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Examination of bacteria and algae in industrial circulating cooling water -- Part 5: Examination of sulfate-reducing bacteria -- MPN test
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GB/T 14643.5-2009
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| GB/T 14643.5-1993 | English | 279 |
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Industrial circulating cooling water sulfate--Reducing Bacteria--MPN test
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GB/T 14643.5-1993
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Basic data | Standard ID | GB/T 14643.5-2009 (GB/T14643.5-2009) | | Description (Translated English) | Examination of bacteria and algae in industrial circulating cooling water -- Part 5: Examination of sulfate-reducing bacteria -- MPN test | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G76 | | Classification of International Standard | 71.040.40 | | Word Count Estimation | 12,192 | | Date of Issue | 2009-05-18 | | Date of Implementation | 2010-02-01 | | Older Standard (superseded by this standard) | GB/T 14643.5-1993 | | Quoted Standard | GB/T 603; GB/T 6682 | | Regulation (derived from) | PRC National Standard Approval Announcement 2009 No.6 (Total No.146) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method for industrial circulating cooling water Determination of sulfate -reducing bacteria. This section applies to the determination of industrial circulating cooling water sulfate-reducing bacteria. Also applies to raw water, domestic water and slime determination of sulfate -reducing bacteria. | GB/T 14643.5-2009: Examination of bacteria and algae in industrial circulating cooling water -- Part 5: Examination of sulfate-reducing bacteria -- MPN test
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Examination of bacteria and algae in industrial circulating cooling water Part 5. Examination of sulfate-reducing bacteria MPN test.
ICS 71.040.40
G76
National Standards of People's Republic of China
Replacing GB/T 14643.5-1993
Industrial circulating cooling water - Determination of bacteria and algae
Part 5. Determination of sulfate-reducing bacteria
MPN method
Posted 2009-05-18
2010-02-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
GB/T 14643 "Determination of industrial circulating cooling water bacteria and algae" is divided into the following sections.
--- Part 1. Determination of slime-forming bacteria plate count
--- Part 2. Determination of soil bacteria plate count
--- Part 3. slime fungi plate count
--- Part 4. Soil fungi plate count
--- Part 5. Determination of sulfate-reducing bacteria MPN method
--- Part 6. Determination of iron bacteria MPN method
This section GB/T 14643 Part 5.
This Part replaces GB/T 14643.5-1993 "Industrial circulating cooling water - Determination of sulfate-reducing bacteria MPN method."
This portion of the GB/T 14643.5-1993 compared to no change in technical content, but the text structure and text of the
modify.
This section of the Appendix A, Appendix B, Appendix C is a normative appendix.
This part is proposed by the China Petroleum and Chemical Industry Association.
This part of the National Standardization Technical Committee of Chemical Water Treatment Chemicals Branch (SAC/TC63/SC5) centralized.
This section is responsible for drafting unit. CNOOC Tianjin Chemical Research & Design Institute, Tianjin Pacific Technology Co., Ltd.
The main drafters of this section. Zhuzhuan Jun, Zhang Quan, Shaohong Qian, Li Lin, Bai Ying.
This section was first released in 1993.
Industrial circulating cooling water - Determination of bacteria and algae
Part 5. Determination of sulfate-reducing bacteria
MPN method
1 Scope
GB/T 14643 provisions of this part of the determination of industrial circulating cooling water sulfate-reducing bacteria.
This section applies to industrial circulating cooling water - Determination of sulfate-reducing bacteria, but also for the raw water, domestic water and slime also Sulfate
Determination of the original bacteria.
2 Normative references
The following documents contain provisions which, through GB/T 14643 reference in this text, constitute provisions of this part. For dated reference documents
Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section
Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this
section.
GB/T 603 chemical reagent test methods in the formulation and preparation of products (GB/T 603-2002, ISO 6353-1 with. 1982,
NEQ)
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
3 Method summary
Law multi-tube fermentation technique, (29 ± 1) ℃ cultured 21d, if you produce a black inner tube and precipitated with hydrogen sulfide odor,
It indicates a positive reaction, the MPN technique being tested samples of sulfate-reducing bacteria counts.
4 Reagents and materials
The reagents used in this section, unless otherwise specified, shall use analytical reagent and in line with GB/T 6682 stipulated three of water.
Test preparation and the desired products, did not indicate when the other requirements, according to GB /603 provisions of the preparation T.
4.1 dipotassium hydrogen.
4.2 ammonium chloride.
4.3 sodium sulfate.
4.4 calcium chloride.
4.5 magnesium sulfate.
4.6 sodium lactate.
4.7 yeast extract. biochemical reagents.
4.8 ferrous ammonium sulfate.
4.9 Vitamin C.
4.10 sodium chloride.
4.11 Sodium hydroxide solution. 40g/L.
4.12 hydrochloric acid solution. 1 + 11.
4.13 sodium thiosulfate.
4.14 ethanol. 75% (volume fraction).
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