SN/T 4732-2016 English PDF

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SN/T 4732-2016: Detection and identification of Xanthomonas campestris pv. musacearum
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Basic data

Standard ID: SN/T 4732-2016 (SN/T4732-2016)
Description (Translated English): Detection and identification of Xanthomonas campestris pv. musacearum
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 18,179
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 4732-2016: Detection and identification of Xanthomonas campestris pv. musacearum

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Xanthomonas campestris pv.musacearum China's entry-exit inspection and quarantine industry standards Quarantine and identification methods for banana bacterial wilt pathogens Published on.2016-12-12 2017-07-01 Implementation China The State Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission. This standard was drafted by. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China and Shenzhen Institute of Inspection and Quarantine. The main drafters of this standard. Feng Jianjun, Wang Ying, Zhang Guiming, Long Hai, Wu Shaojing, Wang Hongying. Quarantine and identification methods for banana bacterial wilt pathogens

1 Scope

The current standard specifies the quarantine and identification methods for Xanthomonas campestris pv. Musacearum. This standard applies to the quarantine and identification of banana bacterial wilt pathogens in banana stems, leaves, fruits and other tissue materials.

2 Basic Information on Banana Bacterial Wilt Bacteria

Scientific name. Xanthomonascampestrispv.musacearum (Yirgou Synonyms. Xanthomonasvasicolapv.musacearum Arituaetal2008 Disease English name. Bananaxanthomonaswilt Taxonomic status. Procerotae, Proteobacteria, Gammaproteobacteria, From the order of the Xanthomonadales, the Xanthomonadaceae, and the Xanthomonas. The main ways of long-distance transmission are the transmission of infected fruits, leaves and other plant materials and insects. See Appendix A for additional information.

3 Methodology

This standard is mainly based on the symptoms of banana bacterial wilt pathogens, colony morphological characteristics, physiological and biochemical characteristics, pathogenicity and GspD gene characteristics and small molecule polypeptide target molecular marker characteristics and other relevant information and formulate. 4 Instruments, Apparatus and Reagents 4.1 Instruments PCR apparatus, ultra-clean bench, autoclave, ice maker, desktop mini centrifuge, ultra-low temperature refrigerator, conventional refrigerator, vortex oscillator, Electrophoresis apparatus, gel imaging system, matrix-assisted laser desorption ionization time of flight mass spectrometer (MALDI-TOFMS). 4.2 Appliances Adjustable pipettes (up to 20 μL,.200 μL, 1000 μL) and related tips, centrifuge tubes (2 mL), PCR tubes, graduated cylinders, and burners Cups, alcohol lamps, tweezers, etc. 4.3 Reagents and Culture Media Unless otherwise specified, all reagents are analytically pure or biochemical reagents. See Appendix B, Appendix C, Appendix D, and Appendix E for media and reagent formulations.

5 Quarantine identification

5.1 On-site quarantine 5.1.1 Observation of symptoms Carefully examine the on-site observation of banana leaf, stem and fruit tissue and other symptoms. See A.4 for symptom description. ......

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