SN/T 4729-2016 English PDF

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SN/T 4729-2016: Detection and identification of Seifertia azaleae (Peck)Partridge and Morgan-Jones
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Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4729-2016	329	Add to Cart	3 days	Detection and identification of Seifertia azaleae (Peck)Partridge and Morgan-Jones	Valid

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Basic data

Standard ID: SN/T 4729-2016 (SN/T4729-2016)
Description (Translated English): Detection and identification of Seifertia azaleae (Peck)Partridge and Morgan-Jones
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 14,111
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 4729-2016: Detection and identification of Seifertia azaleae (Peck)Partridge and Morgan-Jones

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Seifertia azaleae (Peck)Partridge and Morgan-Jones China's entry-exit inspection and quarantine industry standards The quarantine and identification method of aphid bud disease Published on.2016-12-12 2017-07-01 Implementation China The State Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission. This standard was drafted by. China Academy of Inspection and Quarantine. Drafters of this standard. Wu Pinshan, Du Hongzhong, Lei Rong, Shao Si, Yan Jin, and Zhang Qiuji. The quarantine and identification method of aphid bud disease

1 Scope

This standard specifies the quarantine and identification methods for aphid blight germs. This standard applies to aphid blight fungi carried in flower buds, leaf buds and shoots of Rhododendron L. plants. Quarantine identification.

2 basic information of germs

Scientific name. Seifertiaazaleae (Peck) Partridge and Morgan-Jones English name. Budblight, Budandtwigblight Taxonomic status. Fungi, Ascomycota, Asperycota, Erythomycetes, Chaetothyri- Ales, Herpetrichielaceae. Route of transmission. The germs mainly remain on the disease buds or overwinter. Infested by the base of bud scales in the leap year and entered the pith, impeding water in the shoots Convey the buds and shoots wither. Proximity is mainly caused by vector insects such as leafhoppers, bees, etc., and splashes of rain droplets. Long-distance transmission is mainly through the band Diseased plants spread. See Appendix A for additional information on aphid blight pathogens.

3 Methodology

The morphological characteristics and ITS gene sequence alignment characteristics of A. vegetative aphid were used as identification criteria.

4 Instruments and Equipment

4.1 Equipment and equipment Biological microscope (with measurement function), stereo microscope, clean bench, biological incubator, balance, autoclave, desktop high-speed centrifugation Machine, pH meter, water bath, PCR, electrophoresis, gel imager. 4.2 Appliances Beakers, Erlenmeyer flasks, graduated cylinders, test tubes, petri dishes, alcohol lamps, tweezers, scissors, scalpels, inoculation needles, slides, coverslips, plastic mortars, Pipettes, pipette tips, centrifuge tubes, liquid nitrogen tanks.

5 Reagents and culture media

5.1 Reagents Glucose, agar powder, sodium hypochlorite, streptomycin sulfate, liquid nitrogen, Tris-HCl, MgCl2, HCl, EDTA, NaOH, NaOAC, KCl, Tris, CTAB, TAE, Ethanol, Phenol, EB, Chloroform, Isopropanol (Isopropanol), Isoamylalcohol, Proteinase K, Taq DNA Polymerase, dNTPs, Primer ITS5/ITS4. ......

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