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Determination of β-lactams and tetracyclines residues in export milk. ROSA method
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Basic data
| Standard ID | SN/T 3256-2012 (SN/T3256-2012) |
| Description (Translated English) | Determination of ��-lactams and tetracyclines residues in export milk. ROSA method |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Word Count Estimation | 16,110 |
| Quoted Standard | GB/T 6682 |
| Regulation (derived from) | National Quality Inspection (2012) 643 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the export of milk ��- lactams and tetracyclines residues ROSA screening detection methods. This standard applies to exports of milk ��- lactams (including penicillin G, amoxicillin, ampicillin, oxacillin, cloxacillin, dicloxacillin, |
Similar standards
SN/T 5268|SN/T 3256-2012: Determination of β-lactams and tetracyclines residues in export milk. ROSA method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of β-lactams and tetracyclines residues in export milk.ROSA method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
β- lactam export milk and tetracycline
The residue rapid detection method ROSA
ROSAmethod
Issued on. 2012-10-23
2013-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Fujian Exit Inspection and Quarantine.
The main drafters of this standard. Lin Jie, Huang Xiaorong, Zheng Jing, Huang Chang Jiao, Chen Bin, sheets of silver, Jiangshu Xun, Chen Jian.
β- lactam export milk and tetracycline
The residue rapid detection method ROSA
1 Scope
This standard specifies the β- lactam export milk and ROSA screening method for detecting residues of tetracyclines.
This standard is applicable to the export β- lactams in milk (including penicillin G, amoxicillin, ampicillin, oxacillin, cloxacillin,
Dicloxacillin, cephalexin, cefazolin, ceftiofur) and tetracyclines (including tetracycline, chlortetracycline, oxytetracycline) qualitative drug residues
Rapid screening assay.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
Principle 3
ROSA detection method is a method for the determination of drug residues ROSA application analyzer, which is based on colloidal gold immunoassay. Reagents
Article colloidal gold conjugate pad package microorganism receptor antibody colloidal gold complexes, testing lines are individually coated with penicillin G antigens and antigen chlortetracycline,
Control line coated microbial receptors secondary antibodies. When the sample contains β- lactams and tetracyclines, and will test line coating antigen competition
Combined gold complexes, gold complexes and detection line coating antigen binding form a red line, not the gold standard composite detect bound antigen-coated wire
It was moved to the control line and a second antibody to form a red line. Detecting the intensity of the sample line contained in the color is inversely proportional to the concentration of the drug, the test line
Lighter than the control line is positive; the test line is deeper than or equal to the control line is negative; incomplete or no color control line as invalid test.
4 Reagents
Determination of residues in milk β- lactams and tetracyclines kit (see Appendix A).
5 Equipment and Materials
5.1 ROSA analyzer.
5.2 ROSA incubator. 56 ℃ ± 1 ℃.
5.3 Refrigerator. 2 ℃ ~ 8 ℃.
5.4 pipette. 300μL.
6 Sample Storage
Milk in the refrigerator 4 ℃ ± 1 ℃ refrigerated.
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