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SN/T 4050-2014 PDF English

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SN/T 4050-2014: Quarantine Protocol for infection with abalone herpesvirus
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SN/T 4050-2014English389 Add to Cart 3 days [Need to translate] Quarantine Protocol for infection with abalone herpesvirus

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Basic data

Standard ID SN/T 4050-2014 (SN/T4050-2014)
Description (Translated English) Quarantine Protocol for infection with abalone herpesvirus
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 07.080
Word Count Estimation 15,138
Date of Issue 12/1/2014
Date of Implementation 5/1/2015
Quoted Standard GB/T 6682; GB/T 18088; GB 19489; SN/T 1193
Regulation (derived from) State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the clinical diagnosis, histopathology and molecular biology methods abalone herpes virus infection. This standard applies to abalone herpes virus infection diagnosis and monitoring.

SN/T 4050-2014: Quarantine Protocol for infection with abalone herpesvirus


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Abalone herpes virus infection quarantine technical specifications) People's Republic of China Entry-Exit Inspection and Quarantine Standards Abalone herpes virus infection quarantine Technical Specifications Issued on. 2014-11-19 2015-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shandong Entry-Exit Inspection and Quarantine Yantai People's Republic of China Bureau, the Shenzhen People's Republic of China Exit Inspection and Quarantine. The main drafters of this standard. Zhao Ran, Yin Wei force, Jiang Yuting, Zhengxiao Cong, Sun Mingjun, Zheng Xiaolong, He Junqiang, in force, Wang Jinjin, Yue Zhiqin. Abalone herpes virus infection quarantine Technical Specifications

1 Scope

This standard specifies the clinical diagnosis, histopathology and molecular biology methods abalone herpes virus infection. This standard applies to abalone herpes virus infection diagnosis and monitoring.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 18088 Entry and Exit Animal Quarantine sampling GB 19489 General requirements for laboratory biosafety SN/T 1193 genetic testing laboratory technical requirements

3 Abbreviations

The following abbreviations apply to this document. AbHV. abalone herpesvirus (Abaloneherpesvirus) BCIP. 5- bromo-4-chloro-3-indolyl - phosphate (5-Bromo-4-Chloro-3-IndolylPhosphate) Ct value. Threshold fluorescence signal reaches the set number of cycles experienced by amplification (Cyclethresholdvalue) EDTA. ethylenediaminetetraacetic acid (EthyleneDiamineTetraaceticAcid) NBT. nitroblue tetrazolium (NitrotetrazoliumBluechloride) PCR. polymerase chain reaction (Polymerasechainreaction) SDS. sodium dodecyl sulfate (Sodiumdodecylsulfate)

4 Reagents and materials

4.1 Water. in line with GB/T 6682 in the three water specifications. 4.2 hematoxylin. 4.3 eosin. 4.4 Primer. Table 1. Table 1 Primer sequences Name Sequence AbHVORF66F1 5'-TCC-CGG-ACA-CCA-GTA-AGA-AC-3 ' AbHVORF66R1 5'-CAA-GGC-TGC-TAT-GCG-TAT-GA-3 ' AbHV66Prb1 5'-6FAM-TGG-CCG-TCG-AGA-TGT-CCA-TG-TAMRA-3 ' ...

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