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SN/T 2135-2008: Detection of genetically modified components in honey. Contentional PCR and real-time fluorescence PCR
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SN/T 2135-2008: Detection of genetically modified components in honey. Contentional PCR and real-time fluorescence PCR

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Detection of genetically modified components in honey.Contentional PCR and real-time fluorescence PCR Exit inspection and quarantine industry standard book People's Republic of China Honey GMO detection methods Ordinary PCR method and real-time PCR method Posted 2008-09-04 2009-03-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

The Standard Appendix A and Appendix B are normative. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Beijing Exit Inspection and Quarantine drafted. The main drafters of this standard. Rao red, Qian Feng, Chan Kwong-chuen, Fu Pu Bo, Zeng Jing, Wang Qi, Zhang Huiyuan. This standard is the first release of the entry-exit inspection and quarantine industry standards. Honey GMO detection methods Ordinary PCR method and real-time PCR method

1 Scope

This standard specifies the qualitative detection of genetically modified honey. This standard is applicable to transgenic cotton, canola and other transgenic plants as nectar collected by honey or contaminated by genetically modified crops Honey GMO detection.

2 standardized reference documents

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) SN/T 1193 genetic analysis and detection laboratory technical requirements SN/T 1194 to detect genetically modified plants and their products sampling and sample preparation methods 3 Terms, definitions and abbreviations 3.1 Terms and Definitions The following terms and definitions apply to this standard. 3.1.1 In vitro enzymatic synthesis of specific DNA fragments method, the gene sequence template first denatured at high temperature into a single strand, DNA polymerase in And under suitable reaction conditions, according to the template sequence designed two primers complementary to sequences corresponding period of the two strands of the template DNA Annealing occurs in conjunction with each other, followed by the role of DNA polymerase in four deoxyribose (dNTP) as substrates, primer can be extended, Then repeated denaturation, annealing and extension of the cycle, so that the amplified gene fragment want to geometrically amplify. 3.1.2 Join fluorophore in the PCR reaction, fluorescence signal accumulated real-time detection of the PCR process, and finally the standard curve Unknown template qualitative or quantitative analysis method. Adding PCR amplification primers were simultaneously added to a specific fluorescence A probe is a oligonucleotide labeled at both ends of a reporter fluorescent group and a quencher group. Probe is intact, the report Fluorescence signal emitted by the group quencher absorption; PCR amplification, T Rao q enzyme 5'-3 'exonuclease activity of the enzyme degradation of the probe, so that packets Advertisement fluorophore and quencher fluorophore separate, thereby fluorescence detection system may receive the fluorescent signal, that is, each amplified a DNA strand, there A fluorescent molecule is formed to achieve the accumulation of PCR product of the fluorescence signal is formed completely synchronized. It is through the PCR amplification reaction Real-time detection of fluorescent signal in each cycle of the product in order to achieve the analysis of quantitative and qualitative initial template. In real-time PCR Reaction, the introduction of a fluorescent chemical reaction proceeds with PCR, PCR reaction products constantly accumulated, the fluorescence signal intensity, etc. Increase the proportion. After each cycle, the intensity of the fluorescence signal is collected once, so you can monitor changes in the product by varying the amount of fluorescence intensity Technology, thereby obtaining a fluorescence amplification plot. 3.1.3 Genetically Modified samples for testing to determine whether the sample is genetically modified. ...