SN/T 2022-2007 English PDFUS$259.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 2022-2007: Protocol of polymerase chain reaction (PCR) for Eperythrozoon wenyoni Status: Valid
Basic dataStandard ID: SN/T 2022-2007 (SN/T2022-2007)Description (Translated English): Protocol of polymerase chain reaction (PCR) for Eperythrozoon wenyoni Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.220 Word Count Estimation: 8,880 Date of Issue: 2007-12-24 Date of Implementation: 2008-07-01 Regulation (derived from): Industry standard filing Notice 2008 No. 2 (No. 98 overall) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the temperature Eperythrozoon polymerase chain reaction method. This standard applies to Winchester Eperythrozoon identification and detection. SN/T 2022-2007: Protocol of polymerase chain reaction (PCR) for Eperythrozoon wenyoni---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Protocol of polymerase chain reaction (PCR) for Eperythrozoon wenyoni Exit inspection and quarantine industry standard book People's Republic of China Niu Wen Eperythrozoon polymerase chain reaction Practice Posted 2007-12-24 2008-07-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordAppendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Huazhong Agricultural University. The main drafters of this standard. Li Shuqing, Hu Yongqiang, Zhao Junlong, Wang Qiao whole, Zhifei Chen, Duque, WangGuiJiang, Huan-Zhang Liu. This standard is the first release of the entry-exit inspection and quarantine industry standards. Niu Wen Eperythrozoon polymerase chain reaction Practice1 ScopeThis standard specifies the Winchester Eperythrozoon polymerase chain reaction method. This standard applies to the identification and detection Eperythrozoon body.2 OverviewmwF/mwR3, can be amplified by PCR fragment of 738bp for identification and detection Eperythrozoon body.3 equipment and materials3.1 Equipment 3.1.1 PCR amplification. 3.1.2 electrophoresis. 3.1.3 gel imaging system. 3.1.4 microwave. 3.1.5 adjustable pipette (2μL, 10μL, 100μL, 200μL, 1000μL). 3.1.6 centrifuge. 3.1.7 scales (0.0001g). 3.1.8 water bath. 3.2 Supplies 3.2.1 PCR reaction tube. 3.2.2 1.5mL tubes. 3.2.3 pipette dropper. 3.3 Reagents 3.3.1 triple-distilled water 0.103MPa sterilization 15min. 3.3.2 agarose. 3.3.3 ethidium bromide. 3.3.4 T Rao q enzyme (5U/μL). 3.3.5 10 × PCR reaction buffer. 3.3.6 magnesium chloride (MgCl2) 25mmol/L. 3.3.7 1mmol/LdNTP. 3.3.8 Phenol - chloroform - isoamyl alcohol (25.24.1). 3.3.9 Molecular Weight indicator. 100bp ~ 2000bpDNAMarker. 3.3.10 50mg/mL lysozyme, 10% SDS, 10mg/mL proteinase K, 5mol/L sodium chloride (NaCl), 5% cetyltrimethylammonium Bromide (CTAB), 2mol/L sodium acetate (NaAc), 70% ethanol, 10mg/mL ethidium bromide (EB), Tris- acetate electrophoresis buffer ...... |
