SN/T 2050-2008 English PDF

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SN/T 2050-2008: Determination of 14 beta-lactam antibiotic residues in foodstuffs of animal origin for export and import-LC-MS/MS method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2050-2008	709	Add to Cart	5 days	Determination of 14 beta-lactam antibiotic residues in foodstuffs of animal origin for export and import-LC-MS/MS method	Valid

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Basic data

Standard ID: SN/T 2050-2008 (SN/T2050-2008)
Description (Translated English): Determination of 14 beta-lactam antibiotic residues in foodstuffs of animal origin for export and import-LC-MS/MS method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X04
Word Count Estimation: 27,222
Date of Issue: 2008-04-29
Date of Implementation: 2008-11-01
Quoted Standard: GB/T 6682
Regulation (derived from): Industry standard filing Notice 2008 No. 7
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the food of animal origin amoxycillin, ampicillin, cloxacillin, dicloxacillin, ethoxycarbonyl naphthylamine penicillin, oxacillin, benzylpenicillin, phenoxymethyl penicillin, benzene microphone penicillin, methicillin, phenoxy ethyl penicillin, cephalexin, Pyrazoline, cefazolin of 14 ��- lactam antibiotics by liquid chromatography tandem mass spectrometry. This standard applies to animal muscle, liver, kidney, milk and eggs, 14 kinds of ��- lactam antibiotics residues detection and confirmation.

SN/T 2050-2008: Determination of 14 beta-lactam antibiotic residues in foodstuffs of animal origin for export and import-LC-MS/MS method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of 14 beta-lactam antibiotic residues in foodstuffs of animal origin for export and import-LC-MS/MS method Exit inspection and quarantine industry standard book People's Republic of China Import and export of food of animal origin of 14 β- lactam Amine amount of antibiotic residues detection method Liquid chromatography - mass spectrometry/mass spectrometry Posted 2008-04-29 2008-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

Appendix A of this standard and Appendix B is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard by the People's Republic of China Liaoning Province Exit Inspection and Quarantine is responsible for drafting. The main drafters of this standard. Linwei Xuan, Li Yichen, in spirit, Mike Sui Tian Miao, Yang Chunguang, Xufeng Min, Wang Hongwei. This standard is the first release of the entry-exit inspection and quarantine industry standards. Import and export of food of animal origin of 14 β- lactam Amine amount of antibiotic residues detection method Liquid chromatography - mass spectrometry/mass spectrometry

1 Scope

This standard specifies the foodstuffs of animal origin amoxycillin, ampicillin, cloxacillin, dicloxacillin, ethoxy naphthylamine Penicillium Su, oxacillin, benzylpenicillin, phenoxymethylpenicillin, benzene microphone penicillin, methicillin, phenoxyethyl penicillin, cephalexin, head Spore Pyrazoline, cefazolin total of 14 kinds of β- lactam antibiotics liquid chromatography tandem mass spectrometry. This standard applies to animal muscle, liver, kidney, milk and eggs of 14 β- lactam antibiotic residues detection and confirmation.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)

3 Method summary

Samples β- lactam antibiotics residue with acetonitrile - aqueous extract, the extract was removed by rotary evaporation in acetonitrile was added phosphate buffer Chong added. Extract with HLB solid phase extraction (SPE), after the eluent nitrogen blow by liquid chromatography tandem mass spectrometry, external standard.

4 Reagents and materials

Unless otherwise specified, the reagents were of analytical grade water as a water GB/T 6682 regulations. 4.1 acetonitrile. HPLC fractions. 4.2 Methanol. HPLC fractions. 4.3 formic acid. HPLC fractions. 4.4 sodium chloride. 4.5 sodium hydroxide. 4.6 potassium dihydrogen phosphate. 4.7 dipotassium hydrogen phosphate. 4.8 SPE column. WatersoasisHLB solid phase extraction column, 500mg, 6mL or equivalent person. Use before using 2mL methanol, 1mL water pretreatment. 4.9 0.1mol/L sodium hydroxide. Weigh 4g of sodium hydroxide, and diluted with water to 1000mL. 4.10 acetonitrile - water. (15 + 2, volume ratio). 4.11 acetonitrile - water. (30 + 70, by volume). 4.12 0.05mol/L phosphate buffer solution (pH8.5). Weigh 8.7g of dipotassium hydrogen phosphate, dissolved in deionised water and diluted to 1000mL, PH was adjusted to 8.5 ± 0.1. 4.13 0.025mol/L phosphate buffer solution (pH7.0). Weigh 3.4g potassium dihydrogen phosphate, ultrapure water dissolved and diluted to 1000mL, tone Section pH to 7.0 ± 0.1. 4.14 0.01mol/L ammonium acetate solution (pH = 4.5). Weigh 0.77g of ammonium acetate, dissolved in deionised water and diluted to 1000mL, formic acid ......

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