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Quarantine protocal for peste des petits ruminants
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Basic data
| Standard ID | SN/T 2733-2010 (SN/T2733-2010) |
| Description (Translated English) | Quarantine protocal for peste des petits ruminants |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Classification of International Standard | 11.220 |
| Word Count Estimation | 18,148 |
| Date of Issue | 2010-11-01 |
| Date of Implementation | 2011-05-01 |
| Quoted Standard | GB/T 18088 |
| Regulation (derived from) | National Quality Inspection (2010) 582 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the peste des petits ruminants Isolation and identification of pathogens, polymerase chain reaction, serum antibody neutralization test, enzyme-linked immunosorbent assay method. This standard applies to peste des petits ruminants in the diagnosis and epidemiological investigation. |
SN/T 2733-2010: Quarantine protocal for peste des petits ruminants
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Quarantine protocal for peste des petits ruminants
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Peste des petits ruminants quarantine Technical Specifications
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Yunnan Exit Inspection and Quarantine, Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine
Bureau, People's Republic of China Tibet Exit Inspection and Quarantine.
The main drafters of this standard. Dong Jun, Yang Jianmin, strict tree hair, Yang Yunqing, Ai Jun, Ye Lingling, Xu Zizhong, flower Qun Yi, Zhou Xiaoli.
Peste des petits ruminants quarantine Technical Specifications
1 Scope
This standard specifies the Isolation and identification of PPR, the polymerase chain reaction, and serum antibody test, ELISA
Test detection method.
This standard applies to PPR diagnosis and epidemiological investigation.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Peste des petits ruminants pestedespetitsruminants; PPR
One kind of acute viral infection caused by peste des petits ruminants caused by a virus, infects small ruminants, fever, stomatitis, diarrhea, pneumonia
Characterized.
4 disease profile
See Appendix A.
5 on-site inspection and quarantine and sampling
5.1 on-site inspection and quarantine. first by clinical examination, make a preliminary diagnosis based on clinical manifestations and specific pathological changes.
5.2 Sampling. According to GB/T 18088 regulations by sampling head, fill in accordance with the provisions "to send samples list" sent to the laboratory.
6 Diagnosis
6.1 Virus isolation and identification (three biosafety laboratory)
6.1.1 Reagents and materials
6.1.1.1 Peste des petits ruminants standard positive and negative serum.
6.1.1.2 Cell. Yang Shen primary cells or African green monkey kidney cells (VERO).
6.1.1.3 broth.199 or MEM medium with 10% calf serum (by 56 ℃ 30 min off energy), containing penicillin
200IU/mL, streptomycin 200IU/mL.
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