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Detection and identification of Colletotrichum orbiculare (Berk. Et Mont. ) Arx
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Basic data
| Standard ID | SN/T 4650-2016 (SN/T4650-2016) |
| Description (Translated English) | Detection and identification of Colletotrichum orbiculare (Berk. Et Mont. ) Arx |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B16 |
| Word Count Estimation | 14,116 |
| Date of Issue | 2016-08-23 |
| Date of Implementation | 2017-03-01 |
| Regulation (derived from) | State-Quality-Inspection-Accredidation (2016) No.438 |
| Issuing agency(ies) | General Administration of Customs |
SN/T 4650-2016: Detection and identification of Colletotrichum orbiculare (Berk. Et Mont. ) Arx
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Identification method of anthracnose pathogens)
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Method for quarantine and identification of anthracnose
Published on.2016-08-23
2017-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard is drafted by. Xinjiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Jiangxi Entry-Exit Inspection of the People's Republic of China
Epidemic Bureau, Shihezi University, Xinjiang Academy of Agricultural Sciences.
The main drafters of this standard. Wang Wei, Zhang Xiaoju, Huang Lili, Sun Yanfei, Zhang Xianglin, Zhang Wei, Li Yawei, Zhang Yu, Han Sheng, Yang Du.
Method for quarantine and identification of anthracnose
1 Scope
This standard specifies the quarantine and identification methods for Colletotrichumorbiculare (Berk.Et.Mont.) Arx.
This standard is applicable to the quarantine and identification of melon anthracnose in seeds, plants and fruits of import and export melon plants.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
SN/T 1809 entry and exit plant seed quarantine regulations
3 Basic information on anthracnose
Scientific name. Colletotrichumorbiculare (Berk.EtMont.)Arx
Synonym. Colletotrichumlagenarium (Pass.) El.etHalst.
Asexual generation scientific name. Colletotrichumorbiculare (Berk.EtMont.) Arx.
Sexual generation scientific name. Glomerellalagenaria (Pass.) WatanableetTamura
Classification status. Ascomycota, Pezizales, Pezizaceae, Periwinkle
Glomerella; alleles are Melanconiales, Melanconiaceae, Colleto-
Ttricum)
4 Principle of the method
Symptoms, culture traits, morphological characteristics, pathogenicity and PCR-specific amplification test results of anthracnose fungus
Basis for quarantine identification of bacteria.
5 instruments and main reagents
5.1 Instrumentation
Biological microscope, stereoscopic mirror, high-speed refrigerated centrifuge, pure water meter, ultra-clean workbench, autoclave, ice machine, PCR instrument, condensation
Glue imaging system, electrophoresis instrument, incubator, ultra-low temperature refrigerator, conventional refrigerator, vortex oscillator.
5.2 main reagent
All reagents are either analytical or biochemical reagents unless otherwise stated.
1% sodium hypochlorite, DNA extraction related reagents (box), PCR related reagents, electrophoresis reagents.
Potato dextrose agar medium (PDA) (see Appendix A for preparation methods).
...