SN/T 2653-2010 English PDFUS$239.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 2653-2010: Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in papaya Status: Valid
Basic dataStandard ID: SN/T 2653-2010 (SN/T2653-2010)Description (Translated English): Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in papaya Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: X24 Classification of International Standard: 67.080.01 Word Count Estimation: 9,917 Date of Issue: 2010-11-01 Date of Implementation: 2011-05-01 Quoted Standard: GB/T 6682; SN/T 1193; SN/T 1194 Regulation (derived from): National Quality Inspection (2010) 582 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the GMO papaya qualitative PCR assay. This standard applies to antiviral qualitative detection of genetically modified papaya. SN/T 2653-2010: Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in papaya---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in papaya People's Republic of China Entry-Exit Inspection and Quarantine Standards Qualitative PCR Detection of Genetically Modified Papaya Protocolofthequalitativepolymerasechainreaction (PCR) fordetecting Issued on. 2010-11-01 2011-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, the People's Republic of China And country SZCIQ, People's Republic of China Beijing Exit Inspection and Quarantine. The main drafters of this standard. Chen Yun, Chen Shuangya, Liang Miao, Conlin, Yezheng Qing, Li Ling, water Zhu Fang, Chen Hongjun. Qualitative PCR Detection of Genetically Modified Papaya1 ScopeThis standard specifies the qualitative PCR detection of genetically modified papaya. This standard applies to anti-viral transgenic papaya qualitative detection.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 6682 analytical laboratory use specifications and test methods SN/T 1193 genetic testing laboratory technical requirements SN/T 1194 to detect genetically modified plants and their products sampling and sample preparation methods3 AbbreviationsThe following abbreviations apply to this document. CP (coatproteingene). coat protein gene CaMV35S (35Spromoterfromcauliflowermosaicvirus). from the cauliflower mosaic virus 35S promoter GMYK. PRSVYK lines CP gene mediated antiviral transgenic papaya lines NPTII (neomycin-3'-phosphotransferasegene). 3'-neomycin phosphotransferase gene NOS (terminatorofnopalinesynthasegene). nopaline synthase gene terminator PRSV (Papayaringspotvirus). papaya ringspot virus Papain. Papain gene, papaya endogenous gene Rep (replicasegene). copy gene 55-1/63-1. PRSVHA5-1 lines CP gene mediated antiviral transgenic papaya lines Huanong No. 1. PRSVRep mediated antiviral gene transgenic papaya lines Principle 4 After extraction of the sample DNA, for foreign genes in transgenic plants of the inserted gene sequences of primers were designed by the PCR technique, Specifically amplified DNA fragment of the foreign gene, based on results of PCR amplification, the determination whether the sample contains GMO.5 Reagents and Instruments5.1 Reagents Unless otherwise specified, all reagents were of analytical grade or biochemical reagents. Water as a water GB/T 6682 regulations. 5.1.1 1mol/LTris · HCl buffer (pH8.0). Weigh 121.1gTris, was dissolved in 800mL of water with stirring, concentrated hydrochloric acid 42mL, cooled to room temperature, dilute hydrochloric acid to adjust the pH to 8.0 accurate, then set the volume to 1L, dispensing after autoclaving spare. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 2653-2010_English be delivered?Answer: Upon your order, we will start to translate SN/T 2653-2010_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 2653-2010_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 2653-2010_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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