SN/T 2058-2008 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2058-2008	639	Add to Cart	3 days	Determination of chloramphenicol residues in royal jelly for import and export. Enzyme-linked immunosorbent assay	Obsolete

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Basic data

Standard ID: SN/T 2058-2008 (SN/T2058-2008)
Description (Translated English): Determination of chloramphenicol residues in royal jelly for import and export. Enzyme-linked immunosorbent assay
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X04
Word Count Estimation: 16,197
Date of Issue: 2008-04-29
Date of Implementation: 2008-11-01
Quoted Standard: GB/T 6682
Regulation (derived from): Industry standard filing Notice 2008 No. 7
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the lyophilized royal jelly and chloramphenicol residues in enzyme-linked immunoassay. This standard applies to royal jelly and lyophilized chloramphenicol residues.

SN/T 2058-2008: Determination of chloramphenicol residues in royal jelly for import and export. Enzyme-linked immunosorbent assay

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Determination of chloramphenicol residues in royal jelly for import and export.Enzyme-linked immunosorbent assay Exit inspection and quarantine industry standard book People's Republic of China Importers and royal jelly Determination of chloramphenicol residues ELISA Posted 2008-04-29 2008-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine. The main drafters. Zhang Xiaofeng, Shi Weiliang, Zhu Zhenjiang, Fang Ying, Dong Qiang. This standard is the first release of the entry-exit inspection and quarantine industry standards. Importers and royal jelly Determination of chloramphenicol residues ELISA

1 Scope

This standard specifies the method of enzyme-linked immunoassay and royal jelly freeze-dried powder of chloramphenicol residues. This standard applies to the determination of chloramphenicol residues in royal jelly and lyophilized powder.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)

3 Method summary

This standard TCA to precipitate the protein and royal jelly extract residues of chloramphenicol, and then HLB purification. Microplates Coated with sheep anti-rabbit IgG antibody was added specific antibody (rabbit anti-chloramphenicol antibody), an enzyme-labeled chloramphenicol, chloramphenicol reference sample or standard After taking liquid, with specific antibody coated sheep anti-rabbit IgG antibody, while the free enzyme-labeled chloramphenicol and chloramphenicol competitive and special Specific antibody binding. Remove unbound enzyme-labeled chloramphenicol and chloramphenicol by washing, followed by addition of chromogenic substrate, the absorbance was measured with a microplate reader Degrees, according to the absorbance of the sample is worth chloramphenicol content.

4 Reagents and materials

Unless otherwise indicated, all reagents were of analytical grade water as a water GB/T 6682 regulations. 4.1 chloramphenicol detection kit (see Appendix A). 4.2 methanol. 4.3 5% TCA. 5.0g TCA with dissolved in water and dilute to 100mL. 4.4 30% methanol. 30.0mL of methanol (4.2) with water volume to 100mL. 4.5 isooctane - a mixture of chloroform. iso-octane, chloroform mixed to 2.3 (volume ratio). 4.6 HLB cartridge. Oasis (or equivalent), 3mL (60mg). Chloramphenicol 4.7 standard. Purity ≥98%. Preparation of standard solution of chloramphenicol 4.8. Weigh 0.01g chloramphenicol standard to methanol to 100mL, the preparation of 100μg/mL of The working fluid at 4 ℃ ~ 8 ℃ preservation conditions. 4.9 blank sample. selection is not exactly known royal jelly samples containing chloramphenicol, as a blank sample. With samples for testing spiked prison Control experiments.

5 Instruments

5.1 microplate reader. 5.2 8 pipettes. 10μL ~ 100μL. 5.3 Single channel pipettes. 10μL ~ 100μL, 20μL ~ 200μL, 100μL ~ 1000μL and 2mL ~ 10mL. 5.4 hybrid oscillator. ......