SN/T 2060-2008 English PDF
Basic dataStandard ID: SN/T 2060-2008 (SN/T2060-2008)Description (Translated English): Determination of tylosin residues in royal jelly for import and export. Enzyme-linked immunosorbent assay Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: X04 Word Count Estimation: 15,123 Date of Issue: 2008-04-29 Date of Implementation: 2008-11-01 Quoted Standard: GB/T 6682 Regulation (derived from): Industry standard filing Notice 2008 No. 7 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the royal jelly and royal jelly lyophilized powder tylosin residues enzyme immunoassay method. This standard applies to royal jelly and royal jelly lyophilized powder tylosin residues. SN/T 2060-2008: Determination of tylosin residues in royal jelly for import and export. Enzyme-linked immunosorbent assay---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of tylosin residues in royal jelly for import and export.Enzyme-linked immunosorbent assay Exit inspection and quarantine industry standard book People's Republic of China Importers and tylosin residues in royal jelly Determination ELISA Posted 2008-04-29 2008-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordAppendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine. The main drafters of this standard. Shi Weiliang, Zhang Xiaofeng, Lihao Yan, Zhu Zhenjiang, He Yongqiang. This standard is the first release of the entry-exit inspection and quarantine industry standards. Importers and tylosin residues in royal jelly Determination ELISA1 ScopeThis standard specifies the method of enzyme-linked immunoassay and royal jelly royal jelly lyophilized tylosin residues. This standard applies to the determination of tylosin residue in royal jelly and lyophilized royal jelly.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequently Some amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Study whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)3 Method summaryAnd adjusted to this standard acidic heptane sulfonate buffer to extract tylosin residue in royal jelly, royal jelly protein precipitation pH 7.0. After processing the samples remaining enzyme-labeled tylosin and tylosin jointly compete for binding to the coated sheep on a microplate Anti-rabbit antibody, to remove unbound enzyme-labeled tylosin and tylosin by washing, followed by addition of chromogenic substrate, the absorbance was measured with a microplate reader Degrees, according to the content of the sample absorbance worth tylosin.4 Reagents and materialsUnless otherwise indicated, all reagents were of analytical grade water as a water GB/T 6682 regulations. 4.1 tylosin detection kit (see Appendix A). 4.2 heptane sulfonate buffer solution. Weigh 10.1g heptane sulfonate [CH3 (CH2) 6SO3Na], 11.4g sodium phosphate (Na3PO4 · 12H2O) dissolved in water and made up to 1L, with phosphoric acid to adjust the pH to 2.0. 4.3 phosphate buffer solution. Weigh sodium phosphate (Na2HPO3 · 12H2O) 34g, dissolved in water and made up to 1L, pH was adjusted to 8.0. 4.4 HLB cartridge, Oasis (or equivalent), 3mL (60mg). 4.5 tylosin Standard. 98% or greater purity. 4.6 tylosin preparation of standard solution. methanol as the solvent formulated to 400μg/mL of the stock solution at -20 ℃ conditions save. 5. Apparatus 5.1 microplate reader. 5.2 eight pipettes. 10μL ~ 100μL. 5.3 Single channel pipettes. 10μL ~ 100μL, 100μL ~ 1000μL and 2mL ~ 10mL. 5.4 hybrid oscillator. 5.5 low-speed centrifuge. 6000r/min. 5.6 stoppered test tube. 50mL. 5.7 Electronic Balance. 0.01g ~ 100g. 5.8 pH meter. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 2060-2008_English be delivered?Answer: Upon your order, we will start to translate SN/T 2060-2008_English as soon as possible, and keep you informed of the progress. The lead time is typically in 9 seconds (download/delivered in 9 seconds). The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 2060-2008_English with my colleagues?Answer: Yes. 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