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Quarantine protocol for Gnathostoma in aquatic products
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Basic data
| Standard ID | SN/T 3497-2013 (SN/T3497-2013) |
| Description (Translated English) | Quarantine protocol for Gnathostoma in aquatic products |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Word Count Estimation | 13,136 |
| Quoted Standard | GB/T 6682; GB/T 18088 |
| Regulation (derived from) | ?AQSIQ About publishing 2013 First Batch 179-items Entry-Exit Inspection and Quarantine industry standard Announcement; Industry-Standard-Filing Announcement 2013 No. 9 (Total No. 165) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the morphological identification and PCR detection methods Gnathostoma third stage larvae of nematodes. This standard applies to fish in the jaw mouth nematode quarantine, epidemiological investigation and monitoring. |
Similar standards
SN/T 5268|SN/T 3497-2013: Quarantine protocol for Gnathostoma in aquatic products
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for Gnathostoma in aquatic products
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Aquatic Products jaw spinigerum quarantine technical specifications
Issued on. 2013-03-01
2013-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Guangxi University, Guangxi Zhuang Autonomous Region Disease Prevention and Control
System Center, Chinese Center for Disease Control Parasitic Diseases Control and Prevention, Heilongjiang People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Li Shuqing, Huang Weiyi, Hong-Man, Chen Shao Hong, Li Wenwen, Wu Huifang, Li Jian, Zhang, Zhang subgroup,
Zhifei Chen, Wang Qiao whole, Zhang Qiang, Wang Yan.
Aquatic Products jaw spinigerum quarantine technical specifications
1 Scope
This standard specifies the morphological analysis and PCR detection method jaw spinigerum third stage larvae.
This standard applies to fish in the jaw spinigerum quarantine, epidemiological investigation and monitoring.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 Overview
Jaw nematode (Gnathostomiasis) is a human beast from the jaw spinigerum (Gnathostoma) caused by parasitic disease. Mainly due to people
Ingestion of raw or undercooked containing jaw spinigerum third stage larvae of aquatic worm disease caused by jaw mouth. Jaw spinigerum classification, life history, points
Cloth See Appendix A.
4 Equipment and Materials
4.1 Instruments and equipment
4.1.1 Biological microscope.
4.1.2 stereomicroscope.
4.1.3 PCR amplification.
4.1.4 electrophoresis.
4.1.5 gel imaging system.
4.1.6 mesh sieve (10 mesh).
4.2 Reagents and solutions
4.2.1 Taq enzyme (5U/μL).
4.2.2 2.5mmol/LdNTP.
4.2.3 25mmol/LMgCl2.
4.2.4 10 × PCR buffer (without Mg2).
4.2.5 Molecular Weight indicator. 100bp ~ 2000bpDNAmarker.
4.2.6 50% and 70% ethanol.
4.2.7 water, in line with GB/T 6682 stipulated a water.
4.2.8 Artificial digestion, phenol transparent lactate solution, Tris-acetate electrophoresis buffer (the TAE), agarose gel, 6 × loading buffer (preparation
See Appendix B).
4.3 Primer
Amplification jaw spinigerum ribosomal DNA second internal transcribed spacer (ITS2) primer sequences are.
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