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Quarantine protocol for swine hepatitis E
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Basic data
| Standard ID | SN/T 4235-2015 (SN/T4235-2015) |
| Description (Translated English) | Quarantine protocol for swine hepatitis E |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Word Count Estimation | 13,195 |
| Date of Issue | 2015-05-26 |
| Date of Implementation | 2016-01-01 |
| Regulation (derived from) | State Quality-Inspection-accreditation [2015] No.224 |
| Issuing agency(ies) | General Administration of Customs |
SN/T 4235-2015: Quarantine protocol for swine hepatitis E
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for swine hepatitis E
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Swine hepatitis hepatitis quarantine technical specification
Released on.2015-05-26
2016-01-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shanghai Entry-Exit Inspection and Quarantine
Bureau, Beijing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shanghai Animal Disease Prevention and Control Center.
The main drafters of this standard. Yu Haiqiong, Zhang Qiang, Qiao Caixia, Luo Changbao, Zhao Wei, Cai Xianquan, Liu Peihong, Zhou Jinping, Li Shuqing, Wu Xiaowei.
Swine hepatitis hepatitis quarantine technical specification
1 Scope
This standard specifies the clinical diagnosis of porcine hepatitis E, enzyme-linked immunosorbent assay, real-time fluorescent polymerase chain reaction detection technology.
This standard applies to the diagnosis and epidemiological investigation of swine hepatitis E.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 18088 entry and exit animal quarantine sampling
GB/T 19489 General requirements for laboratory biosafety
3 Abbreviations
The following abbreviations apply to this document.
HEV. Hepatitis E virus
BSA. Bovine Serum Albumin
HRP. horseradish peroxidase
OD value. optical density value
ELISA. enzyme-linked immunosorbent assay
Fluorescence RT-PCR. real-time fluorescent polymerase chain reaction
4 reagents
4.1 HEV positive control serum.
4.2 HEV negative control serum.
4.3 HEV antigen.
4.4 Bovine serum albumin.
4.5 HRP-labeled rabbit anti-porcine IgG.
4.6 0.01 mol/L PBS buffer.
4.7 Antigen dilution (see Appendix A).
4.8 Washing liquid (see Appendix A).
4.9 Tween-20.
4.10 Blocking solution (see Appendix A).
4.11 Reagent (see Appendix A).
4.12 Stop solution (see Appendix A).
4.13 TRIZOL.
4.14 chloroform.
...
