SN/T 2008-2007 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2008-2007	679	Add to Cart	3 days	Determination of patulin in fruit juice for import and export. HPLC method	Obsolete

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Basic data

Standard ID: SN/T 2008-2007 (SN/T2008-2007)
Description (Translated English): Determination of patulin in fruit juice for import and export. HPLC method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X24
Classification of International Standard: 67.080.10
Word Count Estimation: 17,118
Date of Issue: 2007-12-24
Date of Implementation: 2008-07-01
Regulation (derived from): Industry standard filing Notice 2008 No. 2 (No. 98 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the patulin in fruit juices by HPLC method. This standard applies to apple juice and pear juice Determination of patulin.

SN/T 2008-2007: Determination of patulin in fruit juice for import and export. HPLC method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of patulin in fruit juice for import and export.HPLC method Exit inspection and quarantine industry standard book People's Republic of China The patulin detection methods import and export fruit juice High Performance Liquid Chromatography Posted 2007-12-24 2008-07-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

The Standard Appendix A and Appendix B is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shaanxi Exit Inspection and Quarantine. The main drafters of this standard. Kong Xianghong, Li Jianhua, love mountain music, Hexue Wen, Zhang Ying, He Qiang. This standard is the first release of the entry-exit inspection and quarantine industry standards. The patulin detection methods import and export fruit juice High Performance Liquid Chromatography

1 Scope

This standard specifies a high performance liquid chromatographic method patulin in fruit juices tested. This standard applies to apple juice and pear juice Determination of patulin content.

2 Method summary

Juice (juice, use the cloud pectinase pretreatment) of patulin ethyl acetate extract was purified with sodium carbonate solution, the by no Dehydrated over anhydrous sodium sulfate, measured by liquid chromatography with UV or diode array detector, external standard.

3 Reagents and materials

Unless otherwise specified, reagents were analytical grade water for ultra-pure water. 3.1 ethanol. liquid chromatography. 3.2 glacial acetic acid. 3.3 acetonitrile. liquid chromatography. 3.4 ethyl acetate. 3.5 potassium dichromate. 3.6 of concentrated sulfuric acid. 0.009mol/L, 1mL of concentrated sulfuric acid is diluted with water to 2L. 3.7 pectinase solution (endogalacturonase). activity was 1400U/g. Activity Definition. pH3.4 and 25 ℃, 1% pectin Solution in enzyme catalysis, within 5min viscosity is reduced by 20% of the required amount of pectinase. 3.8 anhydrous sodium sulfate. After burning 650 ℃ 4h, placed in the dryer set aside. 3.9 Sodium carbonate solution. 15g/L, 1.5g of anhydrous sodium carbonate in 100mL water. 3.10 pH4.0 Water. redistilled water, adjusted with acetic acid to pH 4.0. 3.11 patulin standard. purity greater than or equal to 99%. 3.12 patulin standard stock solution. with 5mL dissolved in ethyl acetate 5mg patulin shifted into 25mL volumetric flask, acetic acid Ethyl ester to the mark, dubbed the concentration of about 200μg/mL standard stock solution stored frozen at -20 ℃. Patulin standard solution Concentration calibration see Appendix A. 3.13 patulin standard working solution. take 500μL via calibrated patulin standard solution (equivalent to 5μg), purged with nitrogen Dry 5mLpH4.0 dissolved in water, formulated at a concentration of 1μg/mL, and then diluted as needed pH4.0 in water to form a suitable concentration of the standard Quasi working fluid.

4 instruments and equipment

4.1 High performance liquid chromatograph. with UV or diode array detector. 4.2 Centrifuge. 5000r/min. 4.3 centrifuge tube. 50mL, stoppered. 4.4 rotary evaporator. ......

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