SN/T 2039-2007 English PDFUS$259.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 2039-2007: Identification of Mediterranean fruit fly, ceratitis capitata (Wiedemann). PCR protocol Status: Valid
Basic dataStandard ID: SN/T 2039-2007 (SN/T2039-2007)Description (Translated English): Identification of Mediterranean fruit fly, ceratitis capitata (Wiedemann). PCR protocol Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 07.080 Word Count Estimation: 8,855 Date of Issue: 2007-12-24 Date of Implementation: 2008-07-01 Quoted Standard: GB/T 6682 Regulation (derived from): Industry standard filing Notice 2008 No. 2 (No. 98 overall) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the Mediterranean fruit fly conventional PCR and SYBR Green real-time PCR for identification. This standard applies to the entry of fruit, nightshade vegetables (including tomatoes, eggplant and peppers, etc.) and packaging materials, soil, etc., Inspection and Quarantine of the Mediterranean fruit fly eggs, larvae, pupae and adults of the species identification. SN/T 2039-2007: Identification of Mediterranean fruit fly, ceratitis capitata (Wiedemann). PCR protocol---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Identification of Mediterranean fruit fly, ceratitis capitata (Wiedemann) .PCR protocol Exit inspection and quarantine industry standard book People's Republic of China Mediterranean fruit fly quarantine and identification of PCR Method Posted 2007-12-24 2008-07-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China SZCIQ, People's Republic of China Guangdong Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Yang Weidong, Yudao Jian, Hu learning difficult, Conlin, Chen Chi-nan, Zhong Jianzhong, Zhang Guiming, Xu Lang. This standard is the first release of the entry-exit inspection and quarantine industry standards. Mediterranean fruit fly quarantine and identification of PCR Method1 ScopeThis standard specifies the Mediterranean fruit fly SYBRGreen conventional PCR and real-time PCR identification method. This standard applies to the entry of fruit, Solanaceae vegetables (including tomatoes, eggplants and peppers, etc.) and packaging, such as inspection and quarantine soil ground Sea fruit fly eggs, larvae, pupae and adults of the species identification.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequently Some amendments (excluding corrections) or revisions do not apply to this standard, however, encourage the parties to reach an agreement based on the study criteria Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (neq ISO 3696)3 AbbreviationsThe following abbreviations apply to this standard. 3.1 SYBRGreen real-time fluorescence PCR. 3.2 Deoxynucleoside triphosphates. 3.3 Melting curve analysis. 3.4 T Rao enzyme q Thermus aquaticus DNA polymerase. 3.5 Cytochrome oxidase subunit I. 3.6 Cycle threshold. Principle 4 Fly samples (egg, larva, pupa or adult) after extraction of total genomic DNA, the use of specific primers designed by conventional PCR SYBRGreen and real-time fluorescence PCR (SG-PCR) technique, specific amplification Mediterranean fruit fly mtDNA COI gene fragment (343bp), according to the results of PCR amplification (conventional PCR product by gel electrophoresis; SG-PCR by amplification curve and melting curve), ...... |
