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SN/T 5334.4-2020 English PDF

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US$179.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 5334.4-2020: (Digital PCR Detection Method for Genetically Modified Plant Products - Part 4: Genetically Modified Rape)
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 5334.4-2020	179	Add to Cart	3 days	(Digital PCR Detection Method for Genetically Modified Plant Products - Part 4: Genetically Modified Rape)	Valid

Similar standards

NY/T 2288 SN/T 3075 JB 2293 SN/T 5334.2 SN/T 5334.3 SN/T 5334.1

Basic data

Standard ID: SN/T 5334.4-2020 (SN/T5334.4-2020)
Description (Translated English): (Digital PCR Detection Method for Genetically Modified Plant Products - Part 4: Genetically Modified Rape)
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 8,829
Date of Issue: 2020-12-30
Date of Implementation: 2021-07-01
Regulation (derived from): General Administration of Customs Announcement No. 136 [2020]
Issuing agency(ies): General Administration of Customs

SN/T 5334.4-2020: (Digital PCR Detection Method for Genetically Modified Plant Products - Part 4: Genetically Modified Rape)

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of digital PCR for quantitatively detecting genetically modified plants and their derived products- Part 4.Genetically modified canola The People's Republic of China Entry-Exit Inspection and Quarantine Industry Standards 2020-12-30 release 2021-07-01 implementation Issued by the General Administration of Customs of the People's Republic of China

Foreword

SN/T 5334-2020 "Digital PCR Detection Method for Genetically Modified Plant Products" is divided into 8 parts. --Part 1.General requirements and definitions; --Part 2.Genetically modified soybeans; --Part 3.Genetically modified corn; --Part 4.Genetically modified rape; --Part 5.Genetically modified cotton; --Part 6.Genetically Modified Potatoes; --Part 7.Genetically modified alfalfa; --Part 8.Genetically modified sugar beet. This document is part 4 of SN/T 5334-2020. This document was drafted in accordance with the rules given in GB/T 1.1-2020. Certain contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents. This document was proposed and managed by the General Administration of Customs of the People's Republic of China. Drafting organizations of this document. Chinese Academy of Inspection and Quarantine Sciences, Shenzhen Customs Food Inspection and Quarantine Technology Center of the People’s Republic of China, Shanghai Customs of the People's Republic of China, Gongbei Customs Inspection and Quarantine Technology Center of the People's Republic of China. The main drafters of this document. Pan Guang, Li Xiang, Yao Lifeng, Cai Jiaoying, Ling Xingyuan, Wang Xiaoyu, You Shuzhu. Digital PCR detection method for genetically modified plant products Part 4.Genetically Modified Canola

1 Scope

This document specifies the digital PCR quantitative detection method for genetically modified strains in rapeseed. This document is applicable to the quantitative detection of 11 genetically modified lines in rapeseeds and leaves. See Appendix A for the detection limits of this document.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated references, the latest version (including all amendments) is applicable Used in this document. SN/T 5334.1 Digital PCR Method for Quantitative Detection of Genetically Modified Products Part 1.General Requirements and Definitions

3 Terms and definitions

The terms and definitions defined in SN/T 5334.1 apply to this document.

4 Principle

See SN/T 5334.1.

5 Equipment and reagents

5.1 Endogenous gene and exogenous gene. the internal standard gene is the rape phosphoenolpyruvate carboxylase gene (PEP); the exogenous sequence is the product Lines 73496, T45, MON88302 and OXY235 foreign gene insertion sites 5'end cross-border sequence, and lines Topas19/2, MS1, The foreign sequences of MS8, Rf1, Rf2, Rf3 and RT73 are inserted into the cross-border sequence at the 3'end of the site. See Appendix A for primer and probe sequences. 5.2 For other equipment and reagent requirements, please refer to SN/T 5334.1.

6 Method operation steps

6.1 General operation steps For general operation steps, see SN/T 5334.1. 6.2 Digital PCR reaction system 6.2.1 Two-channel digital PCR reaction system See Table 1 for the preparation of the dual-channel digital PCR reaction system. Note. It is recommended to use the digital PCR platform currently on the market for experiments. 6.2.2 Single-channel digital PCR reaction system The single-channel digital PCR reaction system preparation is shown in Table 2. a. It is recommended to use the digital PCR platform currently on the market for experiments. 6.3 Digital PCR reaction program The digital PCR reaction program is shown in Table 3. a. According to the requirements of different digital PCR platforms, the hot start step in the reaction program can be modified, but the thermal cycling (amplification) step must not be modified. b. According to the requirements of different digital PCR platforms, post-processing may be carried out after the thermal cycling (amplification) step, which can be carried out according to the requirements of different platforms.

7 Judgment and expression of results

See SN/T 5334.1 for calculation of results. The results are expressed as follows. - In the test results, both the internal standard gene and the foreign gene meet the negative and blank quality control requirements, indicating that no rape has been detected. Source gene PEP, expressed as "No oilseed rape component detected"; - In the test results, the internal standard gene result is positive, but the foreign gene meets the quality control requirements of negative and blank, said Genetically modified strains"; - In the test results, both the internal standard gene and the exogenous genome meet the quality control requirements of the digital PCR test of the sample, indicating that 8 Anti-pollution measures The anti-pollution measures are specified in SN/T 5334.1.

9 Sample storage

For sample storage, see SN/T 5334.1.

Appendix A

(Informative appendix) Primer probe sequence, reaction program and detection limit ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 5334.4-2020_English be delivered?

Answer: Upon your order, we will start to translate SN/T 5334.4-2020_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 5334.4-2020_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 5334.4-2020_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.