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SN/T 2660-2010: Preservation methods for culture collection in foodstuff microbiology laboratory
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SN/T 2660-2010: Preservation methods for culture collection in foodstuff microbiology laboratory

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Preservation methods for culture collection in foodstuff microbiology laboratory People's Republic of China Entry-Exit Inspection and Quarantine Standards Food Microbiology Laboratory Culture Collection Methods Issued on. 2010-11-01 2011-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine. The main drafters. Zhang Haibin, Gao Qi Li, Zhang Xia, Liu Pei, Wangnai Fu, Zhang Haiying Wu winter snows. Food Microbiology Laboratory Culture Collection Methods

1 Scope

This standard specifies the food microbiology laboratory common definition of culture collections, technical principles, technical requirements and technical methods. This standard applies to food microbiology laboratory species preservation.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 19489 General requirements for laboratory biosafety Pathogenic microbiology laboratory bio-safety regulations List of pathogenic organisms transmissible between humans Animal pathogens classified directory

3 Definitions

The following terms and definitions apply to this document. 3.1 Culture Collection culturepreservation The microbial strains with a variety of suitable methods proper preservation, to avoid death, pollution, maintaining its original traits stable. 3.2 Regular transplantation periodicalsubculturing Also known as subculture preservation law, including slant culture, stab culture, liquid culture and so on. The inoculated in a suitable medium, optimum Cultured under conditions, sufficient to be grown after save at 4 ℃ ~ 6 ℃ certain time interval and a transplantation of cultured Culture Collection method. 3.3 Nujol preservationinliquidparaffin Preservation method, also known as mineral oil, regular transplant preservation adjunct law. The inoculated slant on a suitable medium, in optimum conditions After cultured to grow healthy bacteria colonies inject sterile liquid paraffin, it covers the entire slope, and then placed upright in a low temperature (4 ℃ ~ 6 ℃) dry place to save a species preservation methods. 3.4 Porcelain beads preservation method porousbeadspreservation The cultured microbial cells or spores made good suspension, into the porous containing sterile glass beads (or ceramic beads) in a sterile vial to suck Glass beads attached to the surface, removal of one strain preservation method excess suspension, Cryopreservation. 3.5 Freeze drying preservation method of freeze-drying The principle is the microbial cells in the frozen, reduced pressure, the occurrence of "sublimation" dehydration, cell physiological activity tends to stop, long-term maintenance Viable state. ...