Path: Home > GB > Page22 > GB 1903.76-2025 Home > Standard_List > GB > Page22 > GB 1903.76-2025 GB 1903.76-2025 PDF English
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB 1903.76-2025 | English | 125 |
Add to Cart
|
0-9 seconds. Auto-delivery
|
National food safety standard - Nutrition fortifier - Hematin
| Valid |
Excerpted PDFs (Download full copy in 9 seconds upon purchase)PDF Preview: GB 1903.76-2025
GB 1903.76-2025: National food safety standard - Nutrition fortifier - Hematin---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB1903.76-2025
GB
NATIONAL STANDARD OF THE
PEOPLE'S REPUBLIC OF CHINA
National food safety standard - Food nutrition fortifier
Heme iron
Issued on: SEPTEMBER 2, 2025
Implemented on: MARCH 2, 2026
Issued by. National Health Commission of the People's Republic of China;
State Administration for Market Regulation.
Table of Contents
1 Scope... 3
2 Chemical name, molecular formula, structural formula, and relative molecular mass
... 3
3 Technical requirements... 3
Appendix A Test methods... 5
Appendix B Liquid chromatogram... 9
National food safety standard - Food nutrition fortifier
Heme iron
1 Scope
This standard applies to heme iron, a food nutrition fortifier, prepared from animal
blood that has passed inspection and quarantine or from blood cells obtained through
centrifugation, through enzymatic hydrolysis, separation, and drying.
2 Chemical name, molecular formula, structural formula, and
relative molecular mass
2.1 Chemical name
Heme iron
2.2 Molecular formula
C34H32FeN4O4
2.3 Structural formula
2.4 Relative molecular mass
616.499 (based on the international relative atomic mass in 2022).
3 Technical requirements
3.1 Sensory requirements
Sensory requirements shall comply with the provisions of Table 1.
Appendix A
Test methods
A.1 General provisions
Unless otherwise specified, the reagents and water used in the test methods of this
standard refer to analytical grade reagents and Grade III water as specified in GB/T
6682.In the test, the solutions used, unless the solvent used for preparation is specified,
all refer to aqueous solutions.
A.2 Identification test
A.2.1 Reagents and materials
A.2.1.1 Sodium hydroxide.
A.2.1.2 Pyridine.
A.2.1.3 Sodium hyposulfite.
A.2.1.4 Nitric acid. chromatographically pure.
A.2.1.5 Ammonia water.
A.2.1.6 1 mol/L sodium hydroxide solution. Weigh 40.0 g of sodium hydroxide,
dissolve it in carbon dioxide-free water, and bring the volume to 1 L.
A.2.1.7 Pyridine sodium hydroxide solution. Take 100 mL of pyridine, add 30 mL of 1
mol/L sodium hydroxide solution, and dilute with water to 300 mL.
A.2.2 Instruments and equipment
A.2.2.1 Analytical balance. The sensitivity is 0.0001 g.
A.2.2.2 Ultraviolet spectrophotometer.
A.2.3 Identification method
A.2.3.1 Color generation test
A.2.3.1.1 Weigh 0.01 g (accurate to 0.0001 g) of heme iron sample, dissolve it in 50
mL of pyridine sodium hydroxide solution, take 5 mL, and add 15 mg of sodium
hyposulfite. The solution shall turn red.
A.2.3.1.2 Weigh 0.01 g (accurate to 0.0001 g) of heme iron sample into a 100 mL beaker,
add 5 mL of nitric acid, and heat; the solution shall turn yellow. After cooling, alkalize
with ammonia water, then the solution shall turn orange-yellow.
A.2.3.2 Ultraviolet spectroscopy
Weigh 0.01 g (accurate to 0.0001 g) of heme iron sample and dissolve it in 50 mL of
pyridine sodium hydroxide solution. The sample shows maximum absorption at a
wavelength of 399 nm in the ultraviolet light scanning spectrum.
A.3 Determination of heme iron (C34H32FeN4O4) content
A.3.1 Method summary
Heme iron in the sample is dissolved in pyridine-sodium hydroxide, separated by liquid
chromatography, detected by an ultraviolet or diode array detector, qualitatively
determined by retention time, and quantitatively determined by the external standard
method of peak area.
A.3.2 Reagents and materials
Unless otherwise specified, all reagents used are of analytical grade, and the water is
Grade I water as specified in GB/T 6682.
A.3.2.1 Methanol. chromatographically pure.
A.3.2.2 Formic acid. chromatographically pure.
A.3.2.3 Sodium hydroxide.
A.3.2.4 Pyridine.
A.3.2.5 1 mol/L sodium hydroxide solution. Weigh 40.0 g of sodium hydroxide,
dissolve it in carbon dioxide-free water, and bring the volume to 1 L.
A.3.2.6 Pyridine sodium hydroxide solution. Take 100 mL of pyridine, add 30 mL of 1
mol/L sodium hydroxide solution, and dilute with water to 300 mL.
A.3.2.7 Hematin (C34H33FeN4O5, CAS No.. 15489-90-4) standard. Content ≥98.0%.
A.3.2.8 Hematin standard stock solution. Accurately weigh 0.1028 g of hematin dried
at 100 ℃ for 3 h, dissolve it in pyridine sodium hydroxide solution (A.3.2.6), and dilute
to 100 mL. This solution is equivalent to 1 mg/mL of heme iron. Store in a brown
reagent bottle at 0 ℃~4 ℃ protected from light. The shelf life is 7 days.
A.3.2.9 Filter membrane. 0.45 μm, aqueous phase.
A.3.3 Instruments and equipment
A.3.3.1 High-performance liquid chromatograph. It is equipped with an ultraviolet
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
Price & DeliveryUS$125.00 · In stock · Download in 9 secondsGB 1903.76-2025: National food safety standard - Nutrition fortifier - Hematin Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid
Tips & Frequently Asked QuestionsQuestion 1: How long will the true-PDF of English version of GB 1903.76-2025 be delivered?Answer: The full copy PDF of English version of GB 1903.76-2025 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice. Question 2: Can I share the purchased PDF of GB 1903.76-2025_English with my colleagues?Answer: Yes. The purchased PDF of GB 1903.76-2025_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. www.ChineseStandard.us -- GB 1903.76-2025 -- Click this link and select your country/currency to pay, the exact amount in your currency will be printed on the invoice. Full PDF will also be downloaded/emailed in 9 seconds. How to buy and download a true PDF of English version of GB 1903.76-2025?A step-by-step guide to download PDF of GB 1903.76-2025_EnglishStep 1: Visit website https://www.ChineseStandard.net (Pay in USD), or https://www.ChineseStandard.us (Pay in any currencies such as Euro, KRW, JPY, AUD).
Step 2: Search keyword "GB 1903.76-2025".
Step 3: Click "Add to Cart". If multiple PDFs are required, repeat steps 2 and 3 to add up to 12 PDFs to cart.
Step 4: Select payment option (Via payment agents Stripe or PayPal).
Step 5: Customize Tax Invoice -- Fill up your email etc.
Step 6: Click "Checkout".
Step 7: Make payment by credit card, PayPal, Google Pay etc. After the payment is completed and in 9 seconds, you will receive 2 emails attached with the purchased PDFs and PDF-invoice, respectively.
Step 8: Optional -- Go to download PDF.
Step 9: Optional -- Click Open/Download PDF to download PDFs and invoice.
See screenshots for above steps: Steps 1~3 Steps 4~6 Step 7 Step 8 Step 9
|