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Determination of aflatoxins, ochratoxin, fumonisin B1, deoxynivalenol, T-2 and HT-2 toxins in peanut, grain and their products for export
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Basic data
| Standard ID | SN/T 3136-2012 (SN/T3136-2012) |
| Description (Translated English) | Determination of aflatoxins, ochratoxin, fumonisin B1, deoxynivalenol, T-2 and HT-2 toxins in peanut, grain and their products for export |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | X11 |
| Classification of International Standard | 67.060 |
| Word Count Estimation | 36,364 |
| Regulation (derived from) | National Quality Inspection (2012) 237 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the export peanuts, grains and their products of aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisin B1, Deoxynivalenol DON, T-2 toxin, HT-2 toxin Determination liquid chromatography mass spectrometry/mass spectrometry. This standar |
SN/T 3136-2012: Determination of aflatoxins, ochratoxin, fumonisin B1, deoxynivalenol, T-2 and HT-2 toxins in peanut, grain and their products for export
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of aflatoxins, ochratoxin, fumonisin B1, deoxynivalenol, T-2 and HT-2 toxins in peanut, grain and their products for export
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Export peanuts, grains and their products aflatoxin,
Ochratoxin, fumonisin B1, deoxynivalenol
Bacteria enolase, T-2 toxin, HT-2 toxin assay
Determinationofaflatoxins, ochratoxin, fumonisinB1, deoxynivalenol,
T-2andHT-2toxinsinpeanut, grainandtheirproductsforexport
Issued on. 2012-05-07
2012-11-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Entry-Exit Inspection and Shanxi
Quarantine Bureau.
The main drafters of this standard. Zhu Zhen Yi, Song Huan, Feng people, even GY, Yan Weidong, flowers Kam, He Jian, Zhao Xiaotian.
Export peanuts, grains and their products aflatoxin,
Ochratoxin, fumonisin B1, deoxynivalenol
Bacteria enolase, T-2 toxin, HT-2 toxin assay
1 Scope
This standard specifies the export peanuts, cereals and products of aflatoxin B1, B2, G1, G2, ochratoxin A, fumonisin B1,
Deoxynivalenol fungi alcohol, T-2 toxin, HT-2 toxin assay --- liquid chromatography - mass spectrometry/mass spectrometry methods.
This standard is applicable to the export peanuts, cereals and products of aflatoxin B1, B2, G1, G2, ochratoxin A, fumonisin B1,
Deoxynivalenol fungi alcohol, T-2 toxin and HT-2 toxin assay.
2 sample preparation
2.1 solid sample preparation
The sample was reduced by quartering to 1kg, all ground and ground to a particle size of less than 2mm, mixing, are divided into two as a sample, respectively
Inside into a clean container, sealed and labeled. In the course of sampling and sample preparation operation, should prevent sample contamination or the occurrence of residues
Change of.
2.2 Sample preservation
The sample was stored in the dark at 4 ℃.
3 Method summary
The sample was PBS and methanol - aqueous extract, the extract was diluted, filtered and purified by immunoaffinity column by rinsing to remove Free
Immunoaffinity column impurities, followed by an eluent through the column, the target is separated off, dry nitrogen volume. Liquid chromatography - mass spectrometry/mass spectrometry measurement
Fixed, external standard.
4 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water for ultra-pure water.
4.1 Methanol. HPLC grade.
4.2 acetonitrile. high performance liquid chromatography.
4.3 toluene.
4.4 formic acid.
4.5 ammonium acetate.
4.6 Disodium hydrogen phosphate (Na2HPO4 · 12H2O).
4.7 potassium chloride.
4.8 potassium dihydrogen phosphate.
4.9 sodium chloride.
...
