SN/T 5334.3-2020 English PDF

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SN/T 5334.3-2020: (Digital PCR detection method for genetically modified plant products - Part 3: Genetically modified corn)
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Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 5334.3-2020	159	Add to Cart	3 days	(Digital PCR detection method for genetically modified plant products - Part 3: Genetically modified corn)	Valid

Similar standards

NY/T 2288   SN/T 3075   JB 2293   SN/T 5334.2   SN/T 5334.4   SN/T 5334.1   

Basic data

Standard ID: SN/T 5334.3-2020 (SN/T5334.3-2020)
Description (Translated English): (Digital PCR detection method for genetically modified plant products - Part 3: Genetically modified corn)
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 7,785
Date of Issue: 2020-12-30
Date of Implementation: 2021-07-01
Regulation (derived from): General Administration of Customs Announcement No. 136 [2020]
Issuing agency(ies): General Administration of Customs

SN/T 5334.3-2020: (Digital PCR detection method for genetically modified plant products - Part 3: Genetically modified corn)

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of digital PCR for quantitatively detecting genetically modified plants and their derived products- Part 3.Genetically modified maize The People's Republic of China Entry-Exit Inspection and Quarantine Industry Standards 2020-12-30 release 2021-07-01 implementation Issued by the General Administration of Customs of the People's Republic of China

Foreword

SN/T 5334-2020 "Digital PCR Detection Method for Genetically Modified Plant Products" is divided into 8 parts. --Part 1.General requirements and definitions; --Part 2.Genetically modified soybeans; --Part 3.Genetically modified corn; --Part 4.Genetically modified rape; --Part 5.Genetically modified cotton; --Part 6.Genetically modified potatoes; --Part 7.Genetically modified alfalfa; --Part 8.Genetically modified sugar beet. This document is part 3 of SN/T 5334-2020. This document was drafted in accordance with the rules given in GB/T 1.1-2020. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents. This document was proposed and managed by the General Administration of Customs of the People's Republic of China. This document was drafted by. Chinese Academy of Inspection and Quarantine Sciences, Shenzhen Customs Food Inspection and Quarantine Technology Center of the People’s Republic of China. The main drafters of this document. Ling Xingyuan, Pan Guang, Zhu Pengyu, Fu Wei, Xiang Caiyu, Zhang Guiming, Zhu Shuifang, Huang Wensheng. Digital PCR detection method for genetically modified plant products Part 3.Genetically Modified Corn

1 Scope

This document specifies the digital PCR quantitative detection method for genetically modified strains in maize. This document is applicable to the quantitative detection of 10 genetically modified strains in corn seeds and leaves. See Appendix A for the detection limits of this document.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated references, the latest version (including all amendments) is applicable Used in this document. SN/T 5334.1 Digital PCR method for quantitative detection of genetically modified products Part 1.General requirements and definitions

3 Terms and definitions

The terms and definitions defined in SN/T 5334.1 apply to this document.

4 Principles of the method

See SN/T 5334.1.

5 Main equipment and reagents

5.1 Endogenous gene and exogenous gene. the endogenous gene is maize high mobility group proteins (HMG. high mobility group proteins); The foreign genes are lines 98140, DAS40278-9, VCO-01981-5 and MON87427 insertion site 5'end cross-border sequence, and Strains BVLA430101, Bt799, IE034, 12-5, C0010.3.7 and C0030.3.5 insert the 3'-end cross-border sequence at the insertion site. Primers and See Appendix A for the probe sequence. 5.2 For other equipment and reagent requirements, please refer to SN/T 5334.1.

6 Method operation steps

6.1 General operation steps For general operation steps, please refer to SN/T 5334.1. 6.2 Digital PCR reaction system See Table 1 for the preparation of real-time fluorescent PCR reaction system. Note. It is recommended to use the digital PCR platform on the market for experiments 6.3 Digital PCR reaction program See SN/T 5334.1 for other requirements, and see Table 2 for the digital PCR reaction program. a. According to the requirements of different digital PCR platforms, the hot start step in the reaction program can be modified, but the thermal cycle (amplification) must not be modified step. b. According to the requirements of different digital PCR platforms, it is possible to perform post-processing after the thermal cycling (amplification) step, which can be performed according to the requirements of different platforms. Seek to proceed.

7 Judgment and expression of results

See SN/T 5334.1 for calculation of results. The results are expressed as follows. - Both the internal standard gene and the foreign gene in the test result meet the negative and blank quality control requirements, indicating that no corn has been detected The endogenous gene Adh1, expressed as "no corn component detected"; - In the test results, the internal standard gene result is positive, but the foreign gene meets the quality control requirements of negative and blank, said Genetic strain"; - In the test results, both the internal standard gene and the exogenous genome meet the quality control requirements of the digital PCR test of the sample, indicating that 8 Anti-pollution measures The anti-pollution measures are specified in SN/T 5334.1.

9 Sample storage

For sample storage, see SN/T 5334.1.

Appendix A

(Informative appendix) Primer probe sequence, reaction program and detection limit ......

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