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GB 1903.11-2015 English PDF

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GB 1903.11-2015: National food safety standard -- Food Nutrition Enhancer -- Zinc lactate
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GB 1903.11-2015English169 Add to Cart 3 days [Need to translate] National food safety standard -- Food Nutrition Enhancer -- Zinc lactate Valid GB 1903.11-2015

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Basic data

Standard ID GB 1903.11-2015 (GB1903.11-2015)
Description (Translated English) National food safety standard -- Food Nutrition Enhancer -- Zinc lactate
Sector / Industry National Standard
Classification of Chinese Standard X40
Classification of International Standard 67.220.20
Word Count Estimation 8,828
Date of Issue 2015-11-13
Date of Implementation 2016-05-13
Regulation (derived from) National Health and Family Planning Commission Announcement No
Issuing agency(ies) National Health and Family Planning Commission of the People's Republic of China
Summary This standard applies to the production of lactic acid and zinc oxide fermentation as the main raw material, the chemical reaction, processing, purification and food nutrition fortifier zinc lactate.

GB 1903.11-2015: National food safety standard -- Food Nutrition Enhancer -- Zinc lactate

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(National food safety standards of food nutrition fortifier zinc lactate) National Standards of People's Republic of China National Food Safety Standard Food nutrition fortifier zinc lactate Issued on. 2015-11-13 2016-05-13 implementation People's Republic of China National Health and Family Planning Commission released National Food Safety Standard Food nutrition fortifier zinc lactate

1 Scope

This standard applies to lactic acid produced by fermentation and zinc oxide as the main raw material, chemical reaction, processing, purification and prepared foods business Yang enhancer zinc lactate. 2 chemical name, molecular formula, molecular mass and structural formula 2.1 Chemical Name Zinc 2-hydroxy-propyl Formula 2.2 C6H10O6Zn · nH2O (n = 2 or 3) 2.3 formula 2.4 relative molecular mass 279.55 (dihydrate) (according to 2012 international relative atomic mass) 297.57 (trihydrate) (according to 2012 international relative atomic mass)

3 Technical requirements

3.1 Sensory requirements Sensory requirements shall comply with the requirements of Table 1. Table 1 Sensory requirements Project requires test methods Color White State powder Take the right amount of sample is placed in a clean, dry transparent containers, observe its color in natural light, status 3.2 Physical and Chemical Indicators Physical and chemical indicators should be consistent with the provisions of Table 2. Table 2. Physical and chemical indicators project index L- lactic acid zinc zinc lactate Testing method Total zinc lactate (C6H10O6Zn) content (dry basis), w /% ≥ 98.0 Appendix A A.3 The percentage of the total zinc L- lactic acid zinc content /% ≥ 96.0 - Appendix A A.4 Loss on drying, w /% Dihydrate ≤ 14 Trihydrate ≤ 18.5 Appendix A A.5 pH (1% aqueous solution) 5.0 ~ 7.0 GB/T 9724 Lead (Pb)/(mg/kg) ≤ 3.0 GB 5009.12 Total arsenic (As)/(mg/kg) ≤ 2.0 GB 5009.11 Cadmium (Cd)/(mg/kg) ≤ 2.0 GB 5009.15

Appendix A

Testing method A.1 General Provisions This standard reagents and water, did not indicate when the other requirements, refer to the three water analytical reagent and GB/T 6682 stipulated. Standard solutions used in the tests, the determination of impurities standard solution preparation and its products, did not indicate when the other requirements, according to GB/T 601, GB/T 602, the provisions of the preparation of GB/T 603's. Solution was used in the tests did not indicate what is formulated with solvent, it refers to an aqueous solution. A.2 Identification Test A.2.1 Reagents and materials A.2.1.1 ethanol. A.2.1.2 hydrochloric acid solution. 14. A.2.1.3 sulfuric acid solution. 119. A.2.1.4 potassium permanganate solution. 0.1mol/L. A.2.1.5 morpholino solution. 14. A.2.1.6 sodium nitroprusside solution. 50g/L. A.2.1.7 potassium ferrocyanide solution. 100g/L, prepared just before use. A.2.2 Identification method A.2.2.1 solubility Room temperature soluble in water, slightly soluble in ethanol. A.2.2.2 Identification of zinc salt Weigh about 0.2g sample was dissolved in 10mL water, 2mL potassium ferrocyanide solution was added freshly prepared, i.e., the white precipitate. Shen separation Lake, hydrochloric acid solution was added to the precipitate, the insoluble precipitate. A.2.2.3 Identification of lactate Weigh about 0.5g sample was dissolved in 10mL water, mix a solution of sulfuric acid was added 2mL, plus 2mL potassium permanganate solution, heated, that is made The smell of acetaldehyde. A mixture of morpholino solution and sodium nitroprusside solution identification acetaldehyde gas using an equal volume of moistened filter Paper, filter paper in contact with the gas phase was blue. A.3 Determination of total zinc lactate (C6H10O6Zn) content (dry basis) of A.3.1 Reagents and materials A.3.1.1 ammonia - ammonium chloride buffer solution (pH≈10). A.3.1.2 disodium EDTA standard titration solution. c (EDTA) = 0.05mol/L. A.3.1.3 chrome black T indicator. A.3.2 Analysis step Weigh about 0.3g dried A (A.5.1), accurate to 0.0001g, dissolved in 30mL of water (necessary, to add heat), put to room temperature, 10mL ammonia - ammonium chloride buffer solution with a little chrome black T indicator, using standard titration Disodium EDTA titration solution to the solution from purple Pure red to blue as the end point. At the same time a blank test. A.3.3 Calculation Results Total zinc lactate (C6H10O6Zn) content (dry basis) of the mass fraction w1, according to equation (A.1) Calculated. w1 = V-V0 1000 × c × M m1 × 100% (A.1) Where. V --- sample consumption volume of disodium EDTA standard titration solution, in milliliters (mL); Blank consumption volume V0 --- disodium EDTA standard titration solution, in milliliters (mL); 1000 --- conversion factor; Concentration c --- disodium edetate standard titration solution, expressed in moles per liter (mol/L); --- The M zinc lactate molar mass in grams per mole (g/mol), [M (C6H10O6Zn) = 243]; m1 --- sample mass, in grams (g). Parallel determination results take twice the arithmetic mean prevail. Two parallel determination results is not more than 0.3% absolute difference. The percentage content of zinc lactate determination of total zinc lactate A.4 L- A.4.1 Method summary By high performance liquid chromatography, under the selected operating conditions, the sample solution through the column L- zinc lactate and zinc lactate component sub-D- Away with a UV detector and area normalization with quantitative calculation of the total percentage of L- lactic acid zinc zinc content. A.4.2 Reagents and materials A.4.2.1 copper sulfate pentahydrate (CuSO4 · 5H2O). A.4.2.2 Water. GB/T 6682 specified in a water. A.4.2.3 copper sulfate pentahydrate (CuSO4 · 5H2O) solution. 0.001mol/L. Weigh 0.25g copper sulfate pentahydrate (CuSO4 · 5H2O), Dissolved in water and dilute to 1L, shake spare. A.4.3 Instruments and Equipment High performance liquid chromatograph. equipped with UV detector. A.4.4 reference chromatographic conditions A.4.4.1 Column. with an optically active ligand exchange-type stationary phase coated on octadecylsilane bonded silica silica filler chiral Column, 150mm × 4.6mm, particle size 5μm; or other equivalent column. A.4.4.2 Mobile phase. 0.001mol/L of copper sulfate pentahydrate (CuSO4 · 5H2O) solution. A.4.4.3 mobile phase flow rate. 1.0mL/min. A.4.4.4 detection wavelength. 254nm. A.4.4.5 Injection volume. 5μL. A.4.4.6 Column temperature. 35 ℃. A.4.5 Analysis step A.4.5.1 Preparation of sample solution Weigh about 0.15g sample, accurate to 0.0001g, after dissolved by mobile phase, transferred to a 100mL volumetric flask, add the mobile phase to volume Scale, shake spare. L- lactic acid zinc reference chromatogram of the sample solution in Appendix B. A.4.5.2 Determination In reference chromatographic conditions (A.4.4.), The sample solution was subjected to chromatographic analysis to determine a sample solution chromatogram D- and L- lactate, zinc lactate Zinc corresponding peak (retention time as L- zinc lactate 20.8min, D- zinc lactate retention time 27.4min). Record color sample solution Spectrum L- and D- lactic acid zinc component of the zinc component of peak area AL and AD, by area normalized quantitative calculation of the total zinc lactate L- The percentage content of zinc lactate. A.4.6 Calculation Results L- zinc lactate percentage of total zinc lactate concentration w2, according to equation (A.2) Calculated. w2 = AL AL AD × 100% (A.2) Where. AL --- sample solution chromatogram peak area L- lactic acid zinc component; AD --- sample solution chromatogram peak area D- lactic acid zinc component. Parallel determination results take twice the arithmetic mean prevail. Two parallel determination results is not more than 0.3% absolute difference. A.5 Determination of loss on drying A.5.1 Analysis step Weigh about 1.5g sample, accurate to 0.0001g, placed in a pre dried to constant weight at 120 ℃ ± 2 ℃ weighing bottle, paved thickness Layer of 3mm or less. At 120 ℃ ± 2 ℃ constant temperature drying oven 4h, placed in a desiccator to cool 30min weighing. Reserved section Dried (this is dried A) for the determination of total zinc lactate content (A.3). A.5.2 Calculation Results Loss on drying mass fraction w3, according to equation (A.3) Calculated. w3 = m2-m3 m2 × 100% (A.3) Where. M2 --- dried before the mass of the sample, in grams (g); Quality, unit m3 --- after drying the sample in grams (g). Parallel determination results take twice the arithmetic mean prevail. Two parallel determination results is not more than 0.2% absolute difference.

Appendix B

Zinc lactate reference chromatogram of the sample solution Zinc lactate solution reference sample chromatogram is shown in Figure B.1. Figure B.1 zinc lactate reference chromatogram of the sample solution 5102- 11􀏕 3091

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