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Detection and identification of Diaporthe helianthi Muntanola-Cvetkovic
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Basic data
| Standard ID | SN/T 3432-2012 (SN/T3432-2012) |
| Description (Translated English) | Detection and identification of Diaporthe helianthi Muntanola-Cvetkovic |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Word Count Estimation | 8,872 |
| Quoted Standard | GB/T 6682; SN/T 1538.1; SN/T 1809 |
| Regulation (derived from) | National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the sunflower stem canker Isolation and molecular identification methods. This standard applies to entry and exit of sunflower seeds and sunflower field sunflower plants stem canker quarantine and identification. |
Similar standards
SN/T 5268|SN/T 3432-2012: Detection and identification of Diaporthe helianthi Muntanola-Cvetkovic
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Detection and identification of Diaporthe helianthi Muntanola-Cvetkovic
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Sunflower stem canker quarantine and identification methods
Issued on. 2012-12-12
2013-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Xinjiang People's Republic of China Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Wang Wen-cheng, Zhang Xianglin, Du Hongzhong Li Yan, Zhangjiang Guo.
Sunflower stem canker quarantine and identification methods
1 Scope
This standard specifies the sunflower stem canker pathogen isolation, culture and identification of molecular biology.
This standard applies to the entry and exit of sunflower seeds and sunflower field sunflower plants stem canker quarantine and identification of bacteria.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
SN/T 1538.1 media preparation Guide - Part 1. General laboratory quality assurance prepared medium
SN/T 1809 export plant quarantine rules for seed
3 sunflower stem canker basic information
Name. Sexuality. DiaporthehelianthiMuntanola-CvetkovicMihaljcevicetPetrov;
Anamorph. PhomopsishelianthiMunt.-Cvetk, Mihaljc..
Taxonomic position. Sexuality is Ascomycotina (Ascomycotina), Pyrenomycetes (Pyrenomycetes), spherical shell mesh (Sphaeriales),
Zama Shell Branch (Diaporthaceae), between the seat shell genus (Diaporthe); anamorph of imperfect fungi Amon (Deuteromycotina), Coelomycetes
(Coelomycetes), Sphaeropsidales (Sphaeropsidales), spherical shell spore Branch (Sphaeropsidaceae), Phomopsis genus (Phomopsis).
Transmission. The germs spread mainly by close range by means of spores splashing rain, plant debris and operating tool for the dissemination of farming, remote
From spread mainly through contaminated sunflower seeds and seeds transported entrained in sick deformed spread.
Other relevant information, see Appendix A. The sunflower stem canker
Principle 4 Identification
According sunflower stem canker infestation symptoms on the host, morphology, culture characteristics and specificity of PCR amplified fragment as
Identification basis.
5 apparatus and appliances
5.1 Instrument
Stereo microscope, biological microscope, clean bench, PCR amplification device, electrophoresis, gel imager, light incubator, electronic scales
(1/1000g), autoclave, fridge.
5.2 Appliances
Hand-held magnifying glass, dish, alcohol lamp, tweezers, scissors, scalpels, sample sieve (10 mesh, φ1.7mm), pruning shears, inoculating needle, slide glass
Sheet, cover glass, sealed membrane, Culture Collection tubes, centrifuge tubes (1.5mL), adjustable and supporting micro pipette tips (10μL ~ 1000μL).
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