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Chemicals. Fish assay for the reproduction toxicology
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Basic data
| Standard ID | SN/T 3524-2013 (SN/T3524-2013) |
| Description (Translated English) | Chemicals. Fish assay for the reproduction toxicology |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Word Count Estimation | 40,470 |
| Regulation (derived from) | AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the chemicals on fish reproductive toxicity testing methods described limitations, test principle, test acceptability criteria, methods are described, test design, test procedures, data and reports for the interpretation of the tes |
Similar standards
SN/T 5268|SN/T 3524-2013: Chemicals. Fish assay for the reproduction toxicology
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals. Fish assay for the reproduction toxicology
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Chemicals fish reproductive toxicity testing
Issued on. 2013-03-01
2013-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Table of Contents
Introduction Ⅲ
Introduction Ⅳ
1 Scope 1
2 Terms and definitions 1
Method 3 Limitations Description 1
4 Test Principle 2
5 test acceptability criteria 2
Method Description 3 6
6.1 Instrument 3
6.2 Water 3
6.3 Test Solution 3
6.4 4 fish breeding
6.5 Pre-exposure and selection of fish, 4
7 Test Design 4
7.1 General Description 4
7.2 Option 4 concentrations tested
8 Test procedure 5
8.1 Selection and weighing of test fish 5
8.2 Exposure Condition 5
8.2.1 Time 5
8.2.2 Feeding 5
8.2.3 lighting and temperature 5
8.3 Analysis of frequency detection and measurement of 5
8.4 Observation 5
8.4.1 General Description 5
8.4.2 viability 6
8.4.3 behavior and apparent 6
8.4.4 Fertility 6
8.4.5 fish humanely killed 6
8.4.6 Observation of secondary sexual characteristics 6
8.4.7 vitellogenin (VTG) 6
8.4.8 gonad histopathological evaluation 7
9 7 data and reports
9.1 using analysis of variance (ANOVA) to evaluate the response of biomarkers 7
9.2 Test results Report 7
10 For the interpretation of the test results and acceptance 8
Recommended sample program in Appendix A (informative) Analysis of vitellogenin collected 10
Annex B (informative) detection of specific endocrine active substances for fathead and secondary sexual characteristics of fish medaka assessment 19
Annex C (informative) fish endocrine screening tests experimental conditions 24
Annex D (informative) zebrafish and fathead minnow spawning substrate 26
Annex E (informative) acceptable dilution water some chemical characteristics of 28
Annex F (informative) vitellogenin strengthened between samples and reference standard analytical methods 29
Annex G (informative) Statistical analysis process 30
References 31
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard reference Organisation for Economic Co-operation and Development (OrganizationforEconomicCooperationandDevelopment,
OECD) TG229. "Fish short-term reproductive toxicity testing" (FishShortTermReproductionAssay) (in English).
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Cheng Yan, Cui Yuan, Falls Apart, Xie Wenping, Ma Ping.
Introduction
Ambient levels of chemical interaction with the endocrine system, crimes against humanity and wildlife, so the need to develop and validate detection within fish
Secretion interference techniques and methods of active substances. In 1998, OECD began the process of revising the existing test guidelines and the development of new testing guidelines,
To screen and detect potential endocrine disruptors. One of the important research is the development of test methods in fish endocrine disruptors guide.
Fish short-term toxicity tests Reproductive through extensive verification tests for selected chemicals across multiple laboratories phase method
Relevance and reliability tests, the selected chemicals through a variety of mechanisms including endocrine ways to influence fish reproduction [1] to [5].
The Test Guidelines (TG229) end all tests have been carried out to verify the fathead minnow, the end has been part of the Japanese medaka (such as egg yolk
Fibrinogen and secondary sexual characteristics) and zebrafish (such as vitellogenin) verified. Verify that part of the work group of countries by Test Method Guide
NCB (theNationalCoordinatorsoftheTestGuidelineProgramme) specified by the Group of Experts to complete [6], the other
Validation designated by the US Environmental Protection Agency [7] commissioned expert peer review. This test does not identify the specific mechanisms of hormonal disturbance, because
For the test animals with complete hypothalamic - pituitary - gonadal (HPG) axis, it will affect substance HPG axis have different degrees of reaction.
This test method describes a method for in vivo screening test, sexually mature males and females in the spawning period together, in their lives
Period of exposure to chemicals for a limited time (21d). In the 21d exposure period ends, depending on the species, the male and female fish
On for one or two biomarkers end test, as the test chemicals endocrine activity indicators. These tests endpoints included eggs
Huang fibrinogen and secondary sexual characteristics. For fathead minnow, Japanese medaka and zebrafish vitellogenin test can be carried out, but only in secondary sexual characteristics
Fathead minnow and Japanese medaka test on. Also throughout the testing process should be monitored daily quantitative fertility; gonad need to retain, may pass
Histopathological over animal testing to evaluate reproductive health, but also provide strong evidence for the other end of the test.
This biological testing as a screening test for specific endocrine disruptors in vivo model, its application in "OECD endocrine-disrupting chemicals
Testing and evaluation framework "to see. Within this framework, short-term fish reproductive test proposed for Level 4, as in vivo assays provide more
Species data endocrine disrupting mechanism.
Chemicals fish reproductive toxicity testing
1 Scope
This standard specifies the method for the chemical in fish reproductive toxicity test method described limitations, the test principle, test acceptability criteria,
The method described in the test design, test procedures, data and reports for the interpretation of the test results and acceptance.
This standard applies to chemicals in fish reproductive toxicity testing.
2 Terms and definitions
The following terms and definitions apply to this document.
2.1
Load factor loadingrate
Unit volume of water the fish wet weight.
2.2
Carrier density stockingdensity
The number of fish per volume of water.
2.3
Vitellogenin vitelogenin, VTG
Vitellogenin is a phospholipid glycoprotein precursor for forming a yolk protein, generally produced in all oviparous animals sexually mature female body.
2.4
The maximum tolerated concentration maximumtoleratedconcentration, MTC
The maximum tolerated concentration, represents about 10% of the LC50.
2.5
HPG axis hypothalamic-pituitary-gonadalaxis
Hypothalamic - pituitary - gonadal axis.
Method 3 Limitations Description
3.1 vitellogenin (Vitelogenin, VTG) normally circulating endogenous estrogen hormone stimulation that, by the female egg-laying vertebrates liver
produce. Yolk protein vitellogenin is a prerequisite, once produced in the liver by blood flow into the ovaries and developing female gamete suck
Income and change. Vitellogenin in immature female and male fish was barely detectable in plasma, because they lack sufficient circulating estrogen
Hormones, but the stimulation by exogenous estrogen, the liver can synthesize and secrete VTG.
3.2 VTG measurements can detect different modes of action of estrogen chemicals detected estrogenic chemicals, it is possible through
Measurement of vitellogenin in male fish produce, and there have been numerous reports [8] in the scientific literature counterparts. Also there is evidence showing vitellogenin
Produce can be caused by exposure to the aroma of androgen ([9] and [10]). Lowering the female body in circulating levels of estrogen, e.g., by
Inhibition of aromatase enzyme conversion of endogenous androgens as natural estrogen 17β- estradiol can cause reduced levels of vitellogenin, which is used
Having detected the enzyme aromatase inhibitory properties of chemical substances ([11] and [12]). We have established vitellogenin estrogen/aromatase inhibiting
The biological relevance of the response, and has been widely reported in the literature. However, in the female body VTG generated there may also be systemic toxicity
And non-toxic effects of endocrine disruptors affected models, such as liver toxicity.
...