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GB 1886.115-2015 English PDF

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GB 1886.115-2015: National Food Safety Standard -- Food Additives -- Red beans
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Basic data

Standard ID GB 1886.115-2015 (GB1886.115-2015)
Description (Translated English) National Food Safety Standard -- Food Additives -- Red beans
Sector / Industry National Standard
Classification of Chinese Standard X40
Word Count Estimation 8,844
Date of Issue 2015-09-22
Date of Implementation 2016-03-22
Regulation (derived from) PRC National Health and Family Planning Commission 2015 No.8
Issuing agency(ies) National Health and Family Planning Commission of the People's Republic of China

GB 1886.115-2015: National Food Safety Standard -- Food Additives -- Red beans

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(National food safety standards for food additives red beans) National Standards of People's Republic of China National Food Safety Standard Food Additives red beans Issued on. 2015-09-22 2016-03-22 implementation People's Republic of China National Health and Family Planning Commission released National Food Safety Standard Food Additives red beans

1 Scope

This standard applies to beans (GlyeinesojaSieb.etZucc) seed coat as raw material, extracted with dilute ethanol extract, concentrated by Shrinking, drying food additive red beans. 2 chemical name, molecular formula, molecular mass and structural formula 2.1 Chemical Name Cyanidin -3- galactosidase Formula 2.2 C21H21O11 2.3 formula 2.4 relative molecular mass 449.38 (according to 2007 international relative atomic mass)

3 Technical requirements

3.1 Sensory requirements Sensory requirements shall comply with the requirements of Table 1. Table 1 Sensory requirements Project requires test methods Color purple State powder Take appropriate sample in a clean, dry colorimetric Tube, under natural light, visually observed with color Chak and state 3.2 Physical and Chemical Indicators Physical and chemical indicators should be consistent with the provisions of Table 2. Table 2. Physical and chemical indicators Item Index Test Method pH 3.5 ~ 4.5 in Appendix A A.3 Absorbance E1 m (525 ± 5) nm ≥ 20.0 Appendix A A.4 Loss on drying, w /% ≤ 5.0 A.5 in Appendix A Residue on ignition, w /% ≤ 11.0 A.6 in Appendix A Lead (Pb)/(mg/kg) ≤ 3.0 GB 5009.75 Arsenic (As)/(mg/kg) ≤ 2.0 GB 5009.76 Note. The red beans commercialization of products conforming to this standard shall be red beans as raw material, add edible dextrin, antioxidants and other accessories made of.

Appendix A

Testing method A.1 General Provisions This standard reagents and water, did not indicate when the other requirements, refer to analytical reagent and third grade water GB/T 6682 regulations. Standard solutions used in the tests, impurity standard solution, preparations and products, did not indicate when the other requirements, according to GB/T 601, GB/T 602 And the provisions of GB/T 603 formulation. Solution was used in the tests did not indicate what is formulated with solvent, it refers to an aqueous solution. A.2 Identification Test A.2.1 solubility A.2.1.1 soluble in water and ethanol, transparent and no precipitation. Insoluble in non-polar solvent ether, acetone and the like. A.2.1.2 When an aqueous solution of neutral purple clear solution; when the acidic aqueous solution is a transparent cherry red; blue-violet alkaline aqueous solution Color transparent solution. A.2.2 color reaction A.2.2.1 Take a little water sample drop on filter paper, placed in dry ammonia vapor, the sample into the blue. Immediately placed under ultraviolet light Concept Police, with light blue fluorescence. A.2.2.2 Take a little water sample drop on filter paper, dry solution of concentrated sulfuric acid was spotted at 1 drop of orange-yellow spots. A.2.2.3 The sample was dissolved in 95% ethanol, and then taken in a test tube 1mL, plus magnesium, plus the number of concentrated hydrochloric acid drops immediately visible by the red liquid Faded pink. A.2.3 absorption maxima A.2.3.1 Reagents and materials A.2.3.1.1 ethanol. A.2.3.1.2 hydrochloric acid. A.2.3.1.3 Ethanol - hydrochloric acid solution. volume fraction of 40% ethanol solution, concentrated hydrochloric acid adjusted to pH 3.0. A.2.3.2 analysis step Take 0.15g sample with ethanol - hydrochloric acid solution volume to 100mL, which take 10mL, then ethanol - hydrochloric acid solution volume to 100mL, the solution in the absorption peak wavelength at 525nm ± 5nm. A.2.4 paper chromatography A.2.4.1 instruments and equipment A.2.4.1.1 chromatography tank. A.2.4.1.2 capillaries. A.2.4.1.3 filter. 15cm × 7cm. A.2.4.2 Reagents and materials A.2.4.2.1 butanol. A.2.4.2.2 glacial acetic acid. A.2.4.2.3 developing solvent. n-butanol. acetic acid. water = 4.1.2 (volume ratio). A.2.4.3 analysis step The developer added to the dish, which is placed in chromatography tank. Chromatography tank to make room for the agent fully saturated steam. At a distance of about 1.5cm at the thin end of the filter paper pencil scratching a line, at this point the line of red pigment black beans 1% ethanol solution of the sample 5μL ~ 10μL, spot diameter is not more than 2mm, and air dried. Add chromatography cylinder at a temperature of 16 ℃ ± 2 ℃ condition upward swing. Flow to be compatible Agent to two-thirds of the filter paper, remove the filter paper with a pencil to draw solvent front and the spot position, shape. A.2.4.4 Calculation Results Rf Rf, according to equation (A.1) Calculated. Rf = (A.1) Where. b --- the origin to the center of the spot component distance in centimeters (cm); A --- the origin to the solvent front distance in centimeters (cm). Results. Rf = 0.6 ± 0.05. The results parallel arithmetic mean of the measurement results shall prevail. A.3 pH measurement A.3.1 Instruments and Equipment PH meter. A.3.2 Analysis step The sample was dubbed in 1% aqueous solution, with a pH meter. The results parallel arithmetic mean of the measurement results shall prevail. A.4 Determination of absorbance A.4.1 Instruments and Equipment Spectrophotometer. A.4.2 Reagents and materials A.4.2.1 ethanol. A.4.2.2 hydrochloric acid. A.4.2.3 Ethanol - hydrochloric acid solution. volume fraction of 40% ethanol solution, concentrated hydrochloric acid adjusted to pH 3.0. A.4.3 Analysis step Weigh the sample 0.15g (accurate to 0.0001g), with ethanol - hydrochloric acid solution volume to 100mL. Reap 10mL, followed by ethanol - salt Acid solution volume to 100mL. Remove the dilution in 1cm cuvette using a spectrophotometer at a wavelength of 525nm ± 5nm Department to B Alcohol - hydrochloric acid solution as the reference solution, measure the absorbance (E1 m (525 ± 5) nm). A.4.4 Calculation Results Absorbance E1 m (525 ± 5) nm, according to equation (A.2) Calculated. E1 m (525 ± 5) nm = A × f m1 × (A.2) Where. A --- absorbance; f --- dilution factor; M1 --- the quality of the sample, in grams (g); 100 --- conversion factor. The results parallel arithmetic mean of the measurement results shall prevail. A.5 Determination of loss on drying A.5.1 Instruments and Equipment A.5.1.1 weighing bottle. A.5.1.2 oven. A.5.2 Analysis step Weigh the sample 1g (accurate to 0.002g), constant weight weighing bottle is placed at 105 ℃ ± 1 ℃ oven for about 4h, cooling, weighing, to Constant weight. A.5.3 Calculation Results Loss on drying of the sample mass fraction w1, according to equation (A.3) Calculated. w1 = m2-m3 m4 × 100% (A.3) Where. m2 --- weighing bottle plus the quality of the sample, in grams (g); m3 --- dried weighing bottle plus the quality of the sample, in grams (g); m4 --- sample mass, in grams (g). The results parallel arithmetic mean of the measurement results shall prevail. A.6 Determination of residue on ignition A.6.1 Instruments and Equipment A.6.1.1 crucible. A.6.1.2 high-temperature furnace. A.6.2 Analysis step Accurately weighed sample 3g (accurate to 0.01g), constant weight has been placed in a crucible, first carbonization (about 300 ℃), and then transferred to 800 ℃ ± 25 ℃ high temperature burning furnace to constant weight. A.6.3 Calculation Results Residue on ignition of the sample mass fraction w2, according to equation (A.4) Calculated. w2 = m5 m6 × 100% (A.4) Where. Quality m5 --- residue on ignition in grams (g); m6 --- sample mass, in grams (g). The results parallel arithmetic mean of the measurement results shall prevail. 5102- 511􀏕 6881

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