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SN/T 2976-2011 PDF English

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SN/T 2976-2011: Quarantine protocol for piscirickettsia salmonis by nested polymerase chain reaction
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SN/T 2976-2011English239 Add to Cart 3 days [Need to translate] Quarantine protocol for piscirickettsia salmonis by nested polymerase chain reaction

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Basic data

Standard ID SN/T 2976-2011 (SN/T2976-2011)
Description (Translated English) Quarantine protocol for piscirickettsia salmonis by nested polymerase chain reaction
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 07.080
Word Count Estimation 9,913
Date of Issue 2011-09-09
Date of Implementation 2012-04-01
Quoted Standard GB/T 6682
Adopted Standard OIE Aquatic Animal Disease Diagnostic Manual -2006, NEQ
Regulation (derived from) National Quality Inspection (2011) 492; industry standard filing Notice 2012 No. 4 (No. 148 overall)
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the salmon rickettsial nested polymerase chain reaction detection methods. This standard applies to salmon rickettsial quarantine.

SN/T 2976-2011: Quarantine protocol for piscirickettsia salmonis by nested polymerase chain reaction




---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for piscirickettsia salmonis by nested polymerase chain reaction People's Republic of China Entry-Exit Inspection and Quarantine Standards Salmon rickettsia quarantine Technical Specifications Nested polymerase chain reaction Issued on. 2011-09-09 2012-04-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard and the International Organization for Animal Health (OIE) "aquatic animal disease diagnostic manual" (2006 edition) the first chapter 2.1.13 degree of consistency Not equivalent. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine Bureau Technology Center, Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine Beijing People's Republic of China. The main drafters of this standard. Xie star, Peng Xiaoli, Liu Tong, Chen Shuangya, Orientin Liu, Jia Peng, Zhang Lifeng, THE STAFF, Wang Qun force, Wang Jingming. Salmon rickettsia quarantine Technical Specifications Nested polymerase chain reaction

1 Scope

This standard specifies the salmon rickettsial nested polymerase chain reaction detection method. This standard applies to salmon rickettsial quarantine.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Abbreviations

The following abbreviations apply to this document. bp (basepair). bp DNA (deoxyribonucleicacid). DNA dNTP (deoxyribonucleosidetriphosphate). deoxynucleotide triphosphate EDTA (ethylenediaminetetraaceticacid). ethylenediaminetetraacetic acid Nested-PCR (nestedpolymerasechainreaction). nested polymerase chain reaction SDS (sodiumdodecylsulfate). sodium dodecyl sulfate Taq (Thermusaquaticu). aquatic habitat heat strain TE. Tris-Cl, EDTA buffer Tris [tris (hydroxymethyl) aminomethane]. tris (hydroxymethyl) aminomethane

4 Equipment and Reagents

4.1 Device 4.1.1 -20 ℃ cryogenic refrigerator. 4.1.2 tissue homogenizer. 4.1.3 High-speed refrigerated centrifuge. 4.1.4 biological safety cabinet. 4.1.5 incubator. 4.1.6 PCR instrument. 4.1.7 electrophoresis. 4.1.8 gel imager or ultraviolet light observation. ...

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