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Detection and identification of potato spindle tuber viroid
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Basic data
| Standard ID | SN/T 3437-2012 (SN/T3437-2012) |
| Description (Translated English) | Detection and identification of potato spindle tuber viroid |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Word Count Estimation | 17,182 |
| Quoted Standard | GB/T 6682; SN/T 2122 |
| Regulation (derived from) | National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the Potato spindle tuber viroid (potato spindle tuber viroid) quarantine identification. This standard applies to detect possible with potato spindle tuber viroid Potato seedlings, plantlets, potato, potato products, field-grown an |
Similar standards
SN/T 5268|SN/T 3437-2012: Detection and identification of potato spindle tuber viroid
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of potato spindle tuber viroid
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Potato spindle tuber viroid identification of quarantine
Issued on. 2012-12-12
2013-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Exit Inspection and Quarantine of Heilongjiang People's Republic of China, People's Republic of China Xiamen Entry-Exit Inspection and Quarantine
Bureau, People's Republic of China Hubei Exit Inspection and Quarantine, Heilongjiang Academy of Agricultural Sciences Institute of Plant Virus-free seedlings.
The main drafters of this standard. Liu Hongyi, Liu Zhongmei, Chen Yun, Lu Dian autumn Qiucai Ling, Zhang Hongxiang, Wang Zhenhua, Yang Liqun, Liu Shangwu.
Potato spindle tuber viroid identification of quarantine
1 Scope
This standard specifies the potato spindle tuber viroid (potatospindletuberviroid) of quarantine and identification methods.
This standard applies to detect possible with potato spindle tuber viroid potato virus-free seedlings, plantlets, seed potatoes, potato products, in Daejeon
Growth of other hosts and post-harvest potato tubers, tomatoes, avocado, ginseng fruits.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
SN/T 2122 entry and exit quarantine of plants and plant products sampled
Principle 3
3.1 Potato spindle tuber viroid basic information
Species. potatospindletuberviroid
Abbreviation. PSTVd
Synonyms. potatospindletubervirus, potatogothicvirus, tomatobunchytopvirus.
Category. Potato spindle tuber viroid Branch (familypospiviroidae), potato spindle tuber viroid genus (GenusPospivir-
oid).
For additional information, see Appendix Potato spindle tuber viroid A.
3.2 based quarantine and identification
Genomic features and symptoms characteristic PSTVd in the host plant is the basis for identification of quarantine. PSTVd is a kind of infection
, Non-coat protein, highly base-paired rod-like single-stranded covalently closed circular RNA molecules small. After the transition of RNA denaturation PSTVd
To open cyclic molecules, RNA molecules such cyclic polyacrylamide gel electrophoresis mobility than the same molecular weight linear points
Sub much slower. Based on the establishment of genomic features a roundtrip polyacrylamide gel electrophoresis (Return-PolyacrylamideGelElectropho-
resis, R-PAGE), RT-PCR, real-time fluorescence RT-PCR (Real-timePCR) detection methods that combine host plants symptoms
The presence or absence of symptoms and characteristics (see Appendix A), determine whether the sample with PSTVd.
4 equipment, appliances, and reagents
4.1 Equipment
PCR, real-time PCR instrument, electrophoresis, horizontal electrophoresis tank, vertical electrophoresis tank, a small centrifuge, desktop refrigerated centrifuge, water bath
Pot, gel imaging system, ice maker, refrigerator freezer, electronic scales, a vortex, oven.
...
