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SN/T 3578-2013 PDF English

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SN/T 3578-2013	English	239	Add to Cart	3 days [Need to translate]	Viability detection of Fusarium virguliforme ODonnell et T.Aoki

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Basic data

Standard ID	SN/T 3578-2013 (SN/T3578-2013)
Description (Translated English)	Viability detection of Fusarium virguliforme ODonnell et T.Aoki
Sector / Industry	Commodity Inspection Standard (Recommended)
Word Count Estimation	6,628
Date of Issue	2013-03-01
Date of Implementation	2013-09-16
Regulation (derived from)	?AQSIQ About publishing 2013 First Batch 179-items Entry-Exit Inspection and Quarantine industry standard Announcement; Industry-Standard-Filing Announcement 2013 No. 9 (Total No. 165)
Issuing agency(ies)	General Administration of Customs
Summary	This standard specifies the application of laser scanning confocal microscope soybean sudden death of the North American species (Fusarium virguliforme O "Donnell et T. Aoki) activity carried out to detect the SARS virus. This standard applies to soybean

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SN/T 5268|

SN/T 3578-2013: Viability detection of Fusarium virguliforme ODonnell et T.Aoki

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Viability detection of Fusarium virguliforme O "Donnell et T.Aoki People's Republic of China Entry-Exit Inspection and Quarantine Standards Soybean Sudden Death Syndrome North American species activity detection method Issued on. 2013-03-01 2013-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Structure does not bear the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, Guangdong micro Institute of Biology, Chinese Academy of Inspection and Quarantine. The main drafters. Zhang Guiming, Wang Ying, Cheng Yinghui, Chen Chi-nan, Zhong Jianzhong, Li Qiufeng, only to Yu, on behalf of the Beijing Lufthansa, Wang Ying, Chen Hongjun. Soybean Sudden Death Syndrome North American species activity detection method

1 Scope

This standard specifies the application of laser scanning confocal microscopy North American soybean sudden death syndrome pathogen species (Fusariumvirguliforme O'DonneletT.Aoki) method for detecting the activity. This standard applies to the North American soybean sudden death syndrome pathogen related host species carried in the North American soybean sudden death syndrome pathogen species activity seized Test identification. Principle 2 Application of fluorescent dyes to the pathogen spores sickle dyeing treatment, according to the North American species of soybean sudden death syndrome, non-active bacteria Spores infected fluorescence, active spores are not infected with the different effects of fluorescence staining characteristics, the use of laser scanning confocal microscopy disease Spores were detected, analyzed the activity situation. 3 appliances, instruments and reagents 3.1 Instrument appliances Laser scanning confocal microscope, microscopes, autoclaves, biological safety cabinet, incubator, refrigerator. 3.2 Reagents Propidium iodide (PI), dimethyl sulfoxide (DMSO), agar powder.

4 activity test method

4.1 Get pathogen spores Under a microscope, you will find identified as North American soybean sudden death syndrome pathogen species sickle-shaped conidia pick sterilized water to prepare a concentration More than one spores/μL spore suspension, microscopy, spare. 4.2 Spore staining Take 1μL dye propidium iodide (out in DMSO at a concentration of 0.5mmol/L) was added 200μL spore suspension, mixing, Dyed dark at room temperature for 15min, 16000g supernatant centrifuged 1min termination staining, once sterilized deionized water, resuspended, concentrated spore Greater than 1 spores/μL. Dark for backup. 4.3 Laser scanning confocal microscope to detect activity 4.3.1 Sample preparation will be completed stained slides were mounted upside down on a laser scanning confocal microscope stage, low magnification to find the spores, Go 100x oil microscope, to fine-tune the image within the field of vision clear. 4.3.2 Select the appropriate laser tube (argon ion laser) fluorescence excitation signal, setting an excitation wavelength of fluorescence channels (488nm), collect fluorescent The emission wavelength of the optical signal (560nm), and set a bright field channel as a control. ...