SN/T 2014-2014 English PDFSN/T 2014: Historical versions
Basic dataStandard ID: SN/T 2014-2014 (SN/T2014-2014)Description (Translated English): Detection and identification of Tomato black ring virus Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 07.100.30 Word Count Estimation: 16,172 Date of Issue: 4/9/2014 Date of Implementation: 11/1/2014 Older Standard (superseded by this standard): SN/T 2014-2007 Quoted Standard: SN/T 1840 Regulation (derived from): State inspection certification (2014) 210 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the tomato black ring virus quarantine and identification methods. This standard applies to quarantine and identification of plant seeds, seedlings, tissue culture, vegetative propagation material Tomato black ring virus. SN/T 2014-2014: Detection and identification of Tomato black ring virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.(Tomato black ring virus quarantine and identification methods) People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 2014-2007 Tomato black ring virus quarantine and identification methods Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. Instead of the standard SN/T 2014-2007 "black ring virus quarantine and identification of tomato." This standard compared with SN/T 2014-2007, the main technical changes are as follows. --- Increased IC-RT-PCR detection method; --- Added Appendix A tomato black ring virus Introduction. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Tianjin People's Republic of China Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau. The main drafters. Zhang Yujun, Guo Jingze, Yang Cuiyun, Wang Jincheng, Liu Peng, Liu Fang, Liuyue Ting, Xiahui Juan, Roca phoenix, to Tsui. This standard replaces the standards previously issued as follows. --- SN/T 2014-2007. Tomato black ring virus quarantine and identification methods1 ScopeThis standard specifies the tomato black ring virus quarantine and identification methods. This standard applies to quarantine and identification of plant seeds, seedlings, tissue culture, vegetative propagation material Tomato black ring virus.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 1840 plant virus immune electron microscopy method3 Tomato black ring virus basic informationName. Tomatoblackringvirus Abbreviation. TBRV Taxonomic position. Associated cowpea virus family (Secoviridae), nematodes pass polyhedron virus genus (Nepovirus). Transmission. The virus by nematodes (Longidorusattenuatus and L.elongatus) transmission, mechanical transmission, seeds (and chickweed Soybeans, etc.) can also be transmitted by vegetative propagation material transport over long distances. For additional information, see Appendix A. Tomato black ring virus Principle 4 Biological Characteristics of Tomato black ring virus, serological properties, molecular biology and mitochondrial morphological characteristics (see Appendix A) is to identify the virus Basis. Using DAS-ELISA (double antibody sandwich enzyme-linked immunosorbent assay), immune electron microscopy, RT-PCR (reverse transcriptase polymerase chain reaction Should), IC-RT-PCR (reverse transcriptase polymerase chain reaction immuno-capture) Tomato black ring virus was detected. Using technology to make detection more As a result, a comprehensive judgment whether the sample with tomato black ring virus. 5 equipment, facilities and appliances 5.1 Equipment Microplate reader, electronic balance (1/10000g), PCR instrument, electrophoresis, gel imaging system, micro juicer, water bath, Autoclave, Water meter, a microwave, a magnetic stirrer, a mortar and pestle, low speed centrifuge, high-speed refrigerated centrifuge, bio-incubator, low temperature refrigerator and -80 ℃ Ultra-low temperature freezer. 5.2 Facilities Quarantine greenhouse (10 ℃ ~ 30 ℃). 5.3 Appliances Adjustable micropipette (2μL, 10μL, 100μL, 200μL, 1000μL, 5000μL) and supporting tip, ELISA plates, tubes, RESEARCH ...... |
