HOME   Cart(0)   Quotation   About-Us Policy PDFs Standard-List
www.ChineseStandard.net Database: 189760 (18 Oct 2025)

GA/T 842-2019 English PDF

US$229.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GA/T 842-2019: Examination methods for ethanol concentration in blood
Status: Valid

GA/T 842: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GA/T 842-2019English229 Add to Cart 3 days [Need to translate] Examination methods for ethanol concentration in blood Valid GA/T 842-2019
GA/T 842-2009English439 Add to Cart 3 days [Need to translate] Analysis method for ethanol concentration in blood Obsolete GA/T 842-2009

PDF similar to GA/T 842-2019


Standard similar to GA/T 842-2019

GB/T 35548   GB/T 35347   JT/T 883   GA/T 488   GA/T 414   GA/T 1370   

Basic data

Standard ID GA/T 842-2019 (GA/T842-2019)
Description (Translated English) Examination methods for ethanol concentration in blood
Sector / Industry Public Security (Police) Industry Standard (Recommended)
Classification of Chinese Standard R86
Classification of International Standard 11.040.20
Word Count Estimation 10,112
Date of Issue 2019
Date of Implementation 2019-05-01
Issuing agency(ies) Ministry of Public Security

GA/T 842-2019: Examination methods for ethanol concentration in blood

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Examination methods for ethanol concentration in blood ICS 11.040.20 R 86 People's Republic of China Public Safety Industry Standard GA Replaces GA/T 842-2009 Test method for blood alcohol content Published by the Ministry of Public Security of the People's Republic of China

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces GA/T 842-2009 "Test method for blood alcohol content". Compared with GA/T 842-2009, The main technical changes are as follows. -Added "normative references" (see Chapter 2); -Revised the requirements for "reagents" (see Chapter 5, Chapter 5 of the.2009 edition); -Deleted the content of "n-propanol" (see Chapter 5 of the.2009 edition); -Added requirements for "analytical balances" (see Chapter 6); -Deleted “PTFE film” (see Chapter 6 of the.2009 edition); -Revised the requirements for "sample preparation" (see 7.1.1, 7.1.1 of the.2009 edition); -The requirements of “Reference Conditions for Headspace Sample Analysis” have been modified (see 7.1.2.2, 7.1.2.2 of the.2009 edition); -Deleted the content of "headspace manual injection" (see 7.1.2.3.2 of the.2009 version); -Revised the requirements for "sample preparation" (see 7.2.1, 7.2.1 of.2009 edition); -The requirements for "quantification" have been modified (see 7.2.3.2, 7.2.3.2 of the.2009 edition); -Revised the requirements for "quantitative results" (see 8.2.2,.2009 version 8.2.2); -The name of “Determination Limit” has been modified (see 8.2.3, 8.2.3 of the.2009 edition); -Removed Appendix A (see Appendix A of the.2009 edition); -Removed Appendix B (see Appendix B of the.2009 edition). This standard was proposed by Tianjin Public Security Traffic Management Bureau. This standard is under the jurisdiction of the National Road Traffic Management Standardization Technical Committee. This standard is drafted by. Tianjin Public Security Traffic Management Bureau. This standard participated in the drafting unit. Shanxi Provincial Public Security Department Traffic Police Corps. The main drafters of this standard. Shen Yuming, Liu Junfang, Cai Hongyan, Zhuang Xuying, Xing Jianyu, Li Dezhuang, Liu Zhao, Yao Chenyu, Li Huaiyu, Yang Kun. The previous versions of the standards replaced by this standard are. --GA/T 842-2009. Test method for blood alcohol content

1 Scope

This standard specifies the headspace gas chromatography test method for blood alcohol content. This standard applies to the qualitative and quantitative analysis of alcohol in the blood of persons during road traffic law enforcement activities.

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this document. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682-2008 Analytical laboratory water specifications and test methods GB 19522 Threshold and test of blood and breath alcohol content of vehicle driver GA/T 122 Toxicology Glossary

3 terms and definitions

The terms and definitions defined in GB 19522 and GA/T 122 apply to this document.

4 Principle

This method uses the volatile nature of alcohol (hereinafter referred to as ethanol), uses tert-butanol as an internal standard, and uses a headspace gas chromatography flame ionization detector. Detection; comparison with ethanol standards in parallel, qualitative by retention time or relative retention time, internal standard method using ethanol The peak area ratio was quantified.

5 reagents

Unless otherwise specified, the reagents used in this standard are chromatographically pure. The test water is secondary water (see GB/T 6682-2008). a) ethanol; b) tert-butanol; c) Ethanol standard working solution. Precisely pipette or weigh an appropriate amount of absolute ethanol standard, and prepare 1000 mg/100 mL ethanol stock with water. Liquid, sealed, refrigerated storage, the use period is 6 months. Dilute the ethanol stock solution by multiples with water to make a series of concentrations used in the test Standard working solution of ethanol, the use period is 3 months; d) tert-butanol internal standard working solution. accurately absorb or weigh the appropriate amount of tert-butanol standard, and prepare 4 mg/100 mL tert-butanol internal standard with water The working solution is sealed and stored under refrigeration for a period of 3 months.

6 Equipment

The headspace gas chromatography test method should have the following equipment. a) Gas chromatograph. equipped with hydrogen flame ionization detector (FID); b) headspace autosampler; c) Analytical balance. Sensitivity 0.1mg; d) precision pipettes; e) sample vials; f) silicone rubber pads; g) aluminum caps; h) Sealing pliers.

7 Operation method

7.1 Qualitative analysis 7.1.1 Sample preparation 7.1.1.1 Case sample preparation Take 0.10 mL of whole blood to be tested and 0.50 mL of tert-butanol internal standard working solution into a sample bottle, cover with a silicone rubber pad, and seal aluminum with sealing forceps. Cap, mix well, to be tested. 7.1.1.2 Preparation of blank samples and added samples Take 1 mg/100 mL ethanol standard working solution 0.10 mL and tert-butanol internal standard working solution 0.50 mL into the sample bottle as the detection limit Add samples, and take another 0.10 mL of blank whole blood and 0.50 mL of tert-butanol internal standard working solution into the sample bottle as a blank sample. As per 7.1.1.1 Method operation, parallel control analysis with case samples. 7.1.2 Detection 7.1.2.1 Reference conditions for gas chromatography detection The reference conditions for gas chromatography detection are shown in Table 1. It can be adjusted according to the actual situation of different brands of instruments and different samples. Table 1 Reference conditions for gas chromatography detection reference condition Column 1) Column temperature Carrier gas flow rate mL/min Detector Detector temperature Inlet temperature DB-ALC1 (30 m × 0.32 mm × 1.8 μm) hair Thin column or equivalent column Flame off Sub-test Tester (FID) 250.200 DB-ALC2 (30 m × 0.32 mm × 1.2μm) hair Thin column or equivalent column PLOT-Q (30 m × 0.53 mm × 20.00 μm) hair Thin column or equivalent column 5 rbowax 20 M/Carbopack (80 ~ 120 Mesh) 2 m × 2 mm packed column or equivalent column 70 20 ~ 40 7.1.2.2 Reference Conditions for Headspace Sample Analysis Reference Conditions for Headspace Autosampler a) Heating box temperature. 65 ℃; 1) DB-ALC1 and DB-ALC2 are suitable examples of commercially available products. This information is given for the convenience of the users of this standard and does not imply that Approved. b) Loop temperature. 105 ℃; c) Transmission line temperature. 110 ℃; d) Heating equilibration time of sample bottle. 10.00 min; e) Sample bottle pressurization time. 0.10 min; f) Filling time of the loop. 0.10 min; g) Loop equilibrium time. 0.05 min; h) Injection time. 1.00 min. 7.1.2.3 Injection Place the prepared sample on the headspace autosampler sample rack, prepare an injection method, and run it. 7.1.3 Records and calculations Record the retention times of t-butanol, ethanol standards, and suspicious ethanol peaks in each sample, and calculate the ethanol relative to t-butanol in each sample Relative retention time value. 7.2 Quantitative analysis 7.2.1 Sample preparation 7.2.1.1 Case sample preparation Take 0.10 mL of the whole blood to be tested and add 0.50 mL of tert-butanol internal standard working solution, the sample preparation is the same as 7.1.1.1. 7.2.1.2 Preparation of calibration curve Configure a series of standard ethanol working solutions. Take a series of 0.10 mL of each standard working solution and t-butanol internal standard working solution 0.50 mL was added to each sample vial. The sample preparation was the same as 7.1.1.1. The concentration of the ethanol standard working solution series used to prepare the calibration curve should be no less than six step concentrations, and the ethanol concentration of the sample to be measured should be in the school Within the linear range of the quasi-curve. 7.2.2 Detection and recording According to 7.1.2, record the peak area values of ethanol and t-butanol in the case samples and the series of standard working solution samples. 7.2.3 Calculation 7.2.3.1 Calibration curve Linear regression of the mass concentration of ethanol using the ratio of the peak area of ethanol to t-butanol in a series of standard working solution samples yields the line Sexual equation. 7.2.3.2 Quantitative According to the peak area values of ethanol and internal standard tert-butanol in the case sample, calculate the ethanol content X in the case sample according to formula (1). X = aY (1) In the formula. X--Ethanol content in the case sample, in milligrams per 100 milliliters (mg/100 mL); Y--peak area ratio of ethanol and internal standard tert-butanol in the case sample; a--intercept of linear equation; b--the slope of the linear equation. 7.2.3.3 Calculate Relative Phase Difference The relative difference between the ethanol content of the two case samples in parallel operation is calculated according to formula (2). RD (%) = 0 XX 21  (2) In the formula. RD-relative phase difference; X1, X2--the quantitative determination of ethanol content in two cases; X-Mean value of ethanol content of two case samples measured in parallel.

8 Results Evaluation

8.1 Evaluation of Qualitative Results 8.1.1 Positive results It is normal that no chromatographic peak of ethanol appears in the blank sample, and that of both ethanol and t-butanol in the added sample is normal. blank And the addition sample is normal, the relative retention time (or retention time) of the chromatographic peak in the case sample and the relative retention time of the ethanol in the addition sample (Or retention time) comparison, the relative error is within ± 2%. After testing with different chromatographic conditions, if the results are consistent, it is determined that the Contains ethanol. 8.1.2 Negative results The blank and added samples are normal, the t-butanol peak of the case sample is normal, and when there is no peak of ethanol, the test result is considered negative. Sex. If the result of the blank and/or added sample is abnormal, it indicates that the operation is wrong and the result is invalid. It should be re-examined. 8.1.3 Detection limit The detection limit of ethanol in blood by this method was 1 mg/100 mL. 8.2 Evaluation of quantitative results 8.2.1 Calibration curve The linear correlation coefficient r of the ethanol content calibration curve is not less than 0.999, and the calibration curve is valid. 8.2.2 Quantitative results When the relative difference between the measurement results of two case samples measured in parallel is not more than 10% (the blood sample with clot should not exceed 15%), The quantitative results are valid, and the quantitative results are calculated based on the average of the measurement results of the two case samples, otherwise the measurement needs to be repeated. 8.2.3 Limit of quantitation The limit of quantification of ethanol in blood by this method was 5 mg/100 mL. The qualitative analysis results of the case samples were positive, and the quantitative analysis of ethanol content When the amount is less than 5 mg/100 mL, the test result is that ethanol is detected in the blood, and the content is less than 5 mg/100 mL.

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GA/T 842-2019_English be delivered?

Answer: Upon your order, we will start to translate GA/T 842-2019_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GA/T 842-2019_English with my colleagues?

Answer: Yes. The purchased PDF of GA/T 842-2019_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GA/T 842-2019?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GA/T 842-2019 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.