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Quarantine protocol of RT-PCR for swine getah disease
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Basic data
| Standard ID | SN/T 3198-2012 (SN/T3198-2012) |
| Description (Translated English) | Quarantine protocol of RT-PCR for swine getah disease |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Word Count Estimation | 7,760 |
| Quoted Standard | GB/T 6682 |
| Regulation (derived from) | State inspection recognized (2012) 643; industry standard filing Notice 2013 No. 4 (No. 160 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the pig qata disease quarantine norms. This standard applies to pig qata epidemiological investigations and Inspection and Quarantine. |
Similar standards
SN/T 5268|SN/T 3198-2012: Quarantine protocol of RT-PCR for swine getah disease
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol of RT-PCR for swine getah disease
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Swine Gaeta disease by RT-PCR Technical Specifications
Issued on. 2012-10-23
2013-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing, People's Republic of China Entry-Exit Inspection and Quarantine
Bureau, Nanjing Agricultural University.
The main drafters of this standard. Wangkai Min, Zhao Xiaoyan, Jiang Yan, Hou Yufeng, high-Jane Lee, Yu Bing, Liu Jun, Tang Tai Shan, Chen Guoqiang, Zhou Bin,
Zhang often printed.
Swine Gaeta disease by RT-PCR Technical Specifications
1 Scope
This standard specifies the swine Gaita disease quarantine norms.
This standard applies to pig Gaita Epidemiological Investigation and Inspection and Quarantine.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Abbreviations
The following abbreviations apply to this document.
Getah. Gaeta disease
4 Reagents and materials
4.1 Reagents
4.1.1 TAE electrophoresis buffer preparation see Appendix A.1,1.0% agarose gel plate preparation see Appendix A.2.
4.1.2 synthetic pig disease virus tower cover CAP gene as a positive control, sterilized DEPC-treated distilled water as negative
Control.
4.1.3 primer.
Primer 1 (20 pmol/μL) CAP-1. 5'-cgcctcgagatgaattacattccaactcaa-3 '
Primer 2 (20 pmol/μL) CAP-2. 5'-ctcctgcagccattcttctgttccttctgg-3 '
4.1.4 RT-PCR reagents. PCR reaction buffer, AMV reverse transcriptase, RNaseinhibitor and Taq enzyme, dNTPs,
MgCl2, DEPC treated sterile distilled water.
4.1.5 Electrophoresis loading buffer. 6 × loadingbuffer.
4.1.6 DNA molecular weight markers. 100bp ~ 2000bp.
4.2 Material
4.2.1 PCR thermal cycler.
4.2.2 electrophoresis.
4.2.3 gel imaging system.
5 Operation
5.1 Sample Preparation
5.1.1 mosquito sample
Take mosquito to be tested with DEPC-treated water and the overall configuration of PBS slurry mixing ratio of 1.10 into the grinder in an ice bath
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