SN/T 2734-2020 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2734-2020	RFQ	ASK	3 days	(Technical specifications for quarantine of infectious salmon anemia)	Valid
SN/T 2734-2010	679	Add to Cart	3 days	Quarantine protocol for infectious salmon anaemia (ISA)	Obsolete

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Basic data

Standard ID: SN/T 2734-2020 (SN/T2734-2020)
Description (Translated English): (Technical specifications for quarantine of infectious salmon anemia)
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 07.080
Date of Issue: 2020-12-30
Date of Implementation: 2021-07-01
Older Standard (superseded by this standard): SN/T 2734-2010
Regulation (derived from): General Administration of Customs Announcement No. 136 [2020]
Issuing agency(ies): General Administration of Customs

SN/T 2734-2010: Quarantine protocol for infectious salmon anaemia (ISA)

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for infectious salmon anaemia (ISA) People's Republic of China Entry-Exit Inspection and Quarantine Standards Infectious salmon anemia quarantine Technical Specifications Issued on. 2010-11-01 2011-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is a reference to the World Organisation for Animal Health (OIE) "aquatic animal disease diagnostic manual)) (2009 edition) in chapter 2.3.5 concerning Content, while developing evidence base made abroad in the field of research achievements on. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine, Shenzhen People's Republic of China Shenyang People's Republic of China and Exit Inspection and Quarantine. The main drafters of this standard. German Fan, Xu Shu Fei, Liu Orientin high Lung Ying, Lin Ying, Chen Xinzhong, Gong Yanqing. Infectious salmon anemia quarantine Technical Specifications

1 Scope

This standard specifies the clinical diagnosis for the detection of infectious salmon anemia virus isolation, indirect fluorescent antibody test, RT-PCR Party And fluorescence quantitative RT-PCR methods. This standard applies to the epidemiological investigation of infectious salmon anemia, monitoring, diagnosis and exit inspection and quarantine.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Abbreviations

The following abbreviations apply to this document. ASK (atlanticsalmonkidneycelline). Atlantic salmon kidney cell line CHSE-214 (chinooksalmonembryoniccelline). chinook salmon fish embryonic cell lines CPE (cytopathiceffect). pathological changes in cells DABCO (1,4-Diazabicyclo [2.2.2] octane). triethylenediamine, also known as 1,4-diazabicyclooctane DEPC (diethypyrocarbonate). diethylpyrocarbonate EB (ethidiumbromide). ethidium bromide FITC (fluoesceinisothiocyanate). fluorescein isothiocyanate IFAT (indirectfluorescentantibodytest). indirect fluorescent antibody test ISA (infectioussalmonanaemia). infectious salmon anemia M-MLV reverse transcriptase (moloneymurineleukemiavirus). isolated from Moloney murine leukemia virus SHK-1 (atlanticsalmonheadkidney). Atlantic salmon before kidney cell line

4 Reagents and materials

Standard 4.1 ISAV strains, ISAV monoclonal antibody, rabbit anti-ISAV antibodies, goat anti-mouse IgG is fluorescently labeled, fluorescent-labeled goat anti-rabbit IgG, cell line (SHK-1, TO, ASK or CHSE-214 cells); laboratories designated by the State Administration of Quality Supervision, Inspection and Quarantine. 4.2 Water. reagent preparation (see Appendix A) water should comply with GB/T 6682 in a water specifications. 4.3 TaqDNA polymerase. 4.4 dNTP. containing dCTP, dGTP, dATP, dTTP each 10mmol/L. 4.5 M-MLV reverse transcriptase. 4.6 RNasin. RNA inhibitors. 4.7 primer. The selected genes ISAV eighth fragment. Primers synthesized DNA enzyme-free, ultra-pure water without RNA enzyme will lead With the probe was formulated as 100μmol/L stock solution, set -20 ℃ or below freezing; take appropriate formulated as 20μmol/L at work use ......

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