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SN/T 3843-2014: Determination of monascus pigment in foods for export
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SN/T 3843-2014: Determination of monascus pigment in foods for export

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Determination of monascus pigment in foods for export People's Republic of China Entry-Exit Inspection and Quarantine Standards Determination of food for export Monascus Pigment Issued on. 2014-01-13 2014-08-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Jilin People's Republic of China Exit Inspection and Quarantine. The main drafters of this standard. Ma Shumin, glory be, Zhang Xun, Mou Jun, ZHANG Dai Hui, Handa Chuan, Hu Tingting, Kangming Qin. Determination of food for export Monascus Pigment

1 Scope

This standard provides the food for export Monascus amine, monascorubrin, pigment red yeast, red yeast flavin liquid chromatography - mass spectrometry/mass spectrometry methods. Also it provides a HPLC method for the export of food in Monascus amine, Monascus Pigment. This standard applies to the preparation of food for export wine, ice cream, biscuits, ham, red yeast fermented bean curd in red amine, monascorubrin, pigment red yeast, red yeast Determination of flavin and confirmation.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 6682 analytical laboratory use specifications and test methods 3 Liquid chromatography - mass spectrometry/mass spectrometry 3.1 Method summary Ultrasonic liquid sample water extraction volume after centrifugation, solid and semi-solid samples were dissolved in water to volume, and then extract by solid phase extraction column Purification, after the sample solution by elution volume for liquid chromatography - mass spectrometry/mass spectrometer and validation, external standard. 3.2 Reagents and materials 3.2.1 Reagents and material requirements The method used reagents were HPLC grade water GB/T 6682 provisions of distilled water. 3.2.2 Reagents and materials Reagents and materials used in the method are as follows. --- Methanol. HPLC grade; --- Formic acid. chromatography; --- Acetonitrile. chromatography; --- Standard substance. Monascus amine (Fmoc-8-AOC-OH, CASN O.126631-93-4, C23H27NO4), monascorubrin (Monascusred, CASN O.874807-57-5, C23H26O5), Monascus pigment (monascin, CASN O.21516-68-7, C21H26O5), red yeast flavin (ankaflavin, CASN O.50980-32-0, C23H30O5) purity of greater than or equal to 97%; --- SPE. C18,1.0g, 6mL, or equivalent person. Before use 5mL methanol, 5mL water-activated; --- TF microporous membrane. 0.20μm. 3.2.3 preparation of 0.1% aqueous formic acid 1mL acid dissolved in 1000mL water. ...