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Detection and identification of sowbane mosaic virus
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Basic data
| Standard ID | SN/T 3436-2012 (SN/T3436-2012) |
| Description (Translated English) | Detection and identification of sowbane mosaic virus |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Word Count Estimation | 12,185 |
| Regulation (derived from) | National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the Li flower mosaic virus quarantine and identification of serological and molecular detection methods. This standard applies may carry the virus leaves Li flower seeds, seedlings and plant quarantine and identification of product |
Similar standards
SN/T 5268|SN/T 3436-2012: Detection and identification of sowbane mosaic virus
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of sowbane mosaic virus
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Quinoa leaf flower quarantine and identification of viruses
Issued on. 2012-12-12
2013-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Chen Qing, MATURAL, Huang Feng, Chen Yun, Liao Furong, Lin Shiming.
Quinoa leaf flower quarantine and identification of viruses
1 Scope
This standard specifies the serological and molecular biology methods quinoa flower leaf virus quarantine identified.
This standard applies to seed of a plant quarantine and identification of possible virus with pigweed flowers leaves, seedlings and plant products.
Principle 2
Name. sowbanemosaicvirus
Abbreviation. SoMV
Taxonomic position. Southern bean mosaic virus genus (sobemovirus) members.
Antigenic properties and characteristics of the genome is the main basis for quarantine and identification of.
For more information, see Appendix A.
3 instruments, equipment and reagents
3.1 equipment and appliances
Trace Mills, microplate reader, washer, microbalance (sense of volume. 0.001g), PCR instrument, electrophoresis, horizontal electrophoresis tank, gel imaging
Instrument, desktop high-speed refrigerated centrifuge, water bath, pH meter, adjustable pipette (1000μL, 200μL, 20μL, 2μL), 96 well microtiter plates, RESEARCH
Beryllium; pest control greenhouse.
3.2 Reagents
Reagents and buffers see Appendices B and C.
Preparation of test sample 4
4.1 Preparation of seed samples
Picked malformation, immature seeds were sown in sterilized soil, grow to be 3 to 4 leaves of plants will show symptoms numbers do not show symptoms of the plant
Strain grouping (10 per group) and numbered, collected leaves into 2 parts, respectively, as required for enzyme-linked assays and molecular biology.
Can also be picked malformation, immature seeds directly enzyme-linked assays and molecular biology.
4.2 seedlings
Symptoms (such as blade malformation, mosaic, mottled, etc.) number of seedlings individually tested. No symptoms of the grouping and number detection, grouping
And detection methods with 4.1.
4.3 Plant Products
Plant products are part of the symptoms (such as. blades malformation, mosaic, mottled, etc.) individually numbered test. No signs or symptoms can not be observed
Plant products, shall be proportional sampling group number, grouping method and detection methods with 4.1.
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