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SN/T 2867-2011: Qualitative identification of fish derived material in feedstuff. PCR method
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SN/T 2867-2011: Qualitative identification of fish derived material in feedstuff. PCR method

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Qualitative identification of fish derived material in feedstuff.PCR method People's Republic of China Entry-Exit Inspection and Quarantine Standards Fish derived materials in feed detection method PCR methods PCRmethod Issued on. 2011-05-31 2011-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau. The main drafters. Zhang Shuya, bear Wei, Jiang Jing, Pan Liangwen. Fish derived materials in feed detection method PCR methods

1 Scope

This standard specifies the method of PCR-derived ingredients fish feed detection, detection limit of the assay was 0.1%. This standard applies to qualitative detection of fish feed-derived ingredients.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 14699.1 feed samples SN/T 1193 genetic testing laboratory technical requirements

3 Abbreviations

PCR (polymerasechainreaction). Polymerase chain reaction DNA (deoxyribonudeicacid). DNA Principle 4 Use of cell lysate broken, chloroform extraction of proteins, ethanol precipitation to give DNA; with the extracted DNA as a template PCR amplification and agarose gel electrophoresis to detect PCR amplification products; positive PCR product was sequenced application for confirmation.

5 Reagents and materials

Unless otherwise specified, all reagents were of analytical grade or biochemical reagents. Test water line with GB/T 6682 requirements. All reagents were Pollution-free DNA enzyme dispensing container. 5.1 detection primer comprising. a) Forward primer. 5'-TAAgAgggCCggTAAAACTC-3 ' b) reverse primer. 5'-gTggggTATCTAATCCCAg-3 ' 5.2 cetyl trimethyl ammonium bromide (CTAB). 5.3 Tris (Tris). 5.4 ethylenediaminetetraacetic acid (EDTA). 5.5 Sodium chloride (NaCl). 5.6 chloroform (chloroform). 5.7 isopropanol. ...