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Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
GA/T 842-2019 | English | 229 |
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Examination methods for ethanol concentration in blood
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GA/T 842-2019
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GA/T 842-2009 | English | 439 |
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Analysis method for ethanol concentration in blood
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GA/T 842-2009
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PDF similar to GA/T 842-2019
Basic data Standard ID | GA/T 842-2019 (GA/T842-2019) | Description (Translated English) | Examination methods for ethanol concentration in blood | Sector / Industry | Public Security (Police) Industry Standard (Recommended) | Classification of Chinese Standard | R86 | Classification of International Standard | 11.040.20 | Word Count Estimation | 10,112 | Date of Issue | 2019 | Date of Implementation | 2019-05-01 | Issuing agency(ies) | Ministry of Public Security |
GA/T 842-2019: Examination methods for ethanol concentration in blood---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Examination methods for ethanol concentration in blood
ICS 11.040.20
R 86
People's Republic of China Public Safety Industry Standard
GA
Replaces GA/T 842-2009
Test method for blood alcohol content
Published by the Ministry of Public Security of the People's Republic of China
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces GA/T 842-2009 "Test method for blood alcohol content". Compared with GA/T 842-2009,
The main technical changes are as follows.
-Added "normative references" (see Chapter 2);
-Revised the requirements for "reagents" (see Chapter 5, Chapter 5 of the.2009 edition);
-Deleted the content of "n-propanol" (see Chapter 5 of the.2009 edition);
-Added requirements for "analytical balances" (see Chapter 6);
-Deleted “PTFE film” (see Chapter 6 of the.2009 edition);
-Revised the requirements for "sample preparation" (see 7.1.1, 7.1.1 of the.2009 edition);
-The requirements of “Reference Conditions for Headspace Sample Analysis” have been modified (see 7.1.2.2, 7.1.2.2 of the.2009 edition);
-Deleted the content of "headspace manual injection" (see 7.1.2.3.2 of the.2009 version);
-Revised the requirements for "sample preparation" (see 7.2.1, 7.2.1 of.2009 edition);
-The requirements for "quantification" have been modified (see 7.2.3.2, 7.2.3.2 of the.2009 edition);
-Revised the requirements for "quantitative results" (see 8.2.2,.2009 version 8.2.2);
-The name of “Determination Limit” has been modified (see 8.2.3, 8.2.3 of the.2009 edition);
-Removed Appendix A (see Appendix A of the.2009 edition);
-Removed Appendix B (see Appendix B of the.2009 edition).
This standard was proposed by Tianjin Public Security Traffic Management Bureau.
This standard is under the jurisdiction of the National Road Traffic Management Standardization Technical Committee.
This standard is drafted by. Tianjin Public Security Traffic Management Bureau.
This standard participated in the drafting unit. Shanxi Provincial Public Security Department Traffic Police Corps.
The main drafters of this standard. Shen Yuming, Liu Junfang, Cai Hongyan, Zhuang Xuying, Xing Jianyu, Li Dezhuang, Liu Zhao, Yao Chenyu, Li Huaiyu,
Yang Kun.
The previous versions of the standards replaced by this standard are.
--GA/T 842-2009.
Test method for blood alcohol content
1 Scope
This standard specifies the headspace gas chromatography test method for blood alcohol content.
This standard applies to the qualitative and quantitative analysis of alcohol in the blood of persons during road traffic law enforcement activities.
2 Normative references
The following documents are essential for the application of this document. For dated references, only the dated version applies to this document.
For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682-2008 Analytical laboratory water specifications and test methods
GB 19522 Threshold and test of blood and breath alcohol content of vehicle driver
GA/T 122 Toxicology Glossary
3 terms and definitions
The terms and definitions defined in GB 19522 and GA/T 122 apply to this document.
4 Principle
This method uses the volatile nature of alcohol (hereinafter referred to as ethanol), uses tert-butanol as an internal standard, and uses a headspace gas chromatography flame ionization detector.
Detection; comparison with ethanol standards in parallel, qualitative by retention time or relative retention time, internal standard method using ethanol
The peak area ratio was quantified.
5 reagents
Unless otherwise specified, the reagents used in this standard are chromatographically pure. The test water is secondary water (see GB/T 6682-2008).
a) ethanol;
b) tert-butanol;
c) Ethanol standard working solution. Precisely pipette or weigh an appropriate amount of absolute ethanol standard, and prepare 1000 mg/100 mL ethanol stock with water.
Liquid, sealed, refrigerated storage, the use period is 6 months. Dilute the ethanol stock solution by multiples with water to make a series of concentrations used in the test
Standard working solution of ethanol, the use period is 3 months;
d) tert-butanol internal standard working solution. accurately absorb or weigh the appropriate amount of tert-butanol standard, and prepare 4 mg/100 mL tert-butanol internal standard with water
The working solution is sealed and stored under refrigeration for a period of 3 months.
6 Equipment
The headspace gas chromatography test method should have the following equipment.
a) Gas chromatograph. equipped with hydrogen flame ionization detector (FID);
b) headspace autosampler;
c) Analytical balance. Sensitivity 0.1mg;
d) precision pipettes;
e) sample vials;
f) silicone rubber pads;
g) aluminum caps;
h) Sealing pliers.
7 Operation method
7.1 Qualitative analysis
7.1.1 Sample preparation
7.1.1.1 Case sample preparation
Take 0.10 mL of whole blood to be tested and 0.50 mL of tert-butanol internal standard working solution into a sample bottle, cover with a silicone rubber pad, and seal aluminum with sealing forceps.
Cap, mix well, to be tested.
7.1.1.2 Preparation of blank samples and added samples
Take 1 mg/100 mL ethanol standard working solution 0.10 mL and tert-butanol internal standard working solution 0.50 mL into the sample bottle as the detection limit
Add samples, and take another 0.10 mL of blank whole blood and 0.50 mL of tert-butanol internal standard working solution into the sample bottle as a blank sample. As per 7.1.1.1
Method operation, parallel control analysis with case samples.
7.1.2 Detection
7.1.2.1 Reference conditions for gas chromatography detection
The reference conditions for gas chromatography detection are shown in Table 1. It can be adjusted according to the actual situation of different brands of instruments and different samples.
Table 1 Reference conditions for gas chromatography detection
reference
condition
Column 1)
Column temperature
Carrier gas flow rate
mL/min
Detector
Detector temperature
Inlet temperature
DB-ALC1 (30 m × 0.32 mm × 1.8 μm) hair
Thin column or equivalent column
Flame off
Sub-test
Tester
(FID)
250.200
DB-ALC2 (30 m × 0.32 mm × 1.2μm) hair
Thin column or equivalent column
PLOT-Q (30 m × 0.53 mm × 20.00 μm) hair
Thin column or equivalent column
5 rbowax 20 M/Carbopack (80 ~ 120
Mesh) 2 m × 2 mm packed column or equivalent column
70 20 ~ 40
7.1.2.2 Reference Conditions for Headspace Sample Analysis Reference Conditions for Headspace Autosampler
a) Heating box temperature. 65 ℃;
1) DB-ALC1 and DB-ALC2 are suitable examples of commercially available products. This information is given for the convenience of the users of this standard and does not imply that
Approved.
b) Loop temperature. 105 ℃;
c) Transmission line temperature. 110 ℃;
d) Heating equilibration time of sample bottle. 10.00 min;
e) Sample bottle pressurization time. 0.10 min;
f) Filling time of the loop. 0.10 min;
g) Loop equilibrium time. 0.05 min;
h) Injection time. 1.00 min.
7.1.2.3 Injection
Place the prepared sample on the headspace autosampler sample rack, prepare an injection method, and run it.
7.1.3 Records and calculations
Record the retention times of t-butanol, ethanol standards, and suspicious ethanol peaks in each sample, and calculate the ethanol relative to t-butanol in each sample
Relative retention time value.
7.2 Quantitative analysis
7.2.1 Sample preparation
7.2.1.1 Case sample preparation
Take 0.10 mL of the whole blood to be tested and add 0.50 mL of tert-butanol internal standard working solution, the sample preparation is the same as 7.1.1.1.
7.2.1.2 Preparation of calibration curve
Configure a series of standard ethanol working solutions. Take a series of 0.10 mL of each standard working solution and t-butanol internal standard working solution
0.50 mL was added to each sample vial. The sample preparation was the same as 7.1.1.1.
The concentration of the ethanol standard working solution series used to prepare the calibration curve should be no less than six step concentrations, and the ethanol concentration of the sample to be measured should be in the school
Within the linear range of the quasi-curve.
7.2.2 Detection and recording
According to 7.1.2, record the peak area values of ethanol and t-butanol in the case samples and the series of standard working solution samples.
7.2.3 Calculation
7.2.3.1 Calibration curve
Linear regression of the mass concentration of ethanol using the ratio of the peak area of ethanol to t-butanol in a series of standard working solution samples yields the line
Sexual equation.
7.2.3.2 Quantitative
According to the peak area values of ethanol and internal standard tert-butanol in the case sample, calculate the ethanol content X in the case sample according to formula (1).
X =
aY
(1)
In the formula.
X--Ethanol content in the case sample, in milligrams per 100 milliliters (mg/100 mL);
Y--peak area ratio of ethanol and internal standard tert-butanol in the case sample;
a--intercept of linear equation;
b--the slope of the linear equation.
7.2.3.3 Calculate Relative Phase Difference
The relative difference between the ethanol content of the two case samples in parallel operation is calculated according to formula (2).
RD (%) = 0
XX 21
(2)
In the formula.
RD-relative phase difference;
X1, X2--the quantitative determination of ethanol content in two cases;
X-Mean value of ethanol content of two case samples measured in parallel.
8 Results Evaluation
8.1 Evaluation of Qualitative Results
8.1.1 Positive results
It is normal that no chromatographic peak of ethanol appears in the blank sample, and that of both ethanol and t-butanol in the added sample is normal. blank
And the addition sample is normal, the relative retention time (or retention time) of the chromatographic peak in the case sample and the relative retention time of the ethanol in the addition sample
(Or retention time) comparison, the relative error is within ± 2%. After testing with different chromatographic conditions, if the results are consistent, it is determined that the
Contains ethanol.
8.1.2 Negative results
The blank and added samples are normal, the t-butanol peak of the case sample is normal, and when there is no peak of ethanol, the test result is considered negative.
Sex. If the result of the blank and/or added sample is abnormal, it indicates that the operation is wrong and the result is invalid. It should be re-examined.
8.1.3 Detection limit
The detection limit of ethanol in blood by this method was 1 mg/100 mL.
8.2 Evaluation of quantitative results
8.2.1 Calibration curve
The linear correlation coefficient r of the ethanol content calibration curve is not less than 0.999, and the calibration curve is valid.
8.2.2 Quantitative results
When the relative difference between the measurement results of two case samples measured in parallel is not more than 10% (the blood sample with clot should not exceed 15%),
The quantitative results are valid, and the quantitative results are calculated based on the average of the measurement results of the two case samples, otherwise the measurement needs to be repeated.
8.2.3 Limit of quantitation
The limit of quantification of ethanol in blood by this method was 5 mg/100 mL. The qualitative analysis results of the case samples were positive, and the quantitative analysis of ethanol content
When the amount is less than 5 mg/100 mL, the test result is that ethanol is detected in the blood, and the content is less than 5 mg/100 mL.
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