SN/T 4733-2016 English PDF

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SN/T 4733-2016: Detection and identification of Alternaria triticina Prasada and Prabhu
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4733-2016	229	Add to Cart	3 days	Detection and identification of Alternaria triticina Prasada and Prabhu	Valid

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Basic data

Standard ID: SN/T 4733-2016 (SN/T4733-2016)
Description (Translated English): Detection and identification of Alternaria triticina Prasada and Prabhu
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 10,166
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 4733-2016: Detection and identification of Alternaria triticina Prasada and Prabhu

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Alternaria triticina Prasada and Prabhu China's entry-exit inspection and quarantine industry standards Quarantine and identification methods for wheat leaf blight Published on.2016-12-12 2017-07-01 Implementation China The State Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission. This standard was drafted by the Xinjiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China and the Agricultural Technology Promotion Station of the Xinjiang Production and Construction Corps. Drafters of this standard. Zhang Xianglin, Zhao Bingmei, Wang Hao, Zhang Wei, Zhang Xiaoju, and Zhang Yu. Quarantine and identification methods for wheat leaf blight

1 Scope

This standard specifies the wheat leaf blight [Alternariatriticina Prasada This standard is applicable to the quarantine and identification of wheat leaf blight bacteria in imported wheat seeds, raw grains and field diseased plants.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. SN/T 1538.1 Guidelines for Preparation of Culture Media Part 1. General Principles for Quality Assurance for Preparation of Laboratory Culture Media SN/T 1809 Entry and Exit Plant Seed Quarantine Procedures

3 basic information of wheat leaf blight

Scientific name. Alternariatritwina Prasada English name.leafblightofwheat Taxonomic status. Ascomycota; Dothideomycetes; Pleosporales; Corynespora Pleosporaceae; Alternaria. See Appendix A and Appendix B for other relevant information on wheat leaf blight.

4 Methodology

According to the morphological characteristics of pathogenic bacteria, culture traits, hazard symptoms on the host, and PCR-specific amplified fragment characteristics as wheat leaves The identification basis of the bacteria.

5 Instruments and supplies

5.1 Instruments Biological Microscope, Clean Bench, PCR Amplification Device, Electrophoresis Apparatus, Gel Imager, High Speed Refrigerated Centrifuge, Light Incubator, Electronic Day Flat (1/1000g), constant temperature water bath, autoclave, refrigerator. 5.2 Appliances Hand-held magnifying glass, petri dish, alcohol lamp, tweezers, scissors, scalpel, sample sieve [φ1.7mm (10 mesh)], pruning shears, inoculation needle, glass carrier Tablets, coverslips, sealing membranes, bacteria preservation tubes, centrifuge tubes (1.5 mL), adjustable micropipettes (2.5 μL to.200 μL).

6 Reagents and culture media

6.1 Reagents Glucose, agar powder, sodium hypochlorite, hydrochloric acid, magnesium chloride, SDS, EDTA, Tris, CTAB, EDTA, anhydrous ethanol, trichloromethane, ......

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