SN/T 4824-2017 English PDF

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SN/T 4824-2017: Detection method for Akabane virus of bovine and ovine by LAMP
Status: Valid

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SN/T 4824-2017	209	Add to Cart	3 days	Detection method for Akabane virus of bovine and ovine by LAMP	Valid

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Basic data

Standard ID: SN/T 4824-2017 (SN/T4824-2017)
Description (Translated English): Detection method for Akabane virus of bovine and ovine by LAMP
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Word Count Estimation: 9,952
Date of Issue: 2017-07-21
Date of Implementation: 2018-03-01
Regulation (derived from): State-Quality-Inspection-Accreditation (2017) 337
Issuing agency(ies): General Administration of Customs

SN/T 4824-2017: Detection method for Akabane virus of bovine and ovine by LAMP

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Identification method of ring - mediated isothermal amplification of) People's Republic of China entry and exit inspection and quarantine industry standards Bovine sheep red feather disease virus loop-mediated isothermal amplification Detection method Released on.2017-07-21 2018-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, South China Agricultural University. The main drafters of this standard. Yu Haiqiong, Cai Xianquan, Cheng Yiyi, Jia Kun, Zhao Wei, Wang Ying, Tian Chunjian, Chen Fang, Chen Ru, Lin Zhixiong. Bovine sheep red feather disease virus loop-mediated isothermal amplification Detection method

1 Scope

This standard specifies the ring-mediated isothermal amplification detection method for bovine and sheep red feather disease virus. This standard is applicable to the rapid initial screening test of cattle and sheep red feather virus nucleic acid.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 18088 entry and exit animal quarantine sampling

3 Abbreviations

The following abbreviations apply to this document. AKAV Akabanevirus LAMP loop-mediated isothermal amplification (Loop-mediated isothermalamplification)

4 Overview of Akabane disease

The Akabies virus is a single-stranded RNA virus belonging to the genus Bunnivirus of the Bunny virus. See Appendix A for a summary of the red feather disease caused by it.

5 reagent

5.1 RNA extraction kit. 5.2 BstDNA polymerase. 5.3 Betaine Betaine. 5.4 0.01 mol/L PBS buffer (see Appendix B). 5.5 10 x Thermopol buffer. 5.6 100 mmol/L MgSO4. 5.7 10 mmol/LdNTP. 5.8 5×AMVBuffer and AMVRTase. 5.9 AKAV cell culture (inactivated). 5.10 Internal Primer (FIP BIP). 5.11 External primer (F3 B3). 5.12 RNasin. ......

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