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Detection and identification of Squash mosaic virus
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Basic data
| Standard ID | SN/T 4178-2015 (SN/T4178-2015) |
| Description (Translated English) | Detection and identification of Squash mosaic virus |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B16 |
| Classification of International Standard | 07.080 |
| Word Count Estimation | 17,174 |
| Date of Issue | 2015-02-09 |
| Date of Implementation | 2015-09-01 |
| Quoted Standard | SN/T 1809; SN/T 2122 |
| Regulation (derived from) | State-Quality-Inspection-Accreditation [2015] 59 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the method for the detection and identification of squash mosaic virus serology, molecular biology. This standard applies to the detection and identification of plant squash mosaic virus. |
SN/T 4178-2015: Detection and identification of Squash mosaic virus
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Quarantine and identification of squash mosaic virus)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Squash mosaic virus quarantine and identification methods
Issued on. 2015-02-09
2015-09-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Structure does not bear the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine of Hunan People's Republic of China
Bureau, People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau, Wuhan Technology Development Co., Ltd. wah thiophene satisfied.
The main drafters of this standard. Liao Furong, Huangying Bo, Lin Shiming, Liang Miao, Wu Yuan, Chen Qing, Deng Cong Liang, Zhao Xiaoli, Nie edge, Chen Yun.
Squash mosaic virus quarantine and identification methods
1 Scope
This standard specifies the method for the detection and identification of squash mosaic virus serology, molecular biology.
This standard applies to the detection and identification of plant squash mosaic virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 1809 export plant quarantine rules for seed
SN/T 2122 entry and exit quarantine of plants and plant products sampled
3 squash mosaic virus Basic Information
Chinese name. squash mosaic virus
English name. Squashmosaicvirus
Abbreviation. SqMV
Taxonomic position. small RNA virus mesh (Picornavirales), cowpea associated virus family (Secoviridae), cowpea mosaic virus subfamily
(Comovirinae), cowpea mosaic virus genus (Comovirus) members.
Mode of transmission. seed dispersal, insect vectors spread and mechanical transmission.
SqMV For additional information, see Appendix A.
4 principle of the method
Squash mosaic virus serology, molecular characterization is the main basis for the development of this standard.
5 Equipment and Reagents
5.1 The main equipment and appliances
This standard is mainly related to the equipment and appliances are. juice extraction plant, mills, microplate reader, washer, microbalance (flu
Volume. 0.001g), PCR instrument, electrophoresis, horizontal electrophoresis tank gel imager, high-speed refrigerated centrifuge desktop, thermostat water bath or incubator,
pH meter, a variety of range adjustable pipette (1000μL, 200μL, 100μL, 20μL, 10μL, 2μL).
5.2 Reagents and preparation
Serological Reagents and buffer preparation see Appendix B, molecular biology reagents and buffer preparation see Appendices C and D.
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