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US$119.00 · In stock Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB 1886.225-2016: National Food Safety Standard -- Food Additives -- Ethoxyquin Status: Valid
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National Food Safety Standard -- Food Additives -- Ethoxyquin
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GB 1886.225-2016
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Basic data | Standard ID | GB 1886.225-2016 (GB1886.225-2016) | | Description (Translated English) | National Food Safety Standard -- Food Additives -- Ethoxyquin | | Sector / Industry | National Standard | | Classification of Chinese Standard | X40 | | Word Count Estimation | 6,683 | | Date of Issue | 2016-08-31 | | Date of Implementation | 2017-01-01 | | Regulation (derived from) | Announcement of the State Administration of Public Health and Family Planning 2016 No.11 | | Issuing agency(ies) | National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration |
GB 1886.225-2016: National Food Safety Standard -- Food Additives -- Ethoxyquin---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Food safety national standard - Food additive - Ethoxyquin)
National Standards of People's Republic of China
National Food Safety Standard
Food additive Ethoxyquin
Published 2016-08-31
2017-01-01 implementation
People's Republic of China
National Health and Family Planning Commission issued
National Food Safety Standard
Food additive Ethoxyquin
1 Scope
This standard applies to phenetidine and acetone as raw materials, the resulting catalyst under the reaction conditions and heating food additives ethoxyethyl
Quinoline base.
2 chemical name, formula, formula and molecular mass
2.1 Chemical Name
6-ethoxy-1,2-dihydro-2,2,4-trimethyl quinoline
2.2 Structure
Formula 2.3
C14H19NO
2.4 Molecular Weight
217.31 (according to 2011 international relative atomic mass)
3 Technical requirements
3.1 Sensory requirements
Sensory requirements shall comply with the requirements in Table 1.
Table 1 Sensory requirements
Project requires test methods
Color
Light yellow to amber, light in a long time, the air
After standing gradually turned dark brown
Viscous liquid state
Proper amount of sample is placed in clean, dry cuvette, in natural light
The observed color and state
3.2 Physical and Chemical Indicators
Physical and chemical indicators should be consistent with the provisions of Table 2.
Table 2. Physical and chemical indicators
Item Index Test Method
Ethoxyquin, w /% ≥ 98.0 Appendix A A.3
Phenetidine, w /% ≤ 0.2 Appendix A A.4
Lead (Pb)/(mg/kg) ≤ 2.0 GB 5009.12
Arsenic (As)/(mg/kg) ≤ 2.0 GB 5009.76
Appendix A
Testing method
A.1 General Provisions
This standard reagents and water, in the absence of other requirements specified, refer to three analytical reagent water and GB/T 6682 provisions. test
Test used in the standard solution, the standard solution, the determination of impurities, products and preparations, in the absence of other requirements specified, are by GB/T 601,
GB/T 602 and GB/T 603 Preparation predetermined. The test does not indicate when the solution which is formulated with a solvent, refer to the aqueous solution.
A.2 Identification Test
The sample was dissolved in 10mL of acetonitrile 1mg and observed under UV light, can show strong fluorescence.
Determination of ethoxyquin A.3
A.3.1 Reagents and materials
A.3.1.1 glacial acetic acid.
A.3.1.2 methyl violet indicator solution. 10g/L. Weigh 1g Methyl Violet, dissolved in an appropriate amount of glacial acetic acid, transferred to 100mL flask with ice
Acid volume.
Perchloric acid standard titration solution A.3.1.3. c (HClO4) = 0.1mol/L.
A.3.1.4 acetic anhydride.
A.3.2 Analysis step
Weigh about 0.2g sample, accurate to 0.0001g, dried in a clean Erlenmeyer flask was added 50mL acetic acid was dissolved to make ice, plus
Into 1mL of acetic anhydride mixed, add 2 drops of methyl purple indicator solution, titration with perchloric acid standard solution was titrated to green is the end.
At the same time a blank test.
A.3.3 calculation results
Ethoxyquin mass fraction w1, according to formula (A.1) is calculated.
w1 =
(V-V0) × c × M
1000 × m ×
100% (A.1)
Where.
V --- Sample consumption perchloric acid standard titrant volume in milliliters (mL);
--- consumption volume V0 blank test standard solution of perchloric acid in milliliters (mL);
C --- perchloric acid concentration standard titration solution in units of moles per liter (mol/L);
--- M is ethoxyquin molar mass in grams per mole (g/mol) [M (C14H19NO) = 217.31];
1000 --- volume conversion factor;
--- m sample mass, in grams (g).
The arithmetic average of the results of the two replicates is reported results. The absolute difference determination results parallel to not more than 0.4%.
Determination of phenetidine A.4
A.4.1 Method summary
By gas chromatography under the selected operating conditions, the respective components of the sample injector was isolated, detected by a hydrogen flame detector, with external
Standard method.
A.4.2 Reagents and materials
A.4.2.1 ethanol.
A.4.2.2 nitrogen. mass fraction of ≥99.99%.
A.4.2.3 diethyl phthalate internal standard solution. 100μg/mL. Weigh 1g diethyl phthalate, accurate to 0.0001g, to
Dissolving a small amount of ethanol, into a volumetric flask, dilute to ethanol 100mL.
A.4.2.4 of standard aminophenyl ether solution. 10mg/mL. Weigh about 0.05g phenetidine (purity ≥99%), to the nearest
0.0001g, in stoppered glass vial, was added 5mL diethyl phthalate internal standard solution (A.4.2.3) pipetting with a pipette, cover
Cork, shake.
A.4.2.5 sample solution. 60mg/mL. Weigh about 0.3g sample, accurate to 0.0001g, in stoppered glass vial, was added with a pipette
Pipette tube in 5mL diethyl phthalate internal standard solution (A.4.2.3), stoppered, shaken.
A.4.3 Instruments and Equipment
A.4.3.1 gas chromatograph. equipped with hydrogen flame ionization detector.
A.4.3.2 micro-syringe.
A.4.4 column and operating conditions
The standard recommended chromatographic column and operating conditions are shown in Table A.1. Other to achieve the same degree of separation columns and LC operating conditions
Member may also be used.
Table A.1 Recommended chromatographic column and operating conditions
Project Parameters
Column 5% phenyl - methyl polysiloxane
Inside diameter × length × thickness of column column 30m × 0.32mm × 0.25μm
Column temperature/℃
Initial temperature 60 ℃, temperature rising rate of 10.0 ℃/min was raised to 200 ℃, holding 6min,
At a rate of 20 ℃/min raised to 280 ℃, kept 10min
Gasification chamber temperature/℃ 240
Detector temperature/℃ 280
Nitrogen carrier gas
Flow rate/(mL/min) 40
A.4.5 Analysis step
After the instrument is stable, the number of injection needles standard solution, until the adjacent base stable peak area ratio of the two needles, analyzed in the following order.
a) phenetidine standard solution (A.4.2.4);
b) the sample solution (A.4.2.5);
c) sample solution (A.4.2.5);
d) phenetidine standard solution (A.4.2.4).
The injection volume are 1μL.
A.4.6 calculation results
The order a) → b) → c) → d) of the injection, each injection peak area ratio f calculated according to the formula (A.2).
f =
S1
S2
(A.2)
Where.
--- peak area Sl aminophenyl ether;
--- peak area S2 within the standard.
According to formula (A.2) obtained fa and fd, fb, and fc are averaged, to obtain f1 and f2.
Phenetidine quality score w2, calculated according to formula (A.3).
w2 =
f1 × m1 × w
f2 × m2 ×
100% (A.3)
Where.
F1 --- phenetidine standard solution averaged ratio of peak area of phenetidine and the internal standard;
M1 --- phenetidine quality standards, the unit is gram (G);
W --- mass fraction of phenetidine standard,%;
F2 --- averaged sample solution aminophenyl ether ratio of peak area of the internal standard;
--- M2 sample mass, in grams (g).
The arithmetic average of the results of the two replicates is reported results. The absolute difference determination results is not larger than the two parallel assay
6% of the arithmetic mean values.
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