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Rapid screen of clenbuterol residues with nano magnetic particles. Immunechromatographic assay
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Basic data
| Standard ID | SN/T 3502-2013 (SN/T3502-2013) |
| Description (Translated English) | Rapid screen of clenbuterol residues with nano magnetic particles. Immunechromatographic assay |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | X04 |
| Classification of International Standard | 67.050 |
| Word Count Estimation | 8,89 |
| Quoted Standard | GB/T 6682; GB/T 14699.1; GB/T 18088; SN/T 2123 |
| Regulation (derived from) | AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the use of nano-magnetic immunity chromatography, for animal food, feed and pig urine clenbuterol residues in the rapid screening test. This standard applies to foods of animal origin, feed and rapid screening of urine samples qual |
SN/T 3502-2013: Rapid screen of clenbuterol residues with nano magnetic particles. Immunechromatographic assay
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Rapid screen of clenbuterol residues with nano magnetic particles. Immunechromatographic assay
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Clenbuterol rapid detection of magnetic nanoparticles
Dipstick
Issued on. 2013-03-01
2013-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions should not bear the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, Tsinghua University
Shenzhen Graduate School, Shenzhen Key Laboratory of harmful alien biomass detection technology development.
The main drafters of this standard. Qin Zhifeng, body Lu Kang, Sun Jie, Ma Lan, Lv Jianjiang, flower Qun Yi, Chenshu Kun, had less spirit, Lin Qingyan, Caochen Fu,
Wu Feng, Bing, Ruanzhou Xi, Yangjun Xing, Tao Hong, Hang Yuan, Zhang Caihong, Zong Hui, Liu Jianli, Liao Lishan.
Clenbuterol rapid detection of magnetic nanoparticles
Dipstick
1 Scope
This standard specifies the use of nano-magnetic immunity chromatography, foods of animal origin, feed and pig urine clenbuterol residues in
Rapid screening tests.
This standard applies to foods of animal origin, feed and urine samples in the rapid screening of Clenbuterol qualitative detection.
The standard detection limit of 0.1μg/kg.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 14699.1 feed samples
GB/T 18088 Entry and Exit Animal Quarantine sampling
SN/T 2123 Entry and Exit Animal Quarantine experimental sample collection, transport and storage specifications
Principle 3
In this method, immunochromatography the competitive inhibition method, its main principle is. by bovine serum albumin conjugated anti-clenbuterol
Original, anti-mouse IgG antibody, nano superparamagnetic-labeled anti-clenbuterol antibody and other reagents fixed nitrocellulose membrane, glass fiber
Test strip made of paper on plain film membranes, apply the principles of competition law formula chromatography sample is detected clenbuterol residues. Samples salt
Clenbuterol acid in the process of flowing nano-cellulose membrane with glass beads labeled specific anti-clenbuterol antibody binding, inhibition
Antibody test line and a nitrocellulose membrane BSA - clenbuterol conjugate antigen binding, binding to the detection line is satisfied
Reduce the amount of rice beads, using a magnetometer and the sample values measured magnetic strip interpretation detection line to get in the clenbuterol content into
Inversely proportional.
4 Reagents and Instruments
4.1 Reagents
4.1.1 reagent grade
Except as otherwise provided, the method should meet the two test water water GB/T 6682 provisions, the use of chemical reagents were of analytical grade.
4.1.2 Clenbuterol nanometer magnetic immunochromatographic rapid test strip.
4.1.3 rinse (see Appendix A Formulation of A.1).
4.1.4 0.1mol/L hydrochloric acid solution.
4.1.5 1.0mol/L sodium hydroxide solution.
4.1.6 pH7.40.02mol/LPBS solution (see recipe A.2).
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