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Protocol of the real-time PCR for detecting genetically modified component in animal-originated-feedstuffs
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Basic data
| Standard ID | SN/T 3496-2013 (SN/T3496-2013) |
| Description (Translated English) | Protocol of the real-time PCR for detecting genetically modified component in animal-originated-feedstuffs |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 12,192 |
| Quoted Standard | GB/T 6682 |
| Regulation (derived from) | AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the method for real-time PCR test GMO animal feed. This standard applies to pigs, cattle, sheep genetically modified animal feed real-time PCR test. |
SN/T 3496-2013: Protocol of the real-time PCR for detecting genetically modified component in animal-originated-feedstuffs
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Protocol of the real-time PCR for detecting genetically modified component in animal-originated-feedstuffs
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Genetically modified animal feed real-time fluorescence
PCR detection method
Issued on. 2013-03-01
2013-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Anhui Exit Inspection and Quarantine, the People's Republic of China
And country SZCIQ, China Agricultural University, Beijing Animal Husbandry and Veterinary.
The main drafters of this standard. Liu, Linxiang Mei, ZHU Zhen camp, Zhang Yongning, Jianwu, Wu Shaojiang, Zhenghai Song Ling apricot, Xue Zhenhua, Zhang Min,
Yu Ning, Caixia, Li Zhihui, Li Jinghong.
Genetically modified animal feed real-time fluorescence
PCR detection method
1 Scope
This standard specifies the method for real-time PCR testing of genetically modified animal feed.
This standard applies to pigs, cattle, sheep, animal feed GMO real time PCR test.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Abbreviations
The following abbreviations apply to this document.
β2m. β2-microglobulin (β2-microglobulin)
bp. base pairs (basepair)
CMV. CMV (cytomegalovirus)
Ct. each reaction cycle number (cyclethreshold) fluorescence signal within the tube reaches the set threshold experienced
dATP. triphosphate (deoxyadenosinetriphosphate)
dCTP. deoxycytidine triphosphate (deoxycyticinetriphosphate)
dGTP. deoxyguanosine triphosphate (deoxyguanosinetriphosphate)
dNTP. deoxynucleotide triphosphate (deoxyribonucleosidetriphosphate)
dTTP. deoxythymidine triphosphate (deoxythymidinetriphosphate)
DNA. deoxyribonucleic acid (deoxyribonucleicacid).
GFP. green fluorescent protein (greenfluorescentprotein)
NPTⅡ. neomycin 3'-phosphotransferase gene (neomycin-3'-phosphotransferasegene)
PCR. polymerase chain reaction (polymerasechainreaction)
SV40. simian virus 40 (SimianVacuolatingvirus40)
Taq DNA. DNA polymerase.
TE. Tris-HCl, EDTA buffer
Tris. Tris (hydroxymethyl) aminomethane (trishydroxymethylaminomethane)
4 Experimental Method
4.1 The main instruments
4.1.1 Real-Time PCR instrument.
4.1.2 clean bench.
4.1.3 Sterilization pot.
4.1.4 Vortex.
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