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SN/T 5334.7-2020 English PDF

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SN/T 5334.7-2020: (Digital PCR detection method for genetically modified plant products - Part 7: Genetically modified alfalfa)
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 5334.7-2020	179	Add to Cart	3 days	(Digital PCR detection method for genetically modified plant products - Part 7: Genetically modified alfalfa)	Valid

Similar standards

NY/T 2288 SN/T 3075 JB 2293 SN/T 5334.2 SN/T 5334.3 SN/T 5334.1

Basic data

Standard ID: SN/T 5334.7-2020 (SN/T5334.7-2020)
Description (Translated English): (Digital PCR detection method for genetically modified plant products - Part 7: Genetically modified alfalfa)
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 8,822
Date of Issue: 2020-12-30
Date of Implementation: 2021-07-01
Regulation (derived from): General Administration of Customs Announcement No. 136 [2020]
Issuing agency(ies): General Administration of Customs

SN/T 5334.7-2020: (Digital PCR detection method for genetically modified plant products - Part 7: Genetically modified alfalfa)

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of digital PCR for quantitatively detecting genetically modified plants and their derived products- Part 7.Genetically modified alfalfa The People's Republic of China Entry-Exit Inspection and Quarantine Industry Standards 2020-12-30 release 2021-07-01 implementation Issued by the General Administration of Customs of the People's Republic of China

Foreword

SN/T 5334-2020 "Digital PCR Detection Method for Genetically Modified Plant Products" is divided into 8 parts. --Part 1.General requirements and definitions; --Part 2.Genetically modified soybeans; --Part 3.Genetically modified corn; --Part 4.Genetically modified rape; --Part 5.Genetically modified cotton; --Part 6.Genetically Modified Potatoes; --Part 7.Genetically modified alfalfa; --Part 8.Genetically modified sugar beet. This part is part 7 of SN/T 5334-2020. This document was drafted in accordance with the rules given in GB/T 1.1-2020. Certain contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents. This document was proposed and managed by the General Administration of Customs of the People's Republic of China. This document was drafted by. Chinese Academy of Inspection and Quarantine Sciences, Guangzhou Customs Inspection and Quarantine Technology Center of the People’s Republic of China. The main drafters of this document. Liu Jin, Fu Wei, Gao Dongwei, Liu Erlong, Zhu Shuifang, Dong Jie, Guan Lijun, Xie Li. Digital PCR detection method for genetically modified plant products Part 7.Genetically Modified Alfalfa

1 Scope

This document specifies the quantitative detection method of genetically modified strains specific to imported and exported alfalfa by digital PCR (dPCR). This document is suitable for the quantitative detection of specific components of genetically modified alfalfa J101, J163, and KK179 strains in alfalfa seeds and alfalfa grass. The lower limit of quantitative detection in this document is 7.0 copies/μL, and the lower limit of qualitative detection is 1.4 copies/μL.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated references, the latest version (including all amendments) is applicable Used in this document. SN/T 5334.1 Digital PCR Method for Quantitative Detection of Genetically Modified Products Part 1.General Requirements and Definitions

3 Terms and definitions

The terms and definitions defined in SN/T 5334.1 apply to this document.

4 Principles of the method

See SN/T 5334.1.

5 Equipment and reagents

5.1 Endogenous gene and exogenous gene. the internal standard gene is acetyl-coenzyme A carboxylase gene (ACC); the exogenous sequence is strain J101, J163 and KK179 foreign gene insertion site 5'end cross-border sequence. Primers for dPCR quantitative detection of genetically modified strains in alfalfa See Appendix A for the probe sequence. 5.2 For other equipment and reagent requirements, please refer to SN/T 5334.1.

6 Method operation steps

6.1 General operation steps For general operation steps, see SN/T 5334.1. 6.2 Digital PCR reaction system Prepare the final reaction system of digital PCR according to Table 1. a. It is recommended to use the digital PCR platform currently on the market for experiments; b. The types of reagents added in the final reaction system and the total system volume may vary according to different digital PCR platforms. Perform different digital PCR When configuring the system of the platform, it is necessary to refer to the instructions of the digital PCR instrument of different platforms, and it is necessary to ensure that the final concentration of the components listed in Table 1 remains unchanged. 6.3 Digital PCR reaction program 6.3.1 Perform digital PCR amplification in accordance with the procedures in Table 2. a. According to the requirements of different digital PCR platforms, the hot start step in the reaction program can be modified, but the thermal cycling (amplification) step must not be modified. b. According to the requirements of different digital PCR platforms, it is possible to perform post-processing after the thermal cycling (amplification) step. The post-processing steps are different Digital PCR platform instructions were carried out. 6.3.2 For other requirements, see SN/T 5334.1.

7 Judgment and expression of results

See SN/T 5334.1 for calculation of results. - Both the internal standard gene and the foreign gene in the test result meet the negative and blank quality control requirements, indicating that no alfalfa has been detected The endogenous gene ACC is expressed as "alfalfa component not detected"; - In the test results, the internal standard gene result is positive, but the foreign gene meets the quality control requirements of negative and blank. Genetically modified strains"; - In the test results, both the internal standard gene and the exogenous genome meet the quality control requirements of the digital PCR test of the sample, indicating that 8 Anti-pollution measures The anti-pollution measures are specified in SN/T 5334.1.

9 Sample storage

For sample storage, see SN/T 5334.1.

Appendix A

(Informative appendix) Note. The underlined part is the alfalfa genome sequence, the shaded part is the upstream and downstream primer sequence, and the boxed part is the probe sequence A.4 Primer and probe sequence See Table A.1. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 5334.7-2020_English be delivered?

Answer: Upon your order, we will start to translate SN/T 5334.7-2020_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 5334.7-2020_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 5334.7-2020_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.